SIGMA-ALDRICH Material Safety Data Sheet Date Printed: 08/OCT/2003 Date Updated: 11/JUN/2003 Version 1.1 According to 91/155/EEC 1 - Product and Company Information Product Name 5-FLUORO-2'-DEOXYURIDINE Product Number F0503 Company Sigma-Aldrich Chemie GmbH Riedstrasse 2 89555 Steinheim Germany Technical Phone # 49-89-6513(0)-1444 Fax 49-7329-97-2319 Emergency Phone # 49 7329 97 2323 2 - Composition/Information on Ingredients Product Name CAS # EC no (+)-5-FLUORODEOXYURIDINE 50-91-9 200-072-5 Formula C9H11FN2O5 Molecular Weight 246.19 AMU Synonyms Deoxyfluorouridine,2'-Deoxy-5-fluorouridine, 1-beta-D-2'-Deoxyribofuranosyl-5-flurouracil, Floxuridin,Floxuridine, 5-Fluor-1-(beta-2'-deoxyribofuranosyl)pyrimidin-2 ,4(1H,3H)-dion (Czech),Fluorodeoxyuridine, beta-5-Fluoro-2'-deoxyuridine, 5-Fluorodeoxyuridine,5-Fluoro-2-deoxyuridine, 5-Fluorouracil deoxyriboside, 5-Fluorouracil 2'-deoxyriboside, Fluoruridine deoxyribose,FUDR,NSC-27640,Ro 5-0360 3 - Hazards Identification SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT Harmful if swallowed. 4 - First Aid Measures AFTER INHALATION If inhaled, remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give oxygen. AFTER SKIN CONTACT In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and shoes. Call a physician. AFTER EYE CONTACT In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. AFTER INGESTION If swallowed, wash out mouth with water provided person is conscious. Call a physician. 5 - Fire Fighting Measures EXTINGUISHING MEDIA Suitable: Water spray. Carbon dioxide, dry chemical powder, or appropriate foam. SPECIAL RISKS Specific Hazard(s): Emits toxic fumes under fire conditions. SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes. 6 - Accidental Release Measures PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL Evacuate area. PROCEDURE(S) OF PERSONAL PRECAUTION(S) Wear self-contained breathing apparatus, rubber boots, and heavy rubber gloves. METHODS FOR CLEANING UP Sweep up, place in a bag and hold for waste disposal. Avoid raising dust. Ventilate area and wash spill site after material pickup is complete. 7 - Handling and Storage HANDLING Directions for Safe Handling: Do not breathe dust. Avoid contact with eyes, skin, and clothing. Avoid prolonged or repeated exposure. STORAGE Conditions of Storage: Keep tightly closed. 8 - Exposure Controls / Personal Protection ENGINEERING CONTROLS Mechanical exhaust required. Safety shower and eye bath. GENERAL HYGIENE MEASURES Wash thoroughly after handling. PERSONAL PROTECTIVE EQUIPMENT Respiratory Protection: Government approved respirator. Hand Protection: Compatible chemical-resistant gloves. Eye Protection: Chemical safety goggles. 9 - Physical and Chemical Properties Appearance Physical State: Solid Property Value At Temperature or Pressure pH N/A BP/BP Range N/A SIGMA - F0503 www.sigma-aldrich.com Page 2 MP/MP Range 148 °C Flash Point N/A Flammability N/A Autoignition Temp N/A Oxidizing Properties N/A Explosive Properties N/A Explosion Limits N/A Vapor Pressure N/A Partition Coefficient N/A Viscosity N/A Vapor Density N/A Saturated Vapor Conc. N/A Evaporation Rate N/A Bulk Density N/A Decomposition Temp. N/A Solvent Content N/A Water Content N/A Surface Tension N/A Conductivity N/A Miscellaneous Data N/A Solubility N/A 10 - Stability and Reactivity STABILITY Stable: Stable. Materials to Avoid: Strong oxidizing agents, Strong bases. HAZARDOUS DECOMPOSITION PRODUCTS Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide, Nitrogen oxides, Hydrogen fluoride. HAZARDOUS POLYMERIZATION Hazardous Polymerization: Will not occur 11 - Toxicological Information RTECS NUMBER: YU7525000 ACUTE TOXICITY LD50 Oral Rat 215 mg/kg LD50 Intraperitoneal Rat 1600 MG/KG LD50 Oral Mouse 147 mg/kg LD50 Intraperitoneal Mouse 650 MG/KG Remarks: Gastrointestinal:Hypermotility, diarrhea. Gastrointestinal:Nausea or vomiting. SIGMA - F0503 www.sigma-aldrich.com Page 3 SIGNS AND SYMPTOMS OF EXPOSURE To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated. ROUTE OF EXPOSURE Skin Contact: May cause skin irritation. Skin Absorption: May be harmful if absorbed through the skin. Eye Contact: May cause eye irritation. Inhalation: Material may be irritating to mucous membranes and upper respiratory tract. May be harmful if inhaled. Ingestion: Harmful if swallowed. TARGET ORGAN INFORMATION Bone marrow. G.I. System. Vascular system. Immune system. Heart. CHRONIC EXPOSURE - MUTAGEN Result: Laboratory experiments have shown mutagenic effects. Human 24 MG/L Cell Type: Other cell types DNA damage Human 1 MMOL/L Cell Type: Other cell types DNA damage Human 500 NMOL/L Cell Type: leukocyte DNA Human 100 UMOL/L Cell Type: mammary gland DNA inhibition Human 100 NMOL/L Cell Type: HeLa cell Unscheduled DNA synthesis Human 10 UMOL/L Cell Type: lymphocyte DNA inhibition Human 1 MG/L Cell Type: Other cell types Other mutation test systems Human 500 UG/L Cell Type: Other cell types DNA inhibition Human 10 UMOL/L Cell Type: HeLa cell SIGMA - F0503 www.sigma-aldrich.com Page 4 DNA inhibition Human 25 MG/L Cell Type: lymphocyte DNA inhibition Human 1 UMOL/L Cell Type: Other cell types DNA inhibition Human 1 UMOL/L Cell Type: Other cell types Other mutation test systems Human 10 MG/L Cell Type: Other cell types Other mutation test systems Human 5 MG/L Cell Type: HeLa cell Other mutation test systems Human 100 UMOL/L Cell Type: mammary gland Other mutation test systems Human 200 UMOL/L 24H Cell Type: leukocyte Cytogenetic analysis Human 200 NMOL/L Cell Type: lymphocyte Cytogenetic analysis Human 50 MG/L Cell Type: leukocyte Cytogenetic analysis Rat 5 MG/L Cell Type: Other cell types DNA inhibition Mouse 500 MG/KG Intraperitoneal Micronucleus test Mouse 100 NMOL/L 24H Cell Type: Embryo SIGMA - F0503 www.sigma-aldrich.com Page 5 Morphological transformation. Mouse 500 UMOL/KG Intraperitoneal Other mutation test systems Mouse 100 MG/KG Intraperitoneal DNA inhibition Mouse 2 UMOL/L 23M Cell Type: fibroblast DNA inhibition Mouse 300 UG/L Cell Type: leukocyte DNA inhibition Mouse 45 MG/KG Intraperitoneal Other mutation test systems Mouse 100 NMOL/L Cell Type: Embryo DNA inhibition Mouse 1 UMOL/L Cell Type: Other cell types DNA inhibition Mouse 58 UMOL/L Cell Type: leukocyte Other mutation test systems Mouse 100 MG/KG Intraperitoneal Cytogenetic analysis Mouse 6 NMOL/L Cell Type: Embryo Mutation in mammalian somatic cells. Hamster 100 MG/L Cell Type: ovary DNA inhibition Hamster 10 UMOL/L Cell Type: fibroblast Cytogenetic analysis SIGMA - F0503 www.sigma-aldrich.com Page 6 Hamster 10 UMOL/L Cell Type: fibroblast Sister chromatid exchange Hamster 200 UG/L Cell Type: ovary Mutation in mammalian somatic cells. Hamster 150 NMOL/L Cell Type: lung Mutation in mammalian somatic cells. CHRONIC EXPOSURE - TERATOGEN Species: Rat Dose: 100 MG/KG Route of Application: Intraperitoneal Exposure Time: (12D PREG) Result: Specific Developmental Abnormalities: Craniofacial (including nose and tongue). Specific Developmental Abnormalities: Other developmental abnormalities. Species: Rat Dose: 25 MG/KG Route of Application: Intraperitoneal Exposure Time: (12D PREG) Result: Specific Developmental Abnormalities: Musculoskeletal system. Species: Rat Dose: 25 MG/KG Route of Application: Intraperitoneal Exposure Time: (10D PREG) Result: Specific Developmental Abnormalities: Central nervous system. Specific Developmental Abnormalities: Eye, ear. Specific Developmental Abnormalities: Musculoskeletal system. Species: Rat Dose: 50 MG/KG Route of Application: Intravenous Exposure Time: (11D PREG) Result: Specific Developmental Abnormalities: Musculoskeletal system. Species: Mouse Dose: 60 MG/KG Route of Application: Intraperitoneal Exposure Time: (9D PREG) Result: Specific Developmental Abnormalities: Central nervous system. Specific Developmental Abnormalities: Craniofacial (including nose and tongue). Specific Developmental Abnormalities: Musculoskeletal system. Species: Mouse Dose: 5 MG/KG Route of Application: Intraperitoneal Exposure Time: (12D PREG) Result: Specific Developmental Abnormalities: Musculoskeletal SIGMA - F0503 www.sigma-aldrich.com Page 7 system. Species: Mouse Dose: 25 MG/KG Route of Application: Intraperitoneal Exposure Time: (10D PREG) Result: Effects on Embryo or Fetus: Fetotoxicity (except death, e.g., stunted fetus). Species: Mouse Dose: 45 MG/KG Route of Application: Unreported Exposure Time: (12D PREG) Result: Effects on Embryo or Fetus: Cytological changes (including somatic cell genetic material). Specific Developmental Abnormalities: Central nervous system. CHRONIC EXPOSURE - REPRODUCTIVE HAZARD Species: Rat Dose: 100 MG/KG Route of Application: Intraperitoneal Exposure Time: (12D PREG) Result: Effects on Fertility: Post-implantation mortality (e.g., dead and/or resorbed implants per total number of implants). Specific Developmental Abnormalities: Craniofacial (including nose and tongue). Species: Rat Dose: 25 MG/KG Route of Application: Unreported Exposure Time: (10D PREG) Result: Effects on Fertility: Litter size (e.g.; # fetuses per litter; measured before birth). Species: Mouse Dose: 10 MG/KG Route of Application: Intraperitoneal Exposure Time: (16D PREG) Result: Effects on Newborn: Biochemical and metabolic. Species: Mouse Dose: 10 MG/KG Route of Application: Intraperitoneal Exposure Time: (8D PREG) Result: Effects on Fertility: Post-implantation mortality (e.g., dead and/or resorbed implants per total number of implants). Specific Developmental Abnormalities: Musculoskeletal system. 12 - Ecological Information No data available. 13 - Disposal Considerations SUBSTANCE DISPOSAL Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Observe all federal, state, and local environmental regulations. SIGMA - F0503 www.sigma-aldrich.com Page 8 14 - Transport Information RID/ADR Non-hazardous for road transport. IMDG Non-hazardous for sea transport. IATA Non-hazardous for air transport. 15 - Regulatory Information CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES INDICATION OF DANGER: Xn Harmful. R-PHRASES: 22 Harmful if swallowed. S-PHRASES: 22 36 Do not breathe dust. Wear suitable protective clothing. 16 - Other Information WARRANTY The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Sigma-Aldrich Inc., shall not be held liable for any damage resulting from handling or from contact with the above product. See reverse side of invoice or packing slip for additional terms and conditions of sale. Copyright 2003 Sigma-Aldrich Co. License granted to make unlimited paper copies for internal use only. DISCLAIMER For R&D use only. Not for drug, household or other uses. SIGMA - F0503 www.sigma-aldrich.com Page 9