1
MATERIAL SAFETY DATA SHEET
IDENTIFICATION
Stratagene Cloning Systems
Date of last update: 01/01/02
11011 N. Torrey Pines Rd.
Phone #: 800-424-5444
La Jolla, CA 92037
Fax #: 858-535-0071
Catalog # : 1062-13
Product Name: Acrylease (catalog # 300132)
CAS # : 64-17-5
HAZARDOUS COMPONENTS
COMPONENTS -Chemical Name & Synonyms
% 
OSHA
PEL
Limits
ACGIH
TLV
OTHER LIMITS
RECOMMENDED
Ethyl Alcohol (Ethanol)
91
8H TWA
1000 ppm
(1900
mg/m
3
1000
ppm
N/A
Sulfuric Acid
1
8Hr.
TWA
1 mg/m
3
TWA
1 mg/m
3
STEL
3 mg/m
3
NIOSH
10Hr. TWA
1 mg/m
3
TOXICITY DATA
(Data presented are a compilation of results for Ethanol and Sulfuric Acid. To the best of our knowledge,
no 
independent studies have been conducted for Acrylease).
Irritation Data: 
Skn-Rbt
400 mg; open MLD
UCDS**
7/22/70
Skn-Rbt
20 mg/24 hr. MOD
85JCAE
-,189,86
Eye-Rbt
500 mg; SEV
AJOPAA 29,1363,46
Eye-Rbt
Eye-Rbt
500 mg/24 hr. MLD
100 mg/4S Rinse MOD
85JCAE
FCTOD7
-,189,86
20,573,82
Toxicity Data:
Orl-Chd
LDLo: 2 gm/kg
ATXKA8
17,183,58
Orl-Hmn
LDLo: 1400 mg/kg
NPIRI*
1,44,74
Scu-Inf
LDLo: 19440 mg/kg
AJCPAI
5,466,35
Orl-Rat
LD
50
: 7060 mg/kg
TXAPA9
16,718,70
Ihl-Rat
LC
50
: 20000 ppm/10hr
NPIRI*
1,44,74
Ipr-Rat
LD
50
: 3600 ug/kg
PHMGBN
2,27,69
Ivn-Rat
LD
50
: 1440 mg/kg
TXAPA9
16,718,70
Iat-Rat
LD
50
: 11 mg/kg
TXAPA9
16,718,70
Orl-Mus
LD
50
: 3450 mg/kg
GISAAA 
32(3),31,67
Ihl-Mus
LD
50
: 39 gm/m
3
/4hr.
GTPZAB
26(8),53,82
Ipr-Mus
LD
50
: 933 mg/kg
SCCUR*
0,5,61
Scu-Mus
LD
50
: 8285 mg/kg
FAONAU
48A,99,70
Ivn-Mus
LD
50
: 1973 mg/kg
HBTXAC
1,128,55
Orl-Rbt
LD
50
: 6300 mg/kg
HBTXAC
1,130,55
Ipr-Rbt
LD
50
: 963 mg/kg
EVHPAZ
61,321,85
2
Inv-Rbt
LD
50
: 2374 mg/kg
EVHPAZ
61,321,85
Orl-Gpg LD
50
: 5560 mg/kg
JHITAB 23,259,41
Ipr-Gpg LD
50
: 3414 mg/kg

EVHPAZ 61,321,85 
Ipr-Ham LD
50
: 5068 mg/kg
EVHPAZ 61,321,85
Ipr-Mam LD
50
: 4300 mg/kg
TXAPA9 13,358,68
Reviews, Standards, and Regulations:
IARC Cancer Review: Animal inadequate evidence. IMEMDT 
Target Organ Data:
Behavioral (Sleep)
Behavioral
(Changes in motor activity)
Behavioral
(Ataxia)
Behavioral
(Antipsychotic)
Behavioral
(Headache)
Behavioral
(Change in psychophysiological tests)
Lungs
Thorax or respiration (chronic or pulmonary edema or congestion)
Lungs
Thorax or respiration (Dyspnae)
Lungs
Thorax or respiration (other changes)
Gastrointestinal
(Alteration in gastric secretion)
Gastrointestinal
(Hypermotility, Diarrhea)
Gastrointestinal
(other changes)
Liver
(Fatty liver degeneration)
Liver (Tumors)
Blood
(Lymphoma, including Hodgkins disease)
Blood
(Other chnages)
Paternal effects
(Testes, epididymis, sperm duct)
Effects on Fertility
(Female fertility index)
Effects on Fertility
(Male fertility index)
Effects on Fertility
(Post implantation mortality)
Effects on Fertility
(other measures of fertility)
Effects on Embryo or Fetus
(Extra embryonic structures)
Effects on Embryo or Fetus
(Cytological changes)
Effects on Embryo or Fetus
(Fetotoxicity)
Effects on Embryo or Fetus
(Fetal Death)
Effects on Embryo or Fetus
(Other effects to embryo or fetus)
Specific Developmental Abnormalities
(Eye, ear)
Specific Developmental Abnormalities
(Craniofacial)
Specific Developmental Abnormalities
(Musculoskeletal system)
Specific Developmental Abnormalities
(Respiratory system)
Effects on Newborn
(Growth statistics)
Tumorigenic
(Equivocal tumorigenic agent by RTECS criteria)
Only selected registry of toxic effects of chemical substances (RTECS) data is presented here. See
actual entry in RTECS for complete information.
3
Health Hazard Data
Acute Effects:
Irritating to eyes, respiratory system and skin.
Chronic Effects:
See above.
First Aid:
Eye Contact: Immediately flush with copious amounts of water for at least 15 minutes. Assure
adequate flushing of the eyes by separating the eyelids with fingers.
Skin Contact: Wash with soap and copious amounts of water.
Inhalation: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult,
give oxygen.
Ingestion: Wash out mouth with water (provided person is conscious). Call a physician.
Physical Data
Boiling point:
78
o
C
Flashpoint: 62
o
F
Specific gravity: 0.816 (H
2
O = 1.0)
Appearance and Odor:
Colorless liquid with a sharp sweet odor.
Fire and Explosion Hazard Data
Extinguishing Media:
Carbon dioxide, dry chemical powder or appropriate foam.
Special Firefighting Procedures:
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes
Use water spray to cool fire-exposed containers.
Flammable liquid.
Unusual Fire and Explosion Hazards:
Vapor may travel considerable distances to source of ignition and flash back.
Reactivity Data
Incompatibilities:
Oxidizing agents
Peroxides
Acids
Acid chlorides
Acid anhydrides
Alkali metals
Ammonia
Moisture
Hazardous Combustion or Decomposition Products:
Toxic Fumes of:
Carbon monoxide, carbon dioxide.
Stability:
Stable 
Hazardous polymerization:
4
Will not occur. 
Spill or Leak Procedures
Steps to be taken if Material is Released or Spilled:
Clear area of unnecessary personnel.
Shut off all sources of ignition.
Wear self-contained breathing apparatus, rubber boots and heavy rubber gloves.
Cover spill with dry lime, sand or soda ash. Place in covered container using non-sparking tools and
transport odors. 
Ventilate area and wash spill site after material pickup is complete.
Waste Disposal Method:
Burn in a chemical incinerator equipped with an afterburner and scrubber, but exert extra care in
igniting as this material is highly flammable.
Observe all federal, state and local environmental regulations.
Precautions to be Taken in Handling and Storage
Label Precautionary Statements:
Flammable (USA Definition)
Highly Flammable (European Definition)
Irritant.
Irritating to eyes, respiratory system and skin.
Keep away from sources of ignition. NO smoking!
Wear lab coat or apron, safety glasses or goggles and impermeable rubber gloves.
Eye wash and shower station should be available.
Do not breath vapor.
Avoid prolonged or repeated exposure.
Wash thoroughly after handling.
Keep container tightly closed.
Keep away from heat, sparks and open flame.
Store in a cool dry place.
The above information is believed to be correct but does not purport to be all inclusive and shall be used
only as a guide. Stratagene shall not be held liable for any damage resulting from handling or from contact
with the above product.