SIGMA-ALDRICH Material Safety Data Sheet Date Printed: 08/OCT/2003 Date Updated: 19/FEB/2003 Version 1.1 According to 91/155/EEC 1 - Product and Company Information Product Name (S)-(+)-CAMPTOTHECIN Product Number 365637 Company Sigma-Aldrich Chemie GmbH Riedstrasse 2 89555 Steinheim Germany Technical Phone # 49-89-6513(0)-1444 Fax 49-7329-97-2319 Emergency Phone # 49 7329 97 2323 2 - Composition/Information on Ingredients Product Name CAS # EC no (S)-(+)-CAMPTOTHECIN 7689-03-4 None Formula C20H16N2O4 Molecular Weight 348.36 AMU Synonyms Camptothecin,Camptothecine,20(S)-Camptothecine, NSC 94600,NSC 100880,21, 22-Secocamptothecin-21-oic acid lactone 3 - Hazards Identification SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT Toxic if swallowed. 4 - First Aid Measures AFTER INHALATION If inhaled, remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give oxygen. AFTER INGESTION If swallowed, wash out mouth with water provided person is conscious. Call a physician. 5 - Fire Fighting Measures EXTINGUISHING MEDIA Suitable: Water spray. Carbon dioxide, dry chemical powder, or appropriate foam. SPECIAL RISKS Specific Hazard(s): Emits toxic fumes under fire conditions. SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes. 6 - Accidental Release Measures PERSONAL PRECAUTION PROCEDURES TO BE FOLLOWED IN CASE OF LEAK OR SPILL Evacuate area. PROCEDURE(S) OF PERSONAL PRECAUTION(S) Wear self-contained breathing apparatus, rubber boots, and heavy rubber gloves. Wear disposable coveralls and discard them after use. METHODS FOR CLEANING UP Sweep up, place in a bag and hold for waste disposal. Avoid raising dust. Ventilate area and wash spill site after material pickup is complete. 7 - Handling and Storage HANDLING Directions for Safe Handling: Do not breathe dust. Do not get in eyes, on skin, on clothing. Avoid prolonged or repeated exposure. STORAGE Conditions of Storage: Keep tightly closed. Store in a cool dry place. 8 - Exposure Controls / Personal Protection ENGINEERING CONTROLS Use only in a chemical fume hood. Safety shower and eye bath. GENERAL HYGIENE MEASURES Wash thoroughly after handling. Wash contaminated clothing before reuse. 9 - Physical and Chemical Properties Appearance Color: Light yellow Form: Powder Property Value At Temperature or Pressure pH N/A BP/BP Range N/A MP/MP Range 260 °C Flash Point N/A Flammability N/A Autoignition Temp N/A Oxidizing Properties N/A Explosive Properties N/A Explosion Limits N/A Vapor Pressure N/A Partition Coefficient N/A Viscosity N/A Vapor Density N/A Saturated Vapor Conc. N/A Evaporation Rate N/A Bulk Density N/A Decomposition Temp. N/A Solvent Content N/A Water Content N/A Surface Tension N/A Conductivity N/A ALDRICH - 365637 www.sigma-aldrich.com Page 2 Miscellaneous Data N/A Solubility N/A 10 - Stability and Reactivity STABILITY Materials to Avoid: Strong oxidizing agents. HAZARDOUS DECOMPOSITION PRODUCTS Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide, Nitrogen oxides. 11 - Toxicological Information RTECS NUMBER: UQ0492000 ACUTE TOXICITY LD50 Oral Rat 153 mg/kg LD50 Intravenous Rat 234 MG/KG LD50 Oral Mouse 50.1 mg/kg LD50 Intraperitoneal Mouse 64 MG/KG Remarks: Tumorigenic:Active as anti-cancer agent. LD50 Intravenous Mouse 38 MG/KG SIGNS AND SYMPTOMS OF EXPOSURE To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated. ROUTE OF EXPOSURE Skin Absorption: May be harmful if absorbed through the skin. Inhalation: Material is irritating to mucous membranes and upper respiratory tract. May be harmful if inhaled. Ingestion: Toxic if swallowed. Multiple Routes: Causes eye and skin irritation. CHRONIC EXPOSURE - MUTAGEN Human Human 1 UMOL/L 1 UMOL/L Cell Type: lymphocyte lymphocyte DNA damage DNA damage ALDRICH - 365637 www.sigma-aldrich.com Page 3 Human Human 20 UMOL/L 20 UMOL/L Cell Type: HeLa cell HeLa cell DNA damage DNA damage Human Human 2500 NMOL/L 2500 NMOL/L Cell Type: fibroblast fibroblast DNA damage DNA damage Human Human 2500 NMOL/L 2500 NMOL/L Cell Type: Other cell types Other cell types DNA damage DNA damage Human Human 100 NMOL/L 100 NMOL/L Cell Type: leukocyte leukocyte DNA inhibition DNA inhibition Human Human 5 UMOL/L 5 UMOL/L Cell Type: HeLa cell HeLa cell DNA inhibition DNA inhibition Human Human 5 UMOL/L 5 UMOL/L Cell Type: HeLa cell HeLa cell Other mutation test systems Other mutation test systems Human Human 100 NMOL/L 100 NMOL/L Cell Type: leukocyte leukocyte Other mutation test systems Other mutation test systems Human Human 10 NMOL/L 10 NMOL/L Cell Type: lymphocyte lymphocyte Cytogenetic analysis Cytogenetic analysis Human Human 50 NMOL/L 50 NMOL/L Cell Type: Other cell types Other cell types Cytogenetic analysis Cytogenetic analysis Human Human 1 NMOL/L 1 NMOL/L Cell Type: lymphocyte lymphocyte Sister chromatid exchange Sister chromatid exchange Mouse Mouse 100 UMOL/L 100 UMOL/L Cell Type: leukocyte leukocyte DNA damage DNA damage Mouse Mouse 1 UMOL/L 1 UMOL/L Cell Type: leukocyte leukocyte DNA inhibition DNA inhibition Mouse Mouse 1 MG/L 1 MG/L ALDRICH - 365637 www.sigma-aldrich.com Page 4 Cell Type: lymphocyte lymphocyte DNA inhibition DNA inhibition Mouse Mouse 1 MG/L 1 MG/L Cell Type: lymphocyte lymphocyte Other mutation test systems Other mutation test systems Mouse Mouse 3 MG/KG 3 MG/KG Intraperitoneal Intraperitoneal Cytogenetic analysis Cytogenetic analysis Hamster Hamster 250 NMOL/L 250 NMOL/L Cell Type: lung lung DNA damage DNA damage Hamster Hamster 1 UMOL/L 1 UMOL/L Cell Type: ovary ovary DNA damage DNA damage Hamster Hamster 1 UMOL/L 1 UMOL/L Cell Type: ovary ovary Cytogenetic analysis Cytogenetic analysis Hamster Hamster 100 UG/L 100 UG/L Cell Type: lung lung Cytogenetic analysis Cytogenetic analysis Hamster Hamster 1 UMOL/L 1 UMOL/L Cell Type: lung lung Sister chromatid exchange Sister chromatid exchange Hamster Hamster 5 UMOL/L 5 UMOL/L Cell Type: ovary ovary Sister chromatid exchange Sister chromatid exchange Hamster Hamster 100 NMOL/L 100 NMOL/L Cell Type: lung lung Mutation in mammalian somatic cells. Mutation in mammalian somatic cells. Chicken Chicken 500 UG/L 500 UG/L Cell Type: Embryo Embryo DNA inhibition DNA inhibition Monkey Monkey 25 UMOL/L 25 UMOL/L Cell Type: kidney kidney DNA damage DNA damage 12 - Ecological Information No data available. ALDRICH - 365637 www.sigma-aldrich.com Page 5 13 - Disposal Considerations SUBSTANCE DISPOSAL Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Observe all federal, state, and local environmental regulations. 14 - Transport Information RID/ADR UN#: 1544 Class: 6.1 PG: III Proper Shipping Name: Alkaloids, solid, n.o.s. IMDG UN#: 1544 Class: 6.1 PG: III EmS Number: 6.1-04 MFAG Number: 805 Proper Shipping Name: Alkaloids, solid, n.o.s. Marine Pollutant: No Severe Marine Pollutant: No Technical Name: X IATA UN#: 1544 Class: 6.1 PG: III Proper Shipping Name: Alkaloids, solid, n.o.s. Inhalation Packing Group I: No Technical Name: X 15 - Regulatory Information CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES INDICATION OF DANGER: T Toxic. R-PHRASES: 25 Toxic if swallowed. S-PHRASES: 45 In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). 16 - Other Information WARRANTY The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Sigma-Aldrich Inc., shall not be held liable for any damage resulting from handling or from contact with the above product. See reverse side of invoice or packing slip for additional terms and conditions of sale. Copyright 2003 Sigma-Aldrich Co. License granted to make unlimited paper copies for internal use only. DISCLAIMER For R&D use only. Not for drug, household or other uses. ALDRICH - 365637 www.sigma-aldrich.com Page 6