sn-1,2-Diacylglycerol Kinase, Recombinant, 
Escherichia coli 
Cat. No. 266724 
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Germany 
Tel 0800 6931 000 
E-mail address for technical inquiries: technical@calbiochem.com 
Find our current product data sheets on the web: http://www.calbiochem.com 
Size 
500 ¦Ìg 
1 mg 
DAG Kinase 
Membrane-bound protein that catalyzes the ATP-dependent 
phosphorylation of 1,2-diacyl-sn-glycerol (DAG). The enzyme is specific 
for the sn-1,2 isomer of diacylglycerol. The enzyme will phosphorylate 
ceramide and monoglycerol at a lower rate. Involved in the recycling of 
DAG generated during phospholipid turnover. DAG is a second 
messenger important in the activation of protein kinase C. 
Turbid membrane suspension. Supplied in 10 mM sodium phosphate, 
pH 7.5, 20 mM NaCl, 1 mM DTT, and 20% glycerol. 
Product is overexpressed using recombinant technology in the E. coli 
strain N4830. 
93076-89-2 
2.7.1.107 
13,700 
¡Ý2 units/mg protein 
One unit is defined as the amount of enzyme that will phosphorylate 
1.0 ¦Ìmol of DAG per minute at 25¡ãC, pH 6.6. 
If diluting the enzyme significantly, it may be necessary to add 
0.5-1% n-decyl-¦Â-D-maltopyranoside or another suitable detergent. 
Freezer (-20¡ãC). Avoid freeze/thaw cycles. This product is stable for 2 
years as supplied. As a membrane suspension, the enzyme is stable for 
12 hours at room temperature (+20¡ãC). 
United Kingdom 
Tel 0115 9430 840 
USA & Canada 
Tel (800) 628-8470 
Revised: 29-May-01
CN: 266724 
References: Van Veldhoven, P.P. 1989. Anal. Biochem. 183, 177. 
Preiss, J.E., et al. 1987. Methods Enzymol. 114, 294. 
Bell, R.M. 1986. Cell 45, 631. 
Preiss, J., et al. 1986. J. Biol. Chem. 261, 8597. 
Loomis, C.R., et al. 1985. J. Biol. Chem. 260, 4091. 
FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DRUG USE.