1.
69067 Catalog #
In Case of
Emergency
Enterokinase Cleavage Capture Kit Product name
Product and Company Identification
Material Safety Data Sheet
Call Chemtrec®
(800)424-9300 (within U.S.A.)
(703)527-3887 (outside U.S.A.)
Supplier Manufactured by EMD Biosciences, Inc.
441 Charmany Drive
Madison, WI 53719
(608)238-6110
(800)207-0144
FAX: (608)238-1388
P.O. Box 12087
La Jolla, CA 92039-2087
(858)450-5558
(800)854-3417
FAX: (858)453-3552
Novagen
Order
Number
Customer
Number
Composition and Information on Ingredients
Ethylenediaminetetraacetic Acid 60-00-4 RC096 Xi, N R36, R52/53
Tris Base 77-86-1 RC140 Xi R36/38
Ingredient Name CAS No. R-Phrases
2.
EU Symbol Product No.
Note: See section 8 for occupational exposure limits and section 11 for LC50/LD50 information.
Hazards Identification 3.
Primary Hazards and Critical
Effects
: RC096 WARNING!
HARMFUL IF SWALLOWED.
MAY CAUSE EYE IRRITATION.
HARMFUL TO AQUATIC ORGANISMS.
MAY BE HARMFUL TO ENVIRONMENT IF RELEASED IN LARGE AMOUNTS.
Avoid contact with eyes. Do not ingest. Wash thoroughly after handling. Avoid contact of
spilled material and runoff with soil and surface waterways.
RC140 CAUTION!
MAY CAUSE EYE AND SKIN IRRITATION.
Avoid contact with eyes, skin and clothing. Wash thoroughly after handling.
Environmental Hazards RC096 Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic
environment.
RC096 Irritating to eyes.
RC140 Irritating to eyes and skin.
Human Health Hazards
Physical/Chemical hazards Not applicable :
:
:
RC096 If swallowed, do not induce vomiting unless directed to do so by medical
personnel. Never give anything by mouth to an unconscious person. Get
medical attention immediately.
RC140 Do NOT induce vomiting unless directed to do so by medical personnel.
Never give anything by mouth to an unconscious person. Get medical
attention if symptoms appear.
First Aid Measures 4.
Ingestion
Inhalation RC096, RC140 If inhaled, remove to fresh air. If not breathing, give artificial respiration. If
breathing is difficult, give oxygen. Get medical attention.
:
:
Page: 2/4 Catalog # 69067
Eye Contact
Skin Contact
RC096, RC140 In case of contact, immediately flush eyes with plenty of water for at least 15
minutes. Get medical attention.
RC096, RC140 In case of contact, immediately flush skin with plenty of water. Remove
contaminated clothing and shoes. Wash clothing before reuse. Thoroughly
clean shoes before reuse. Get medical attention.
:
:
Notes to Medical Doctor : Not available.
Fire-Fighting Measures 5.
Fire fighters should wear positive pressure self-contained breathing apparatus (SCBA) and full turnout
gear.
Hazardous Decomposition
Products
Fire/Explosion Hazards
These products are carbon oxides (CO, CO2), nitrogen oxides (NO, NO2...).
Not applicable.
Fire-Fighting Procedures
Use foam or all purpose dry chemicals to extinguish. This material is harmful to aquatic organisms. Fire
water contaminated with this material must be contained and prevented from being discharged to any
waterway, sewer or drain.
:
:
:
: Extinguishing Media
Environmental Precautions
and Clean-up Methods
Personal Precautions
If emergency personnel are unavailable, contain spilled material. For small spills add absorbent (soil may
be used in the absence of other suitable materials) scoop up material and place in a sealed, liquid-proof
container for disposal. For large spills dike spilled material or otherwise contain material to ensure runoff
does not reach a waterway. Place spilled material in an appropriate container for disposal. Minimize
contact of spilled material with soils to prevent runoff to surface waterways.
Immediately contact emergency personnel. Keep unnecessary personnel away. Use suitable protective
equipment (Section 8). Follow all fire fighting procedures (Section 5).
6. Accidental Release Measures
Note: See section 1 for emergency contact information and section 13 for waste disposal.
:
:
Handling
Handling and Storage
Storage
7.
:
RC096 Avoid contact with eyes. Do not ingest. Wash thoroughly after handling. Avoid
contact of spilled material and runoff with soil and surface waterways.
RC140 Avoid contact with eyes, skin and clothing. Wash thoroughly after handling
:
:
Use original container.
Keep container tightly closed. Keep container in a cool, well-ventilated area.
Packaging Materials
Engineering Controls RC096 Open handling must be limited. Use local exhaust ventilation or handle in a ventilated
enclosure.
RC140 No special containment is required. Local exhaust ventilation should be provided.
RC096 Not available.
RC140 Not available.
Ingredient Name Occupational Exposure Limits
Occupational Exposure Limits
8. Exposure Controls and Personal Protection
:
Skin and Body
Personal Protective Equipment
RC096 Use appropriate respiratory protection if there is the potential to exceed the exposure
limit(s).
RC140 Use an approved, properly fitted, HEPA filter cartridge respirator, or a respirator of
greater protection if there is the potential to exceed the exposure limit(s).
:
RC096 Disposable outer garments when there is the potential for contact with the material.
RC140 Work uniform or laboratory coat.
:
: Hands
RC096 Safety glasses. Goggles, face shield, or other full-face protection if potential exists
for direct exposure to aerosols or splashes.
RC140 Safety glasses. Goggles, face shield, or other full-face protection if potential exists
for direct exposure to dust.
: Eyes
Respiratory System
RC096 Use chemical resistant, impervious gloves. Additional body garments
should be used based upon the task being performed (e.g., sleevelets,
apron, gauntlets, disposable suits). Appropriate techniques should be used
to remove potentially contaminated clothing.
RC140 Use latex gloves. Additional body garments should be used based upon the
task being performed (e.g., sleevelets, apron, gauntlets, disposable suits).
Page: 3/4 Catalog # 69067
Physical and Chemical Properties
69066: 1 x 50 U Recombinant Enterokinase
69068: 1 x 1.5 ml EKapture™ Agarose
69069: 1 x 10 ug Cleavage Control Protein, containing Ethylenediaminetetraacetic Acid (RC096)
69070: 1 x 2 ml 1X rEK Dilution/Storage Buffer
69071: 1 x 5 ml 10X rEK Cleavage/Capture Buffer, containing Tris Base (RC140)
69072: 10 each Spin Filters, 2 ml capacity
69074: 2 each Spin Filters, with 5 ml recovery tube
Flash Point
Not available.
9.
Kit Components
Hazardous Decomposition
Products
Conditions and Materials to
Avoid
RC096, RC140 Reactive with oxidizing agents, alkalis.
Stability and Reactivity
Not available.
RC096, RC140 The product is stable. Stability
10.
:
:
:
Toxicological Information 11.
Acute Effects
Eye Contact RC096, RC140 Moderately irritating to the eyes.
RC140 Moderately irritating to the skin. Skin Contact
Not available. Target Organs
Carcinogenic Effects Not available.
Not available.
Developmental and
Teratogenic Effects
Not available.
Mutagenic Effects
Reproductive Effects
Other Information
Not available.
Inhalation
Ingestion
RC096 Repeated exposure to a highly toxic material may produce general deterioration of
health by an accumulation in one or many human organs.
RC140 Repeated or prolonged exposure is not known to aggravate medical condition.
Ingredient Name
RC096 LD50 30 mg/kg Oral Mouse
RC140 LD50
LDLo
5900 mg/kg
1000 mg/kg
Oral
Oral
Rat
Rabbit
RC096 Toxic if swallowed.
RC140 Practically non-toxic if swallowed.
Not available.
Species Route Result Test
Chronic Effects
Toxicity Data
:
:
:
:
:
:
:
:
Adverse Effects : Not available.
Routes of Entry Absorbed through skin. Eye contact. :
:
:
Ecological Information 12.
RC096 Not available. Not available. Not available.
RC140 Not available. Not available. Not available.
Species Period Result
Ecotoxicity Data
Ingredient Name
Page: 4/4 Catalog # 69067
Disposal Consideration 13.
Waste Handling and Disposal Waste must be disposed of in accordance with federal, state and local environmental control regulations. :
Transport Information 14.
Air
IATA-DGR Class Not controlled under IATA. :
Packing Group
Regulatory Information 15.
Safety Phrases S26- In case of contact with eyes, rinse immediately with plenty of water and
seek medical advice.
S61- Avoid release to the environment. Refer to special instructions/Safety
data sheets.
R36/38- Irritating to eyes and skin.
R52/53- Harmful to aquatic organisms, may cause
long-term adverse effects in the aquatic environment.
:
:
:
Hazard Symbol(s)
Risk Phrases
EU Regulations
US Regulations
CLASS: Highly toxic.
State Not available.
TSCA 8(b) inventory: Tris Base
Haz-Com Standard
EPA
:
:
:
Canadian Regulations
WHMIS
Provincial :
:
: CLASS D-1A: Material causing immediate and serious toxic effects (VERY TOXIC).
CEPA DSL: Tris Base
No products were found.
CEPA
Xi, N
Other Information
4/17/2003.
16.
: Date of Printing
1.0 : Version
To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries
assumes any liability whatsoever for the accuracy or completeness of the information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used
with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.
Notice to Reader
Validated by so2 on 4/16/2003.