Conforms to 93/112/EC and ISO 11014-1 Section 1. Chemical Product and Company Identification Product name GelCode Blue Stain Reagent, 30ml Sample Product No. 1856170 Supplier In USA: In Europe: Pierce Perbio Science P.O. Box 117 Industriezone III Rockford, IL 61105 Industrielaan 27 USA 815.968.0747 or 1.800.874.3723 In Case of Emergency CALL CHEMTREC: Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. Section 2. Composition, Information on Ingredients Substance/Preparation Ingredient Name 1) Ingredient 1 2) Ingredient 3 3) Ingredient 4 4) Acid Preparation : CAS No. % 3-5 0.1-1 1-3 7-10 Section 3. Hazards Identification United States Review the most current and approved institutional guideline, protocol, standard operating procedure(s) and MSDS(s) for the proper handling of institutional materials/equipment associated with the use of this product. Emergency Overview DANGER! Routes of Entry Potential Acute Health Effects Carcinogenic Effects Data Medical Conditions Aggravated by Overexposure: Overexposure /Signs/Symptoms Europe CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: LUNGS, GASTROINTESTINAL TRACT, RESPIRATORY TRACT, SKIN, EYES, CENTRAL NERVOUS SYSTEM, EYE, LENS OR CORNEA. MAY BE HARMFUL IF INHALED, ABSORBED THROUGH SKIN OR SWALLOWED. Avoid prolonged contact with eyes, skin, and clothing. Do not ingest. Avoid breathing vapor or mist. Keep container closed. Use only with adequate ventilation. Wash thoroughly after handling. Absorbed through skin. Eye contact. Inhalation. Ingestion. Slightly hazardous in case of skin contact (corrosive, irritant), of eye contact (irritant, corrosive), of ingestion, of inhalation (lung irritant, lung corrosive). Liquid or spray mist may produce tissue damage particularly on mucous membranes of eyes, mouth and respiratory tract. Skin contact may produce burns. Inhalation of the spray mist may produce severe irritation of respiratory tract, characterized by coughing, choking, or shortness of breath. CARCINOGENIC EFFECTS: Classified 4 (No evidence.) by NTP, None. by OSHA, None. by NIOSH [Ingredient 1]. Classified 4 (No evidence.) by NTP, None. by OSHA [Ingredient 3]. Classified None. by NIOSH [Ingredient 4]. MUTAGENIC EFFECTS: Mutagenic for bacteria and/or yeast. [Ingredient 4]. TERATOGENIC EFFECTS: Not available. Repeated or prolonged contact with spray mist may produce chronic eye irritation and severe skin irritation. Repeated or prolonged exposure to spray mist may produce respiratory tract irritation leading to frequent attacks of bronchial infection. Not available. Manufacturer Pierce Biotechnology P.O. Box 117 Rockford, IL 61105 USA 815.968.0747 or 1.800.874.3723 9320 Erembodegem-Aalst Belgium Tel:+32 53 83 44 04 Fax:+32 53 83 76 38 Print Date 11/5/2002 Validation Date 11/4/2002 MSDS# 3811 800.424.9300 OUTSIDE US: 202.483.7616 EC Number Continued on Next Page Eng Material Safety Data Sheet Responsible Name Pierce Administration Symbol R-Phrases F, T -- Xi Xn R11, R23/24/25, R39/23/24/25 Not controlled under dsd (Europe). R36/38 R22, R37/38, R41 Page: 2/6 GelCode Blue Stain Reagent, 30ml Sample Classification R20/21/22, R40/20/21/22 Physical/chemical hazards Human health hazards Not applicable. Harmful by inhalation, in contact with skin and if swallowed. Harmful: possible risk of irreversible effects through inhalation, in contact with skin and if swallowed. Environmental hazards Not applicable. See Toxicological Information (section 11) Section 4. First Aid Measures Notice to Reader Get immediate medical attention. Effects and symptoms Inhalation Slightly hazardous in case of inhalation (lung irritant, lung corrosive). Inhalation of the spray mist may produce severe irritation of respiratory tract, characterized by coughing, choking, or shortness of breath. Over-exposure by inhalation may cause respiratory irritation. Ingestion May be fatal if swallowed. May cause burns to mouth, throat, and stomach. Skin Contact Sensitization of the product: Not available. Slightly hazardous in case of skin contact (corrosive, irritant). Skin contact may produce burns. Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering. Eye Contact Slightly hazardous in case of eye contact (irritant, corrosive). Aggravating conditions Repeated or prolonged contact with spray mist may produce chronic eye irritation and severe skin irritation. Repeated or prolonged exposure to spray mist may produce respiratory tract irritation leading to frequent attacks of bronchial infection. First-Aid Measures Inhalation If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention. Ingestion Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. If large quantities of this material are swallowed, call a physician immediately. Loosen tight clothing such as a collar, tie, belt or waistband. Skin Contact In case of contact, immediately flush skin with plenty of water. Cover the irritated skin with an emollient. Remove contaminated clothing and shoes. Cold water may be used.Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention. Eye Contact Check for and remove any contact lenses. Immediately flush eyes with running water for at least 15 minutes, keeping eyelids open. Cold water may be used. Get medical attention if irritation occurs. Notes to Physician Not available. Protection of first-aiders Not available. Section 5. Fire Fighting Measures Flammability of the Product Non-flammable. Flash Points Not applicable. Fire Hazards in Presence of Various Not applicable. Substances Fire Fighting Media Not applicable. and Instructions Protective Clothing (Fire) Not applicable. Hazardous thermal (de)composition Not applicable. products Section 6. Accidental Release Measures Personal precautions Face shield. Full suit. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Gloves. Boots. Environmental Precautions and Corrosive liquid. Clean-up Methods Stop leak if without risk. Absorb with DRY earth, sand or other non-combustible material. Do not get water inside container. Do not touch spilled material. Use water spray curtain to divert vapor drift. Prevent entry into sewers, basements or confined areas; dike if needed. Call for assistance on disposal. Be careful that the product is not present at a concentration level above TLV. Check TLV on the MSDS and with local authorities. Small Spill and Leak Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container. Continued on Next Page GelCode Blue Stain Reagent, 30ml Sample Section 7. Handling and Storage Handling Avoid prolonged contact with eyes, skin, and clothing. Do not ingest. Avoid breathing vapors or spray mists. Keep container closed. Use only with adequate ventilation. Wash thoroughly after handling. Storage Keep container tightly closed. Keep container in a cool, well-ventilated area. Store between 2 to 8°C (35.6 to 46.4°F). Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. Packaging materials Suitable / Not suitable Use original container. United States 1) Ingredient 1 2) Acid Sweden 1) Ingredient 1 2) Ingredient 3 3) Ingredient 4 4) Acid Denmark 1) Ingredient 1 2) Ingredient 3 3) Ingredient 4 4) Acid Norway 1) Ingredient 1 2) Ingredient 3 3) Ingredient 4 4) Acid France 1) Ingredient 1 2) Ingredient 3 3) Ingredient 4 4) Acid Netherlands 1) Ingredient 1 2) Ingredient 3 3) Ingredient 4 4) Acid Section 8. Exposure Controls/Personal Protection Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective threshold limit value. Ensure that eyewash stations and safety showers are proximal to the work-station Exposure Limit Values Ingredient Name location. Occupational Exposure Limits Not available. TWA: 1 STEL: 3 (mg/m3) from OSHA (PEL) [United States] [1989] Inhalation TWA: 200 STEL: 350 CEIL: 250 (ppm) from AFS [Sweden] [1996] SKIN Not available. TWA: 50 STEL: 500 CEIL: 150 (ppm) from AFS [Sweden] [1996] SKIN Not available. TWA: 200 (ppm) from Arbejdstilsynet [Denmark] [1996] SKIN Not available. TWA: 100 (ppm) from Arbejdstilsynet [Denmark] [1996] Not available. TWA: 100 STEL: 325 (ppm) from Arbeidstilsynet [Norway] [1996] SKIN Not available. Not available. Not available. TWA: 200 STEL: 1300 (ppm) from INRS [France] [1999] Inhalation Not available. Not available. Not available. TWA: 200 (ppm) from Arbeidsinspectie [Netherlands] [2000] Inhalation Not available. TWA: 50 (ppm) from Arbeidsinspectie [Netherlands] [2000] Inhalation Not available. TWA: 200 STEL: 250 CEIL: 800 (ppm) from BAuA [Germany] [1999] SKIN Not available. TWA: 160 (mg/m3) from BAuA [Germany] [1999] SKIN Not available. Germany 1) Ingredient 1 2) Ingredient 3 3) Ingredient 4 4) Acid Personal Protection Eyes Splash goggles. Body Lab coat. Hands Gloves. Respiratory Respirator is not needed under normal and intended conditions of use, if exposures are kept below established limits. Feet Boots. Continued on Next Page Page: 3/6 Page: 4/6 GelCode Blue Stain Reagent, 30ml Sample Protective Clothing (Pictograms) Not available. Odor Taste Not available. Color Clear Bluish-grey Brown. Section 9. Physical and Chemical Properties Liquid. Physical State and Appearance Not applicable. Molecular Weight Neutral. pH (1% Soln/Water) Boiling/Condensation Point The lowest known value is 64.55°C (148.2°F) (Ingredient 1). Weighted average: 104.28°C (219.7°F) Melting/Freezing Point May start to solidify at 42.27°C (108.1°F) based on data for: Acid. Weighted average: -0.18°C (31.7°F) Specific Gravity Weighted average: 1.03 (Water = 1) Vapor Pressure The highest known value is 12.9 kPa (@ 20°C) (Ingredient 1). Weighted average: 2.47 kPa (@ 20°C) Vapor Density The highest known value is 3.4 (Air = 1) (Acid). Weighted average: 0.87 (Air = 1) Odor Threshold The highest known value is 100 ppm (Ingredient 1) Evaporation Rate 0.36 (Water) compared to Butyl acetate. Viscosity Dynamic: The highest known value is 1.1 cP (Ingredient 4) Ionicity (in Water) Non-ionic. Dispersion Properties See solubility in water, diethyl ether, acetone. Solubility Soluble in cold water, hot water. Partially soluble in diethyl ether, acetone. Section 10. Stability and Reactivity Stability and Reactivity The product is stable. Conditions to avoid Not available. Materials to avoid Reactive with organic materials. Slightly reactive to reactive with oxidizing agents, reducing agents, acids. Hazardous Polymerization Will not occur. Hazardous Decomposition Not applicable. Products Section 11. Toxicological Information Toxicity to Animals Acute oral toxicity (LD50): 100 mg/kg [wild bird species]. (Ingredient 4). Acute dermal toxicity (LD50): 2470 mg/kg [Rabbit]. (Acid). Chronic Effects on Humans CARCINOGENIC EFFECTS: Classified 4 (No evidence.) by NTP, None. by OSHA, None. by NIOSH [Ingredient 1]. Classified 4 (No evidence.) by NTP, None. by OSHA [Ingredient 3]. Classified None. by NIOSH [Ingredient 4]. MUTAGENIC EFFECTS: Mutagenic for bacteria and/or yeast. [Ingredient 4]. Other Toxic Effects on Humans Slightly hazardous in case of eye contact (irritant), of ingestion, of inhalation (lung irritant). Special Remarks on Toxicity to Not available. Animals Special Remarks on Chronic May be Harmful by inhalation, ingestion or skin absorption. May cause irritation. To the best of our knowledge, the Effects on Humans chemical, physical and toxicological properties have not been thoroughly investigated. (Ingredient 2) Special Remarks on Other Toxic To the best of our knowledge, the toxicological properties of this product have not been thoroughly investigated. Effects on Humans Continued on Next Page GelCode Blue Stain Reagent, 30ml Sample Section 12. Ecological Information Mobility Persistence/degradability Bioaccumulative potential Not available. Not available. Not available. Ecotoxicity Not available. Germany water class VCI WGK: No products were found. Section 13. Disposal Considerations Waste Information Waste must be disposed of in accordance with federal, state and local environmental control regulations. Waste Stream Not available. Consult your local or regional authorities. Section 14. Transport Information Contact the supplier for all information regarding the proper transportation method for this material. 1 Section 15. Regulatory Information Label Requirements (Europe) R20/21/22- Harmful by inhalation, in contact with skin and if swallowed. R40/20/21/22- Harmful: possible risk of irreversible effects through inhalation, in contact with skin and if swallowed. S36/37- Wear suitable protective clothing and gloves. Harmful HCS Classification Toxic. Target organ effects. Corrosive material U.S. Federal Regulations TSCA 8(b) inventory: Ingredient 1; Ingredient 2; Ingredient 3; Ingredient 4; Milli-Q Water; Water; Acid TSCA 8(d) H and S data reporting: Ingredient 4; Acid SARA 302/304/311/312 extremely hazardous substances: No products were found. SARA 302/304 emergency planning and notification: No products were found. SARA 302/304/311/312 hazardous chemicals: Ingredient 1; Ingredient 4; Acid SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Ingredient 1: fire, immediate health hazard, delayed health hazard; Ingredient 4: immediate health hazard, delayed health hazard; Acid: immediate health hazard SARA 313 toxic chemical notification and release reporting: Ingredient 1 3.5%; Acid 7.225% Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: Acid Clean air act (CAA) 112 accidental release prevention: Ingredient 1 Clean air act (CAA) 112 regulated flammable substances: Ingredient 1 Clean air act (CAA) 112 regulated toxic substances: No products were found. Continued on Next Page Page: 5/6 Page: 6/6 Fire Hazard National Fire Protection Association (U.S.A.) 0 Health Reactivity 0 2 Section 16. Other Information Health Hazardous Material Information System Fire Hazard Reactivity Personal Protection (U.S.A.) References Not available. Full text of R-Phrases referenced under headings 2 and 3: GelCode Blue Stain Reagent, 30ml Sample WHMIS (Canada) CLASS D-1B: Material causing immediate and serious toxic effects (TOXIC). CLASS D-2A: Material causing other toxic effects (VERY TOXIC). CLASS E: Corrosive liquid. CEPA DSL: Ingredient 1; Ingredient 2; Ingredient 3; Ingredient 4; Milli-Q Water; Water; Acid International Regulations Not available. EINECS DSCL (EEC) R20/21/22- Harmful by inhalation, in contact with skin and if swallowed. R40/20/21/22- Harmful: possible risk of irreversible effects through inhalation, in contact with skin and if swallowed. International Lists Australia (NICNAS): Ingredient 1; Ingredient 2; Ingredient 3; Ingredient 4; Milli-Q Water; Water; Acid Germany water class: Ingredient 1; Ingredient 3; Ingredient 4 VCI WGK: No products were found. Korea (TCCL): Ingredient 1; Ingredient 2; Ingredient 3; Ingredient 4; Milli-Q Water; Water; Acid Philippines (RA6969): Ingredient 1; Ingredient 2; Ingredient 3; Ingredient 4; Milli-Q Water; Water; Acid State Regulations Pennsylvania RTK: Ingredient 1: (environmental hazard, generic environmental hazard); Acid: (environmental hazard) Florida: Ingredient 1 Massachusetts RTK: Ingredient 1; Acid New Jersey: Ingredient 1; Acid California prop. 65: No products were found. * 2 0 0 Toxic by inhalation, in contact with skin and if swallowed. Toxic: danger of very serious irreversible effects through inhalation, in contact with skin and if swallowed. Not applicable. Irritating to eyes and skin. Harmful if ingested. Irritating to respiratory system and skin. Risk of serious damage to eyes. Specific Hazard History of Document Changes Any information changes since last document version are marked with a triangle symbol. Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. Verified by Pierce Administration. Validated by Pierce Administration on 11/4/2002. Date of Previous Issue No Previous Validation Version 0.01 Printed 11/5/2002. Notice to Reader To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.