MDE(tm) HETERODUPLEX CONTROL DNA MDE(tm) HETERODUPLEX CONTROL DNA MSDS Number: H4518 --- Effective Date: 03/27/98 1. Product Identification Synonyms: None CAS No.: Not applicable to mixtures. Molecular Weight: Not applicable to mixtures. Chemical Formula: Not applicable to mixtures. 2. Composition/Information on Ingredients Ingredient CAS No Percent Hazardous --------------------------------------- ------------ ------- --------- Sucrose 57-50-1 1 - 10% Yes C.I. Acid Orange 10 1936-15-8 < 1% No Bromophenol Blue 115-39-9 < 1% No Xylene Cyanole 2650-17-1 < 1% No Water 7732-18-5 70 - 90% No This product contains minute quantities of fragmented DNA. MDE(tm) is a Trademark of AT Biochem, Inc. 3. Hazards Identification Emergency Overview -------------------------- CAUTION! MAY CAUSE IRRITATION TO SKIN AND EYES. Potential Health Effects ---------------------------------- Inhalation: No adverse health effects expected from inhalation. May cause mild irritation to the respiratory tract. Ingestion: Extremely large oral dosages may produce gastrointestinal disturbances. Skin Contact: May cause mild irritation. Prolonged contact may cause skin discoloration. Eye Contact: May cause mild irritation, possible reddening. Chronic Exposure: No information found. Aggravation of Pre-existing Conditions: No information found. 4. First Aid Measures Inhalation: Not expected to require first aid measures. Remove to fresh air. Get medical attention for any breathing difficulty. Ingestion: If large amounts were swallowed, give water to drink and get medical advice. Skin Contact: Immediately flush skin with plenty of water for at least 15 minutes. Remove contaminated clothing and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention if irritation develops. Eye Contact: Immediately flush eyes with plenty of water for at least 15 minutes, lifting upper and lower eyelids occasionally. Get medical attention if irritation persists. 5. Fire Fighting Measures Fire: Not considered to be a fire hazard. Explosion: Not considered to be an explosion hazard. Fire Extinguishing Media: Use any means suitable for extinguishing surrounding fire. Special Information: In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode. 6. Accidental Release Measures Ventilate area of leak or spill. Wear appropriate personal protective equipment as specified in Section 8. Contain and recover liquid when possible. Collect liquid in an appropriate container or absorb with an inert material (e. g., vermiculite, dry sand, earth), and place in a chemical waste container. Do not use combustible materials, such as saw dust. Do not flush to sewer! 7. Handling and Storage Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect against physical damage. Containers of this material may be hazardous when empty since they retain product residues (vapors, liquid); observe all warnings and precautions listed for the product. 8. Exposure Controls/Personal Protection Airborne Exposure Limits: Sucrose: - OSHA Permissible Exposure Limit (PEL) - Respirable fraction: 5 mg/m3 (TWA) Total dust: 15 mg/m3 (TWA) - ACGIH Threshold Limit Value (TLV) - 10 mg/m3 total dust containing no asbestos and <1% crylstalline silica. Ventilation System: Not expected to require any special ventilation. Personal Respirators (NIOSH Approved): If the exposure limit is exceeded, a half-face dust/mist respirator may be worn for up to ten times the exposure limit or the maximum use concentration specified by the appropriate regulatory agency or respirator supplier, whichever is lowest. A full-face piece dust/mist respirator may be worn up to 50 times the exposure limit, or the maximum use concentration specified by the appropriate regulatory agency, or respirator supplier, whichever is lowest. For emergencies or instances where the exposure levels are not known, use a full-facepiece positive-pressure, air-supplied respirator. WARNING: Air-purifying respirators do not protect workers in oxygen-deficient atmospheres. Skin Protection: Wear protective gloves and clean body-covering clothing. Eye Protection: Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and quick-drench facilities in work area. 9. Physical and Chemical Properties Appearance: Dark blue to black liquid. Odor: Odorless. Solubility: Complete (100%) Specific Gravity: No information found. pH: No information found. % Volatiles by volume @ 21C (70F): 0 Boiling Point: No information found. Melting Point: No information found. Vapor Density (Air=1): Not applicable. Vapor Pressure (mm Hg): Not applicable. Evaporation Rate (BuAc=1): No information found. 10. Stability and Reactivity Stability: Stable under ordinary conditions of use and storage. Hazardous Decomposition Products: May form toxic fumes of bromides and oxides of carbon, sulfur, and nitrogen when heated to decomposition. Hazardous Polymerization: Will not occur. Incompatibilities: Oxidizers, sulfuric acid and nitric acid. Conditions to Avoid: Heat, flames, ignition sources and incompatibles. 11. Toxicological Information Sucrose: Oral rat LD50: 29700 mg/kg; investigated as a mutagen, reproductive effector. --------\Cancer Lists\------------------------------------------------------ ---NTP Carcinogen--- Ingredient Known Anticipated IARC Category ------------------------------------ ----- ----------- ------------- Sucrose (57-50-1) No No None C.I. Acid Orange 10 (1936-15-8) No No 3 Bromophenol Blue (115-39-9) No No None Xylene Cyanole (2650-17-1) No No None Water (7732-18-5) No No None 12. Ecological Information Environmental Fate: No information found. Environmental Toxicity: No information found. 13. Disposal Considerations Whatever cannot be saved for recovery or recycling should be managed in an appropriate and approved waste disposal facility. Processing, use or contamination of this product may change the waste management options. State and local disposal regulations may differ from federal disposal regulations. Dispose of container and unused contents in accordance with federal, state and local requirements. 14. Transport Information Not regulated. 15. Regulatory Information --------\Chemical Inventory Status - Part 1\--------------------------------- Ingredient TSCA EC Japan Australia ----------------------------------------------- ---- --- ----- --------- Sucrose (57-50-1) Yes Yes No Yes C.I. Acid Orange 10 (1936-15-8) Yes Yes Yes Yes Bromophenol Blue (115-39-9) Yes Yes Yes Yes Xylene Cyanole (2650-17-1) Yes Yes No Yes Water (7732-18-5) Yes Yes Yes Yes --------\Chemical Inventory Status - Part 2\--------------------------------- --Canada-- Ingredient Korea DSL NDSL Phil. ----------------------------------------------- ----- --- ---- ----- Sucrose (57-50-1) Yes Yes No Yes C.I. Acid Orange 10 (1936-15-8) Yes Yes No No Bromophenol Blue (115-39-9) Yes Yes No Yes Xylene Cyanole (2650-17-1) Yes Yes No No Water (7732-18-5) Yes Yes No Yes --------\Federal, State & International Regulations - Part 1\---------------- -SARA 302- ------SARA 313------ Ingredient RQ TPQ List Chemical Catg. ----------------------------------------- --- ----- ---- -------------- Sucrose (57-50-1) No No No No C.I. Acid Orange 10 (1936-15-8) No No No No Bromophenol Blue (115-39-9) No No No No Xylene Cyanole (2650-17-1) No No No No Water (7732-18-5) No No No No --------\Federal, State & International Regulations - Part 2\---------------- -RCRA- -TSCA- Ingredient CERCLA 261.33 8(d) ----------------------------------------- ------ ------ ------ Sucrose (57-50-1) No No No C.I. Acid Orange 10 (1936-15-8) No No No Bromophenol Blue (115-39-9) No No No Xylene Cyanole (2650-17-1) No No No Water (7732-18-5) No No No Chemical Weapons Convention: No TSCA 12(b): No CDTA: No SARA 311/312: Acute: No Chronic: No Fire: No Pressure: No Reactivity: No (Mixture / Liquid) Australian Hazchem Code: No information found. Poison Schedule: No information found. WHMIS: This MSDS has been prepared according to the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all of the information required by the CPR. 16. Other Information NFPA Ratings: Health: 0 Flammability: 1 Reactivity: 0 Label Hazard Warning: CAUTION! MAY CAUSE IRRITATION TO SKIN AND EYES. Label Precautions: Avoid contact with eyes, skin and clothing. Wash thoroughly after handling. Avoid breathing mist. Keep container closed. Use with adequate ventilation. INTENDED FOR R & D USE ONLY. NOT ON THE TSCA INVENTORY. Label First Aid: In case of contact, immediately flush eyes or skin with plenty of water for at least 15 minutes. Get medical attention if irritation develops or persists. Product Use: Electrophoresis. Note: Chemical substances that are manufactured or imported in small quantities solely for the use in Research & Development are not subject to the notification requirements of the Toxic Substances Control Act (TSCA), 15 USC 2604 (h) et seq. Reference - 40 CFR 720.36 These products are not on the TSCA Inventory. They are used only in Research and Development. Revision Information: MSDS Section(s) changed since last revision of document include: 3, 4, 5, 6, 7, 8, 9, 10, 11, 16. Disclaimer: ************************************************************************************************ Mallinckrodt Baker, Inc. provides the information contained herein in good faith but makes no representation as to its comprehensiveness or accuracy. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this product. Individuals receiving the information must exercise their independent judgment in determining its appropriateness for a particular purpose. MALLINCKRODT BAKER, INC. MAKES NO REPRESENTATIONS OR WARRANTIES, EITHER EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION ANY WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE WITH RESPECT TO THE INFORMATION SET FORTH HEREIN OR THE PRODUCT TO WHICH THE INFORMATION REFERS. ACCORDINGLY, MALLINCKRODT BAKER, INC. WILL NOT BE RESPONSIBLE FOR DAMAGES RESULTING FROM USE OF OR RELIANCE UPON THIS INFORMATION. ************************************************************************************************ Prepared by: Strategic Services Division Phone Number: (314) 539-1600 (U.S.A.)