1.
QIA21 Catalog #
In Case of
Emergency
Klenow FragEL™ DNA Fragmentation Detection Kit Product name
Product and Company Identification
Material Safety Data Sheet
Call Chemtrec®
(800)424-9300 (within U.S.A.)
(703)527-3887 (outside U.S.A.)
Supplier EMD Biosciences, Inc.
10394 Pacific Center Court
San Diego, CA 92121
PHONE: (858)450-5558/(800)854-3417
FAX: (858)453-3552
Order Number Customer Number
Composition and Information on Ingredients
Ethylenediaminetetraacetic Acid 60-00-4 RC0960 Xi R36, R52/53
3,3'-Diaminobenzidine, 4HCl 7411-49-6 RC1180 - -
Tris Base 77-86-1 RC1400 Xi R36/38
Penicillin-Streptomycin RC1890 - -
Phosphate Buffered Saline N/A RC1920 Xi R36/37/38
Proteinase K 39450-01-6 RC6630 Xn R36/37/38, R42
Urea-Hydrogen Peroxide 124-43-6 RC6640 O, C R8, R34
Ingredient Name CAS No. R-Phrases
2.
EU Symbol Product No.
Note: See section 8 for occupational exposure limits and section 11 for LC50/LD50 information.
Hazards Identification 3.
Primary Hazards and Critical
Effects
: RC0960 WARNING!
HARMFUL IF SWALLOWED.
MAY CAUSE EYE IRRITATION.
HARMFUL TO AQUATIC ORGANISMS.
MAY BE HARMFUL TO ENVIRONMENT IF RELEASED IN LARGE AMOUNTS.
Avoid contact with eyes. Do not ingest. Wash thoroughly after handling. Avoid contact of spilled
material and runoff with soil and surface waterways.
RC1180 No specific hazard.
RC1400 CAUTION! MAY
CAUSE EYE AND SKIN IRRITATION.
Avoid contact with eyes, skin and clothing. Wash thoroughly after handling.
RC1890 No specific hazard.
BIRTH DEFECT HAZARD
CONTAINS MATERIAL WHICH CAN CAUSE BIRTH DEFECT.
Avoid exposure during pregnancy.
RC1920 CAUTION! MAY
CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION.
Avoid contact with eyes, skin and clothing. Avoid breathing dust. Keep container closed. Use with
adequate ventilation. Wash thoroughly after handling.
RC6630 WARNING!
MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION.
MAY CAUSE ALLERGIC RESPIRATORY REACTION.
Avoid contact with eyes, skin and clothing. Do not breathe dust. Keep container closed. Use only
with adequate ventilation. Wash thoroughly after handling.
RC6640 DANGER!
CAUSES SEVERE RESPIRATORY TRACT, EYE AND SKIN BURNS.
OXIDIZER.
CONTACT WITH OTHER MATERIAL MAY CAUSE FIRE.
MAY CAUSE DAMAGE TO THE FOLLOWING ORGANS: LUNGS, GASTROINTESTINAL
TRACT, RESPIRATORY TRACT, HEAD.
Store in tightly closed container. Do not get in eyes, on skin or on clothing. Do not breathe dust.
Avoid contact with combustible materials. Keep container closed. Use only with adequate
ventilation. Wash thoroughly after handling.
RC0960 Irritating to eyes.
RC1400 Irritating to eyes and skin.
RC1920 Irritating to eyes, respiratory system and skin.
RC6630 Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation.
RC6640 Causes burns.
Human Health Hazards
Physical/Chemical hazards RC6640 Contact with combustible material may cause fire. :
:
Page: 2/5 Catalog # QIA21
Environmental Hazards RC0960 Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment. :
RC0960 If swallowed, do not induce vomiting unless directed to do so by medical personnel.
Never give anything by mouth to an unconscious person. Get medical attention
immediately.
RC1180, RC1890,
RC1920, RC6630,
RC6640
Do NOT induce vomiting unless directed to do so by medical personnel. Never give
anything by mouth to an unconscious person. If large quantities of this material are
swallowed, call a physician immediately.
RC1400 Do NOT induce vomiting unless directed to do so by medical personnel. Never give
anything by mouth to an unconscious person. Get medical attention if symptoms
appear.
Eye Contact
Skin Contact
RC0960, RC1180,
RC1400, RC1890,
RC1920, RC6630
In case of contact, immediately flush eyes with plenty of water for at least 15
minutes. Get medical attention.
RC6640 In case of contact, immediately flush eyes with plenty of water for at least 15
minutes. Get medical attention immediately.
RC0960, RC1180,
RC1400, RC1890,
RC1920, RC6630
In case of contact, immediately flush skin with plenty of water. Remove
contaminated clothing and shoes. Wash clothing before reuse. Thoroughly clean
shoes before reuse. Get medical attention.
RC6640 In case of contact, immediately flush skin with plenty of water for at least 15 minutes
while removing contaminated clothing and shoes. Wash clothing before reuse.
Thoroughly clean shoes before reuse. Get medical attention immediately.
First Aid Measures 4.
Ingestion
Inhalation RC0960, RC1180,
RC1400, RC1890,
RC1920, RC6630
If inhaled, remove to fresh air. If not breathing, give artificial respiration. If
breathing is difficult, give oxygen. Get medical attention.
RC6640 If inhaled, remove to fresh air. If not breathing, give artificial respiration. If
breathing is difficult, give oxygen. Get medical attention immediately.
:
:
:
:
Notes to Medical Doctor : Not available.
Fire-Fighting Measures 5.
Fire fighters should wear positive pressure self-contained breathing apparatus (SCBA) and full turnout gear.
Hazardous Decomposition
Products
Fire/Explosion Hazards
These products are carbon oxides (CO, CO2), nitrogen oxides (NO, NO2...).
Not applicable.
Fire-Fighting Procedures
Use foam or all purpose dry chemicals to extinguish.
:
:
:
: Extinguishing Media
Environmental Precautions
and Clean-up Methods
Personal Precautions
If emergency personnel are unavailable, contain spilled material. For small spills add absorbent (soil may be
used in the absence of other suitable materials) scoop up material and place in a sealed, liquid-proof container
for disposal. For large spills dike spilled material or otherwise contain material to ensure runoff does not reach a
waterway. Place spilled material in an appropriate container for disposal. Minimize contact of spilled material
with soils to prevent runoff to surface waterways.
Immediately contact emergency personnel. Keep unnecessary personnel away. Use suitable protective
equipment (Section 8). Follow all fire fighting procedures (Section 5).
6. Accidental Release Measures
Note: See section 1 for emergency contact information and section 13 for waste disposal.
:
:
Handling
Handling and Storage
Storage
7.
:
RC0960 Avoid contact with eyes. Do not ingest. Wash thoroughly after handling. Avoid contact of
spilled material and runoff with soil and surface waterways.
RC1180 Wash thoroughly after handling.
RC1400 Avoid contact with eyes, skin and clothing. Wash thoroughly after handling.
RC1890 Avoid exposure during pregnancy.
RC1920 Avoid contact with eyes, skin and clothing. Avoid breathing dust. Keep container closed.
Use with adequate ventilation. Wash thoroughly after handling.
RC6630 Avoid contact with eyes, skin and clothing. Do not breathe dust. Keep container closed.
Use only with adequate ventilation. Wash thoroughly after handling.
RC6640 Store in tightly closed container. Do not get in eyes, on skin or on clothing. Do not breathe
dust. Avoid contact with combustible materials. Keep container closed. Use only with
adequate ventilation. Wash thoroughly after handling.
:
:
Use original container.
Keep container tightly closed. Keep container in a cool, well-ventilated area.
Packaging Materials
Page: 3/5 Catalog # QIA21
Engineering Controls RC0960 Open handling must be limited. Use local exhaust ventilation or handle in a ventilated
enclosure.
RC1180, RC1400,
RC1920, RC6630
No special containment is required. Local exhaust ventilation should be provided.
RC1890, RC6640 Open handling is not permitted. All handling must be performed in a glove box or other
totally enclosed system.
RC0960 Not available.
RC1180 Not available.
RC1400 Not available.
RC1890 Not available.
RC1920 Not available.
RC6630 Not available.
RC6640 Not available.
Ingredient Name Occupational Exposure Limits
Occupational Exposure Limits
8. Exposure Controls and Personal Protection
:
Skin and Body
Personal Protective Equipment
RC0960, RC1180,
RC1890
Use appropriate respiratory protection if there is the potential to exceed the exposure
limit(s).
RC1400, RC1920 Use an approved, properly fitted, HEPA filter cartridge respirator, or a respirator of greater
protection if there is the potential to exceed the exposure limit(s).
RC6630, RC6640 Use an approved, properly fitted, powered-air purifying respirator or a respirator of
equivalent or greater protection.
:
RC0960 Disposable outer garments when there is the potential for contact with the material.
RC1180, RC1400,
RC1920, RC6630
Work uniform or laboratory coat.
RC1890, RC6640 Disposable outer garments, or impervious garments of equal or greater protection.
:
: Hands
RC0960, RC1180,
RC1890
Safety glasses. Goggles, face shield, or other full-face protection if potential exists for
direct exposure to aerosols or splashes.
RC1400, RC1920,
RC6630, RC6640
Safety glasses. Goggles, face shield, or other full-face protection if potential exists for
direct exposure to dust.
: Eyes
Respiratory System
RC0960, RC1180,
RC1890
Use chemical resistant, impervious gloves. Additional body garments should be
used based upon the task being performed (e.g., sleevelets, apron, gauntlets,
disposable suits). Appropriate techniques should be used to remove potentially
contaminated clothing.
RC1400, RC1920,
RC6630
Use latex gloves. Additional body garments should be used based upon the task
being performed (e.g., sleevelets, apron, gauntlets, disposable suits).
RC6640 Use chemical resistant, impervious gloves. Additional body garments should be
used based upon the task being performed (e.g., sleevelets, apron, gauntlets,
disposable suits) to avoid exposed skin surfaces. Appropriate techniques should
be used to remove potentially contaminated clothing.
Physical and Chemical Properties
JA1477: 1 x 0.24 ml Proteinase K (RC6630)
JA1478: 1 x 1 ml 10X Klenow Equilibration Buffer, containing Tris Base (RC1400)
JA1479: 1 x 6 ml Stop Solution, containing Ethylenediaminetetraacetic Acid (RC0960)
JA1480: 1 x 12 ml Blocking Buffer, containing Penicillin-Streptomycin (RC1890) and Phosphate Buffered Saline (RC1920)
JA1481: 1 x 0.125 ml 50X Conjugate
JA1482: 1 x 6 ml Methyl Green Counterstain
JA1483: 3 x 1 ml Klenow Reaction Mix
JA1485: 6 each DAB Tablets, containing 3,3'-Diaminobenzidine, 4HCl (RC1180)
JA1486: 6 each H2O2/Urea Tablets (RC6640)
JA1487: 1 x 0.08 ml Klenow Enzyme
JA1609: 2 each Control Slides
Flash Point
Not available.
9.
Kit Components
Page: 4/5 Catalog # QIA21
Hazardous Decomposition
Products
Conditions and Materials to
Avoid
RC0960, RC1400 Reactive with oxidizing agents, alkalis.
RC1180 Reactive with oxidizing agents.
RC6640 Reactive with reducing agents, metals.
Stability and Reactivity
Not available.
RC0960, RC1180, RC1400, RC1890,
RC1920, RC6630, RC6640
The product is stable. Stability
10.
:
:
:
Toxicological Information 11.
Acute Effects
Eye Contact RC0960, RC1400, RC1920, RC6630 Moderately irritating to the eyes.
RC6640 Severely corrosive to the eyes.
RC1400, RC1920,
RC6630
Moderately irritating to the skin.
RC6640 Severely corrosive to the skin.
Skin Contact
RC6640 May cause damage to the following organs: lungs, gastrointestinal tract, upper respiratory
tract, head.
Target Organs
Carcinogenic Effects Not available.
Not available.
Developmental and
Teratogenic Effects
RC1890 Developmental toxicity: Classified Development toxin [PROVEN].
Mutagenic Effects
Reproductive Effects
Other Information
Not available.
Inhalation
Ingestion
RC0960 Repeated exposure to a highly toxic material may produce general deterioration of health
by an accumulation in one or many human organs.
RC1180, RC1400,
RC1890, RC1920,
RC6630
Repeated or prolonged exposure is not known to aggravate medical condition.
RC6640 Repeated exposure of the eyes to a low level of dust can produce eye irritation. Repeated
skin exposure can produce local skin destruction, or dermatitis. Repeated inhalation of
dust can produce varying degree of respiratory irritation or lung damage.
Ingredient Name
RC0960 LD50 30 mg/kg Oral Mouse
RC1180 Not available. Not available. Not available. Not available.
RC1400 LD50
LDLo
5900 mg/kg
1000 mg/kg
Oral
Oral
Rat
Rabbit
RC1890 Not available. Not available. Not available. Not available.
RC1920 Not available. Not available. Not available. Not available.
RC6630 Not available. Not available. Not available. Not available.
RC6640 Not available. Not available. Not available. Not available.
RC0960 Toxic if swallowed.
RC1400 Practically non-toxic if swallowed.
RC1920 Moderately irritating to the respiratory system.
RC6630 May cause sensitization by inhalation. Moderately irritating to the respiratory system.
RC6640 Severely corrosive to the respiratory system.
Species Route Result Test
Chronic Effects
Toxicity Data
:
:
:
:
:
:
:
:
Adverse Effects : RC6630 Adverse symptoms may include: sensitization.
RC6640 Adverse symptoms may include: respiratory tract irritation inflammation chemical
pneumonitis pulmonary edema coughing wheezing - breathing difficulties shortness of
breath/breathing difficulty headache nausea/vomiting death.
Routes of Entry Absorbed through skin. Dermal contact. Eye contact. Inhalation. Ingestion. :
:
:
Ecological Information 12.
RC0960 Not available. Not available. Not available.
RC1180 Not available. Not available. Not available.
RC1400 Not available. Not available. Not available.
RC1890 Not available. Not available. Not available.
RC1920 Not available. Not available. Not available.
RC6630 Not available. Not available. Not available.
RC6640 Not available. Not available. Not available.
Species Period Result
Ecotoxicity Data
Ingredient Name
Page: 5/5 Catalog # QIA21
Disposal Consideration 13.
Waste Handling and Disposal Waste must be disposed of in accordance with federal, state and local environmental control regulations. :
Transport Information 14.
Air
Proper shipping name Urea hydrogen peroxide :
UN/ID Number
IATA-DGR Class IATA Class 5.1: Oxidizing substance. IATA Class 8: Corrosive solid. :
IATA label :
: UN1511
Packing Group : III
Regulatory Information 15.
Safety Phrases S3/7- Keep container tightly closed in a cool place. S14.11- Keep away from flammable material. S24- Avoid
contact with skin. S26- In case of contact with eyes, rinse immediately with plenty of water and seek medical
advice. S36/37/39- Wear suitable protective clothing, gloves and eye/face protection. S45- In case of accident or if
you feel unwell, seek medical advice immediately (show the label where possible). S61- Avoid release to the
environment. Refer to special instructions/Safety data sheets.
R8- Contact with combustible material may cause fire. R34- Causes burns. R36/37/38- Irritating to eyes,
respiratory system and skin. R42- May cause sensitization by inhalation. R52/53- Harmful to aquatic organisms,
may cause long-term adverse effects in the aquatic environment.
:
:
:
Hazard Symbol(s)
Risk Phrases
EU Regulations
US Regulations
CLASS: Highly toxic.
CLASS: Target organ effects.
CLASS: Corrosive material
State California prop. 65: This product contains the following ingredients for which the State of California has found to
cause birth defects which would require a warning under the statute: Penicillin-Streptomycin
California prop. 65: This product contains the following ingredients for which the State of California has found to
cause cancer which would require a warning under the statute: 3,3'-Diaminobenzidine, 4HCl
TSCA 8(b) inventory: Tris Base
Haz-Com Standard
EPA
:
:
:
Canadian Regulations
WHMIS
Provincial :
:
: CLASS D-1A: Material causing immediate and serious toxic effects (VERY TOXIC).
CLASS D-2A: Material causing other toxic effects (VERY TOXIC).
CLASS E: Corrosive liquid.
CEPA DSL: Tris Base
No products were found.
CEPA
C; N; O; Xn
Other Information
12/23/2003.
16.
: Date of Printing
1.0 : Version
Notice to Reader
Validated by so2 on 12/23/2003.
To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries assumes any
liability whatsoever for the accuracy or completeness of the information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with
caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. **This product is intended for research
use only.**