Material Safety Data Sheet Ordering Products What's New Support Who We Are Services SEARCH HOME Français Catalogues Frequently Asked Questions Laboratory Vendor Links Safety Vendor Links Scratch and Dent Sales FisherFast Online - Order Approval Process now available! Laboratory Products Safety Products Services Contact Us Customer Service GJS Mass Measurement 1-877-627-7225 Instrument Service 1-800-267-6633 Mailing Addresses Corporate Profile Employment Global Links President's Message Quality Management Policy Certificates of Analysis Material Safety Data Sheets Site Search Product Search **** SECTION 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION **** MSDS Name: Methylene Blue Catalog Numbers: S71317, S71318, S71325, S71326, S80083, BP117 100, BP117-100, BP117100, M235 100, M291 100, M291 25, M291-100, M291-25, M291100, M29125 Synonyms: 3H-Phenothiazine, 7-(dimethylamino)-3-(methylimino)-, 3-methochloride; 3,7-Bis (dimethylamino) phenazathionium chloride; 3,7-(dimethylamino)phenothiazin-5-ium chloride; C.I. 52015; C.I. Basic Blue 9; Methylthionine; Methylthionine chloride; Methylthioniu Company Identification: Fisher Scientific 1 Reagent Lane Fairlawn, NJ 07410 For information, call: 201-796-7100 Emergency Number: 201-796-7100 For CHEMTREC assistance, call: 800-424-9300 For International CHEMTREC assistance, call: 703-527-3887 **** SECTION 2 - COMPOSITION, INFORMATION ON INGREDIENTS **** +----------------+--------------------------------------+----------+-----------+ | CAS# | Chemical Name | % | EINECS# | |----------------|--------------------------------------|----------|-----------| | 61-73-4 |Methylene blue | ca.100 | 200-515-2 | +----------------+--------------------------------------+----------+-----------+ Hazard Symbols: XN Risk Phrases: 22 **** SECTION 3 - HAZARDS IDENTIFICATION **** EMERGENCY OVERVIEW Harmful if swallowed.Mutagen. Potential Health Effects Eye: May cause chemical conjunctivitis. Causes eye irritation and possible injury. Skin: Causes skin irritation. Absorption into the body may cause cyanosis. Ingestion: May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause hemolytic anemia. May be harmful if swallowed. Inhalation: Causes respiratory tract irritation. May cause methemoglobinemia, cyanosis, convulsions, tachycardia, dyspnea, and death. Can produce delayed pulmonary edema. Chronic: Effects may be delayed. Laboratory experiments have resulted in mutagenic effects. Repeated contact may cause corneal damage. May cause adverse reproductive effects. **** SECTION 4 - FIRST AID MEASURES **** Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid. Skin: Get medical aid. Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid. Inhalation: Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid. Do NOT use mouth-to-mouth resuscitation. Notes to Physician: Treat symptomatically and supportively. **** SECTION 5 - FIRE FIGHTING MEASURES **** General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. This material in sufficient quantity and reduced particle size is capable of creating a dust explosion. Extinguishing Media: Use extinguishing media most appropriate for the surrounding fire. Use water spray, dry chemical, carbon dioxide, or appropriate foam. **** SECTION 6 - ACCIDENTAL RELEASE MEASURES **** General Information: Use proper personal protective equipment as indicated in Section 8. Spills/Leaks: Vacuum or sweep up material and place into a suitable disposal container. Clean up spills immediately, observing precautions in the Protective Equipment section. Avoid generating dusty conditions. Provide ventilation. **** SECTION 7 - HANDLING and STORAGE **** Handling: Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use only in a well-ventilated area. Minimize dust generation and accumulation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. Wash clothing before reuse. Storage: Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. **** SECTION 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION **** Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low. Personal Protective Equipment Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure. Respirators: A respiratory protection program that meets OSHA's 29 CFR :1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use. **** SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES **** Physical State: Solid Appearance: dark green Odor: odorless pH: Basic. Vapor Pressure: Negligible. Vapor Density: 13 Evaporation Rate: Not available. Viscosity: Not available. Boiling Point: Decomposes. Freezing/Melting Point: 190 deg C Autoignition Temperature: Not available. Flash Point: Not available. Explosion Limits, lower: Not available. Explosion Limits, upper: Not available. Decomposition Temperature: Not available. Solubility: Slightly soluble. Specific Gravity/Density: 1.230 Molecular Formula: C16H18ClN3S Molecular Weight: 319.6591 **** SECTION 10 - STABILITY AND REACTIVITY **** Chemical Stability: Stable at room temperature in closed containers under normal storage and handling conditions. Conditions to Avoid: Incompatible materials, dust generation, excess heat. Incompatibilities with Other Materials: Oxidizing agents, reducing agents, bases, dichromates, alkali iodides, caustic alkalis. Hazardous Decomposition Products: Hydrogen chloride, nitrogen oxides, carbon monoxide, irritating and toxic fumes and gases, carbon dioxide, sulfur oxides (SOx), including sulfur oxide and sulfur dioxide. Hazardous Polymerization: Will not occur. **** SECTION 11 - TOXICOLOGICAL INFORMATION **** RTECS#: CAS# 61-73-4: SO5600000 LD50/LC50: CAS# 61-73-4: Oral, mouse: LD50 = 3500 mg/kg; Oral, rat: LD50 = 1180 mg/kg. Carcinogenicity: Methylene blue - Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA. Epidemiology: No information available. Teratogenicity: No information available. Reproductive Effects: Oral, rat: TDLo = 2500 mg/kg (female 1-22 day(s) after conception) Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants). Neurotoxicity: No information available. Mutagenicity: DNA Adduct: Mouse, Ascites tumor = 70 umol/L.; DNA Adduct: Mammal - species unspecified Lymphocyte = 1830 nmol/L. Other Studies: No data available. **** SECTION 12 - ECOLOGICAL INFORMATION **** Ecotoxicity: Laboratory adsorption studies determined that methylene blue was strongly adsorbed to three different soils. Volatilization of methylene blue will not be important from moist or dry soil surfaces. If released to water, methylene blue would adsorb to suspended solids and sediment based upon soil adsorption studies. It will be essentially non-volatile from water surfaces. An estimated BCF value of 1.5 suggests that methylene blue will not bioconcentrate in aquatic organisms. **** SECTION 13 - DISPOSAL CONSIDERATIONS **** Dispose of in a manner consistent with federal, state, and local regulations. **** SECTION 14 - TRANSPORT INFORMATION **** US DOT No information available Canadian TDG No information available. **** SECTION 15 - REGULATORY INFORMATION **** US FEDERAL TSCA CAS# 61-73-4 is listed on the TSCA inventory. This material does not contain any Class 2 Ozone depletors. Clean Water Act: None of the chemicals in this product are listed as Hazardous Substances under the CWA. None of the chemicals in this product are listed as Priority Pollutants under the CWA. None of the chemicals in this product are listed as Toxic Pollutants under the CWA. OSHA: None of the chemicals in this product are considered highly hazardous by OSHA. STATE Methylene blue is not present on state lists from CA, PA, MN, MA, FL, or NJ. California No Significant Risk Level: None of the chemicals in this product are listed. European/International Regulations European Labeling in Accordance with EC Directives Hazard Symbols: XN Risk Phrases: R 22 Harmful if swallowed. Safety Phrases: S 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S 36/37/39 Wear suitable protective clothing, gloves and eye/face protection. WGK (Water Danger/Protection) CAS# 61-73-4: 2 United Kingdom Occupational Exposure Limits Canada CAS# 61-73-4 is listed on Canada's DSL List. CAS# 61-73-4 is listed on Canada's Ingredient Disclosure List. Exposure Limits **** SECTION 16 - ADDITIONAL INFORMATION **** MSDS Creation Date: 7/14/1999 Revision #9 Date: 4/06/2001 The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no way shall the company be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if the company has been advised of the possibility of such damages. --------------------------------------------------------------------------------