Eng 
Material Safety Data Sheet 
Conforms to 93/112/EC and ISO 11014-1 Responsible Name Pierce Administration 
1.800.874.3723 
Section 1. Chemical Product and Company Identification 
Manufacturer Pierce Biotechnology 
P.O. Box 117 
Rockford, IL 61105 
USA 
815.968.0747 or 
Print Date 11/13/2002 
Validation Date 11/4/2002 
MSDS# 1229 
R-Phrases Symbol EC Number 
R36/37/38 
R21/22, R36/37/38 
R36/37/38 
Xi 
Xn 
Xi 
Product name 1-Step NBT/BCIP 
Product No. 0034042 
Supplier In USA: 
9320 Erembodegem-Aalst 
Belgium 
Tel:+32 53 83 44 04 
Fax:+32 53 83 76 38 
In Europe: 
Pierce Perbio Science 
P.O. Box 117 Industriezone III 
Rockford, IL 61105 Industrielaan 27 
USA 
815.968.0747 or 
1.800.874.3723 
In Case of Emergency CALL CHEMTREC: 
800.424.9300 
OUTSIDE US: 
202.483.7616 
Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. 
Section 2. Composition, Information on Ingredients 
Substance/Preparation Preparation : 
CAS No. Ingredient Name % 
1) 1-Step NBT/BCIP Component 1 
2) 1-Step NBT/BCIP Component 2 
3) 1-Step NBT/BCIP Component 3 
0-0.1 
0.1-1 
1-3 
Section 3. Hazards Identification 
United States Review the most current and approved institutional guideline, protocol, standard operating procedure(s) and MSDS(s) 
for the proper handling of institutional materials/equipment associated with the use of this product. 
Emergency Overview WARNING! 
BIRTH DEFECT HAZARD 
CONTAINS MATERIAL WHICH CAN CAUSE BIRTH DEFECT. 
CAUSES DAMAGE TO THE FOLLOWING ORGANS: HEART, EYES. 
MAY CAUSE EYE AND SKIN IRRITATION. 
Avoid contact with eyes, skin and clothing. Avoid exposure during pregnancy. Wash thoroughly after handling. 
Eye contact. Ingestion. Routes of Entry 
Potential Acute Health Effects 
Carcinogenic Effects Data 
Slightly hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion. Non-hazardous in case of 
inhalation. Non-irritant for lungs. 
CARCINOGENIC EFFECTS: Classified None. by NIOSH [1-Step NBT/BCIP Component 2]. Classified A4 (Not 
classifiable for human or animal.) by ACGIH [1-Step NBT/BCIP Component 2]. 
MUTAGENIC EFFECTS: Not available. 
TERATOGENIC EFFECTS: Not available. 
Repeated or prolonged exposure is not known to aggravate medical condition. Medical Conditions Aggravated 
by Overexposure: 
Overexposure /Signs/Symptoms Not available. 
Europe 
Classification R36/38 
Not applicable. 
Irritating to eyes and skin. 
Physical/chemical hazards 
Human health hazards 
Environmental hazards Not applicable. 
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See Toxicological Information (section 11) 
Section 4. First Aid Measures 
Notice to Reader Get immediate medical attention. 
Effects and symptoms 
Inhalation Non-hazardous in case of inhalation. Non-irritant for lungs. 
Ingestion Slightly hazardous in case of ingestion. 
Skin Contact Sensitization of the product: Not available. 
Slightly hazardous in case of skin contact (irritant). Skin inflammation is characterized by itching, scaling, reddening, or, 
occasionally, blistering. 
Eye Contact Slightly hazardous in case of eye contact (irritant). 
Aggravating conditions Repeated or prolonged exposure is not known to aggravate medical condition. 
First-Aid Measures 
Inhalation If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical 
attention if symptoms appear. 
Ingestion Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious 
person. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention if symptoms appear. 
Skin Contact In case of contact, immediately flush skin with plenty of water. Cover the irritated skin with an emollient. Remove 
contaminated clothing and shoes. Cold water may be used.Wash clothing before reuse. Thoroughly clean shoes before 
reuse. Get medical attention. 
Eye Contact Check for and remove any contact lenses. Immediately flush eyes with running water for at least 15 minutes, keeping 
eyelids open. Cold water may be used. Get medical attention. 
Notes to Physician Not available. 
Protection of first-aiders Not available. 
Section 5. Fire Fighting Measures 
Flammability of the Product May be combustible at high temperature. 
Flash Points Not available. 
Fire Hazards in Presence of Various Non-flammable in presence of shocks, of oxidizing materials, of reducing materials, of combustible materials, of organic 
Substances materials, of metals, of acids, of alkalis. 
Fire Fighting Media SMALL FIRE: Use DRY chemical powder. 
and Instructions LARGE FIRE: Use water spray, fog or foam. Do not use water jet. 
Protective Clothing (Fire) Be sure to use an approved/certified respirator or equivalent. 
Special Remarks on Fire Hazards When heated to decomposition it emits acrid smoke and irritating fumes. (1-Step NBT/BCIP Component 2) 
Hazardous thermal (de)composition These products are carbon oxides (CO, CO2), nitrogen oxides (NO, NO2...). 
products 
Section 6. Accidental Release Measures 
Personal precautions Safety glasses. Lab coat. 
Environmental Precautions and Absorb with an inert material and put the spilled material in an appropriate waste disposal. Neutralize the residue with 
Clean-up Methods a dilute solution of acetic acid. Finish cleaning by spreading water on the contaminated surface and allow to evacuate 
through the sanitary system. Be careful that the product is not present at a concentration level above TLV. Check TLV 
on the MSDS and with local authorities. 
Small Spill and Leak Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container. 
If necessary: Neutralize the residue with a dilute solution of acetic acid. Finish cleaning by spreading water on the 
contaminated surface and dispose of according to local and regional authority requirements. 
Continued on Next Page
1-Step NBT/BCIP 
Section 7. Handling and Storage 
Handling Avoid contact with eyes, skin, and clothing. Wash thoroughly after handling. 
Storage Keep container tightly closed. Keep container in a cool, well-ventilated area. Store between 2 to 8°C (35.6 to 46.4°F). 
Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. 
Packaging materials 
Suitable / Not suitable Use original container. 
United States 
1) 1-Step NBT/BCIP Component 3 
Sweden 
1) 1-Step NBT/BCIP Component 1 
2) 1-Step NBT/BCIP Component 2 
3) 1-Step NBT/BCIP Component 3 
Denmark 
1) 1-Step NBT/BCIP Component 1 
2) 1-Step NBT/BCIP Component 2 
3) 1-Step NBT/BCIP Component 3 
Norway 
1) 1-Step NBT/BCIP Component 1 
2) 1-Step NBT/BCIP Component 2 
3) 1-Step NBT/BCIP Component 3 
France 
1) 1-Step NBT/BCIP Component 1 
2) 1-Step NBT/BCIP Component 2 
3) 1-Step NBT/BCIP Component 3 
Netherlands 
1) 1-Step NBT/BCIP Component 1 
2) 1-Step NBT/BCIP Component 2 
3) 1-Step NBT/BCIP Component 3 
Section 8. Exposure Controls/Personal Protection 
Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their 
respective threshold limit value. Ensure that eyewash stations and safety showers are proximal to the work-station 
location. 
Exposure Limit Values 
Ingredient Name Occupational Exposure Limits 
Not available. 
Not available. 
TWA: 10 STEL: 50 CEIL: 20 (ppm) from AFS [Sweden] [1996] SKIN 
Not available. 
Not available. 
TWA: 10 (ppm) from Arbejdstilsynet [Denmark] [1996] SKIN 
Not available. 
Not available. 
TWA: 10 CEIL: 125 (mg/m3) from Arbeidstilsynet [Norway] [1996] Inhalation 
Not available. 
Not available. 
STEL: 50 (ppm) from INRS [France] [1999] Inhalation 
Not available. 
Not available. 
TWA: 26 (mg/m3) from Arbeidsinspectie [Netherlands] [1999] Inhalation 
Not available. 
Not available. 
TWA: 10 CEIL: 10 (ppm) from BAuA [Germany] [1999] SKIN 
Not available. 
Germany 
1) 1-Step NBT/BCIP Component 1 
2) 1-Step NBT/BCIP Component 2 
3) 1-Step NBT/BCIP Component 3 
Personal Protection 
Eyes Splash goggles. 
Body Lab coat. 
Hands Gloves. 
Respiratory Respirator is not needed under normal and intended conditions of use, if exposures are kept below established limits. 
Protective Clothing 
(Pictograms) 
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Odor Not available. 
Taste Not available. 
Color Yellowish. Purple. 
Section 9. Physical and Chemical Properties 
Physical State and Liquid. 
Appearance 
Molecular Weight Not applicable. 
Basic. pH (1% Soln/Water) 
Boiling/Condensation Point 100°C (212°F) 
Melting/Freezing Point 1°C (33.8°F) 
Specific Gravity The only known value is 1 (Water = 1) (Milli-Q Water). 
Vapor Pressure The highest known value is 2.3 kPa (@ 20°C) (Milli-Q Water). 
Vapor Density The highest known value is 0.62 (Air = 1) (Milli-Q Water). 
Evaporation Rate 0.36 (Milli-Q Water) compared to Butyl acetate. 
LogKow The product is more soluble in water. 
Dispersion Properties See solubility in water, methanol, diethyl ether, acetone. 
Solubility Easily soluble in methanol, diethyl ether. 
Partially soluble in cold water, hot water, acetone. 
Very slightly soluble in n-octanol. 
Section 10. Stability and Reactivity 
Stability and Reactivity The product is stable. 
Conditions to avoid Not available. 
Materials to avoid Reactive with oxidizing agents. 
Slightly reactive to reactive with alkalis. 
Hazardous Polymerization Non-reactive with metals, acids. 
Will not occur. 
Hazardous Decomposition These products are carbon oxides (CO, CO2), nitrogen oxides (NO, NO2...). 
Products 
Section 11. Toxicological Information 
Toxicity to Animals Acute oral toxicity (LD50): 5900 mg/kg [Rat]. (1-Step NBT/BCIP Component 3). 
Chronic Effects on Humans CARCINOGENIC EFFECTS: Classified None. by NIOSH [1-Step NBT/BCIP Component 2]. Classified A4 (Not classifiable 
for human or animal.) by ACGIH [1-Step NBT/BCIP Component 2]. 
DEVELOPMENTAL TOXICITY: Classified Reproductive system/toxin/female, Reproductive system/toxin/male [PROVEN] 
[1-Step NBT/BCIP Component 2]. 
Causes damage to the following organs: heart, eyes. 
Other Toxic Effects on Humans Slightly hazardous in case of eye contact (irritant), of ingestion. 
Non-irritant for lungs. Non-sensitizer for lungs. 
Special Remarks on Toxicity to Not available. 
Animals 
Special Remarks on Chronic Not available. 
Effects on Humans 
Special Remarks on Other Toxic To the best of our knowledge, the toxicological properties of this product have not been thoroughly investigated. (1-Step 
Effects on Humans NBT/BCIP Component 3) 
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Section 12. Ecological Information 
Mobility 
Persistence/degradability 
Bioaccumulative potential 
Not available. 
Not available. 
Not available. 
Ecotoxicity Not available. 
Germany water class VCI WGK: No products were found. 
Section 13. Disposal Considerations 
Waste Information Waste must be disposed of in accordance with federal, state and local environmental control regulations. 
Waste Stream Not available. 
Consult your local or regional authorities. 
Section 14. Transport Information 
Contact the supplier for all information regarding the proper transportation method for this material. 
1 Section 15. Regulatory Information 
R36/38- Irritating to eyes and skin. 
Label Requirements 
(Europe) 
S26- In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. 
S28- After contact with skin, wash immediately with plenty of water. 
Irritant 
HCS Classification Irritating substance. 
U.S. Federal Regulations TSCA 8(b) inventory: 1-Step NBT/BCIP Component 1; 1-Step NBT/BCIP Component 2; 1-Step NBT/BCIP Component 3; Milli-Q Water 
TSCA 8(d) H and S data reporting: 1-Step NBT/BCIP Component 2 
SARA 302/304/311/312 extremely hazardous substances: No products were found. 
SARA 302/304 emergency planning and notification: No products were found. 
SARA 302/304/311/312 hazardous chemicals: 1-Step NBT/BCIP Component 2 
SARA 311/312 MSDS distribution - chemical inventory - hazard identification: 1-Step NBT/BCIP Component 2: immediate health hazard, delayed 
health hazard 
Clean Water Act (CWA) 307: No products were found. 
Clean Water Act (CWA) 311: No products were found. 
Clean air act (CAA) 112 accidental release prevention: No products were found. 
Clean air act (CAA) 112 regulated flammable substances: No products were found. 
Clean air act (CAA) 112 regulated toxic substances: No products were found. 
Continued on Next Page
CEPA DSL: 1-Step NBT/BCIP Component 2; 1-Step NBT/BCIP Component 3; Milli-Q Water 
Not available. EINECS 
Germany water class: 1-Step NBT/BCIP Component 1; 1-Step NBT/BCIP Component 2 
Korea (TCCL): 1-Step NBT/BCIP Component 2; 1-Step NBT/BCIP Component 3; Milli-Q Water 
Philippines (RA6969): 1-Step NBT/BCIP Component 1; 1-Step NBT/BCIP Component 2; 1-Step NBT/BCIP Component 3; Milli-Q Water 
Page: 6/6 1-Step NBT/BCIP 
WHMIS (Canada) CLASS D-2A: Material causing other toxic effects (VERY TOXIC). 
International Regulations 
DSCL (EEC) R36/38- Irritating to eyes and skin. 
International Lists Australia (NICNAS): 1-Step NBT/BCIP Component 1; 1-Step NBT/BCIP Component 2; 1-Step NBT/BCIP Component 3; Milli-Q Water 
State Regulations Pennsylvania RTK: 1-Step NBT/BCIP Component 2: (environmental hazard, generic environmental hazard) 
Massachusetts RTK: 1-Step NBT/BCIP Component 2 
New Jersey: 1-Step NBT/BCIP Component 2 
California prop. 65: No products were found. 
Section 16. Other Information 
Health Hazardous Material 
Information System 
Fire Hazard 
Reactivity 
Personal Protection 
(U.S.A.) 
References Not available. 
Full text of R-Phrases 
referenced under headings 2 and 3: 
History of Document Changes Any information changes since last document version are marked with a triangle symbol. 
Irritating to eyes, respiratory system and skin. 
Harmful in contact with skin and if swallowed. 
Irritating to eyes, respiratory system and skin. 
Irritating to eyes, respiratory system and skin. 
Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. 
1
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0
a 
Validated by Pierce Administration on 11/4/2002. 
Notice to Reader 
To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries assumes any 
liability whatsoever for the accuracy or completeness of the information contained herein. 
Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. 
Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. 
* 
Fire Hazard 
National Fire Protection 
Association (U.S.A.) 0 
Health Reactivity 0 1 
Specific Hazard 
Verified by Pierce Administration. Date of Previous Issue No Previous 
Validation 
Version 0.01 Printed 11/13/2002.