Material Safety Data Sheet **** SECTION 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION **** MSDS Name: Papanicolaou Stain OG-6 Catalog Numbers: 23245668, 23245669, 23245670, 56011, 57046A, 57065A Synonyms: None. Company Identification: Biochemical Sciences, Inc. 200 Commodore Drive Swedesboro, NJ 08085 For information, call: 800-524-0294 Emergency Number: 800-524-0294 For CHEMTREC assistance, call: 800-424-9300 For International CHEMTREC assistance, call: 703-527-3887 **** SECTION 2 - COMPOSITION, INFORMATION ON INGREDIENTS **** +----------------+--------------------------------------+----------+-----------+ | CAS# | Chemical Name | % | EINECS# | |----------------|--------------------------------------|----------|-----------| | 64-17-5 |Ethanol | 84 | 200-578-6 | |----------------|--------------------------------------|----------|-----------| | 67-56-1 |Methanol | 4.4 | 200-659-6 | |----------------|--------------------------------------|----------|-----------| | 1936-15-8 |Orange G | <1.0 | 217-705-6 | |----------------|--------------------------------------|----------|-----------| | 7732-18-5 |Deionized Water | Balance | 231-791-2 | |----------------|--------------------------------------|----------|-----------| | 12067-99-1 |Phosphotungstic Acid | <1.0 | 235-087-6 | +----------------+--------------------------------------+----------+-----------+ Hazard Symbols: T F Risk Phrases: 11 **** SECTION 3 - HAZARDS IDENTIFICATION **** EMERGENCY OVERVIEW Highly flammable. Potential Health Effects Eye: Causes severe eye irritation. May cause painful sensitization to light. May cause chemical conjunctivitis and corneal damage. Skin: Causes moderate skin irritation. May be absorbed through the skin in harmful amounts. May cause cyanosis of the extremities. Ingestion: May be fatal or cause blindness if swallowed. May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause systemic toxicity with acidosis. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure. Inhalation: Inhalation of high concentrations may cause central nervous system effects characterized by nausea, headache, dizziness, unconsciousness and coma. Causes respiratory tract irritation. May cause narcotic effects in high concentration. Vapors may cause dizziness or suffocation. Chronic: May cause reproductive and fetal effects. Laboratory experiments have resulted in mutagenic effects. Animal studies have reported the development of tumors. Prolonged exposure may cause liver, kidney, and heart damage. **** SECTION 4 - FIRST AID MEASURES **** Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid. Skin: Get medical aid. Flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately. Induce vomiting by giving one teaspoon of Syrup of Ipecac. Inhalation: Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid. Notes to Physician: Treat symptomatically and supportively. Persons with skin or eye disorders or liver, kidney, chronic respiratory diseases, or central and peripheral nervous sytem diseases may be at increased risk from exposure to this substance. Antidote: Ethanol may inhibit methanol metabolism. **** SECTION 5 - FIRE FIGHTING MEASURES **** General Information: Containers can build up pressure if exposed to heat and/or fire. As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. Vapors may form an explosive mixture with air. Vapors can travel to a source of ignition and flash back. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Will burn if involved in a fire. Flammable Liquid. Can release vapors that form explosive mixtures at temperatures above the flashpoint. Use water spray to keep fire-exposed containers cool. Containers may explode in the heat of a fire. Extinguishing Media: For small fires, use dry chemical, carbon dioxide, water spray or alcohol-resistant foam. For large fires, use water spray, fog, or alcohol-resistant foam. Water may be ineffective. Do NOT use straight streams of water. **** SECTION 6 - ACCIDENTAL RELEASE MEASURES **** General Information: Use proper personal protective equipment as indicated in Section 8. Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Remove all sources of ignition. Use a spark-proof tool. Provide ventilation. A vapor suppressing foam may be used to reduce vapors. **** SECTION 7 - HANDLING and STORAGE **** Handling: Wash thoroughly after handling. Use only in a well-ventilated area. Ground and bond containers when transferring material. Use spark-proof tools and explosion proof equipment. Avoid contact with eyes, skin, and clothing. Empty containers retain product residue, (liquid and/or vapor), and can be dangerous. Keep container tightly closed. Avoid contact with heat, sparks and flame. Do not ingest or inhale. Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or open flames. Storage: Keep away from heat, sparks, and flame. Keep away from sources of ignition. Store in a tightly closed container. Keep from contact with oxidizing materials. Store in a cool, dry, well-ventilated area away from incompatible substances. Flammables-area. Do not store near perchlorates, peroxides, chromic acid or nitric acid. **** SECTION 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION **** Engineering Controls: Use explosion-proof ventilation equipment. Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate general or local exhaust ventilation to keep airborne concentrations below the permissible exposure limits. Personal Protective Equipment Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure. Respirators: A respiratory protection program that meets OSHA's 29 CFR :1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use. **** SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES **** Physical State: Liquid Appearance: red Odor: pungent pH: Not available. Vapor Pressure: 40 mm Hg @ 19 deg C Vapor Density: 1.59 Evaporation Rate: Not available. Viscosity: Not available. Boiling Point: 95 deg C Freezing/Melting Point: Not available. Autoignition Temperature: Not applicable. Flash Point: 55 deg F ( 12.78 deg C) Explosion Limits, lower: 3.3 vol % Explosion Limits, upper: 1.9 vol % Decomposition Temperature: Solubility: Soluble. Specific Gravity/Density: 1 Molecular Formula: Molecular Weight: **** SECTION 10 - STABILITY AND REACTIVITY **** Chemical Stability: Stable under normal temperatures and pressures. Conditions to Avoid: High temperatures, incompatible materials, ignition sources, excess heat, oxidizers. Incompatibilities with Other Materials: Strong oxidizing agents, acids, alkali metals, ammonia, hydrazine, peroxides, sodium, acid anhydrides, calcium hypochlorite, chromyl chloride, nitrosyl perchlorate, bromine pentafluoride, perchloric acid, silver nitrate, mercuric nitrate, potassium-tert-butoxide, magnesium perchlorate, acid chlorides, platinum, uranium hexafluoride, silver oxide, iodine heptafluoride, acetyl bromide, disulfuryl difluoride, tetrachlorosilane + water, acetyl chloride, permanganic acid, ruthenium (VIII) oxide, uranyl perchlorate, potassium dioxide. Hazardous Decomposition Products: Carbon monoxide, oxides of nitrogen, irritating and toxic fumes and gases, carbon dioxide, oxides of carbon. Hazardous Polymerization: Will not occur. **** SECTION 11 - TOXICOLOGICAL INFORMATION **** RTECS#: CAS# 64-17-5: KQ6300000 CAS# 67-56-1: PC1400000 CAS# 1936-15-8: QJ6500000 CAS# 7732-18-5: ZC0110000 CAS# 12067-99-1: TH5650000 LD50/LC50: CAS# 64-17-5: Draize test, rabbit, eye: 500 mg Severe; Draize test, rabbit, eye: 500 mg/24H Mild; Draize test, rabbit, skin: 20 mg/24H Moderate; Inhalation, mouse: LC50 = 39 gm/m3/4H; Inhalation, rat: LC50 = 20000 ppm/10H; Oral, mouse: LD50 = 3450 mg/kg; Oral, rabbit: LD50 = 6300 mg/kg; Oral, rat: LD50 = 7060 mg/kg. CAS# 67-56-1: Draize test, rabbit, eye: 40 mg Moderate; Draize test, rabbit, eye: 100 mg/24H Moderate; Draize test, rabbit, skin: 20 mg/24H Moderate; Inhalation, rat: LC50 = 64000 ppm/4H; Oral, mouse: LD50 = 7300 mg/kg; Oral, rabbit: LD50 = 14200 mg/kg; Oral, rat: LD50 = 5628 mg/kg; Skin, rabbit: LD50 = 15800 mg/kg. CAS# 1936-15-8. CAS# 7732-18-5: Oral, rat: LD50 = >90 mL/kg. CAS# 12067-99-1: Oral, rat: LD50 = 3300 mg/kg. Carcinogenicity: Ethanol - ACGIH: A4 - Not Classifiable as a Human Carcinogen Methanol - Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA. Orange G - IARC: Group 3 carcinogen Deionized Water - Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA. Phosphotungstic Acid - Not listed by ACGIH, IARC, NIOSH, NTP, or OSHA. Epidemiology: Ethanol has been shown to produce fetotoxicity in the embryo or fetus of laboratory animals. Prenatal exposure to ethanol is associated with a distinct pattern of congenital malformations that have collecetively been termed the "fetal alcohol syndrome". Teratogenicity: CAS# 64-17-4: Oral, Human - woman: TDLo = 41 gm/kg (female 41 week(s) after conception) Effects on Newborn - Apgar score (human only) and Effects on Newborn - other neonatal measures or effects and Effects on Newborn - drug dependence. Reproductive Effects: CAS# 64-17-4: Intrauterine, Human - woman: TDLo = 200 mg/kg (female 5 day(s) pre-mating) Fertility - female fertility index (e.g. # females pregnant per # sperm positive females; # females pregnant per # females mated). Neurotoxicity: No information available. Mutagenicity: CAS# 64-17-4: DNA Inhibition: Human, Lymphocyte = 220 mmol/L.; Cytogenetic Analysis: Human, Lymphocyte = 1160 gm/L.; Cytogenetic Analysis: Human, Fibroblast = 12000 ppm.; Cytogenetic Analysis: Human, Leukocyte = 1 pph/72H (Continuous).; Sister Chromatid Exchange: Human, Lymphocyte = 500 ppm/72H (Continuous). Other Studies: Standard Draize Test(Skin, rabbit) = 20 mg/24H (Moderate) Standard Draize Test: Administration into the eye (rabbit) = 500 mg (Severe). **** SECTION 12 - ECOLOGICAL INFORMATION **** Ecotoxicity: Fish: Rainbow trout: LC50 = 12900-15300 mg/L; 96 Hr; Flow-through @ 24-24.3&CFish: Rainbow trout: LC50 = 11200 mg/L; 24 Hr; Fingerling (Unspecified)Bacteria: Phytobacterium phosphoreum: EC50 = 34900 mg/L; 5-30 min; Microtox testCAS# 64-17-4: When spilled on land it is apt to volatilize, biodegrade, and leach into the ground water, but no data on the rates of these processes could be found. Its fate in ground water is unknown. When released into water it will volatilize and probably biodegrade. It would not be expected to adsorb to sediment or bioconcentrate in fish. **** SECTION 13 - DISPOSAL CONSIDERATIONS **** Dispose of in a manner consistent with federal, state, and local regulations. **** SECTION 14 - TRANSPORT INFORMATION **** US DOT Shipping Name: ETHANOL SOLUTION Hazard Class: 3 UN Number: UN1170 Packing Group: II Canadian TDG No information available. **** SECTION 15 - REGULATORY INFORMATION **** US FEDERAL TSCA CAS# 64-17-5 is listed on the TSCA inventory. CAS# 67-56-1 is listed on the TSCA inventory. CAS# 1936-15-8 is listed on the TSCA inventory. CAS# 7732-18-5 is listed on the TSCA inventory. CAS# 12067-99-1 is listed on the TSCA inventory. This material does not contain any Class 2 Ozone depletors. Clean Water Act: None of the chemicals in this product are listed as Hazardous Substances under the CWA. None of the chemicals in this product are listed as Priority Pollutants under the CWA. None of the chemicals in this product are listed as Toxic Pollutants under the CWA. OSHA: None of the chemicals in this product are considered highly hazardous by OSHA. STATE Ethanol can be found on the following state right to know lists: California, New Jersey, Florida, Pennsylvania, Minnesota, Massachusetts. Methanol can be found on the following state right to know lists: California, New Jersey, Florida, Pennsylvania, Minnesota, Massachusetts. Orange G is not present on state lists from CA, PA, MN, MA, FL, or NJ. Deionized Water is not present on state lists from CA, PA, MN, MA, FL, or NJ. Phosphotungstic Acid can be found on the following state right to know lists: California, (listed as ** no name **), New Jersey, (listed as ** no name **), Florida, (listed as ** no name **), Pennsylvania, (listed as ** no name **), Minnesota, (listed as ** no name **), Massachusetts, (listed as ** no name **). WARNING: This product contains Ethanol, a chemical known to the state of California to cause birth defects or other reproductive harm. California No Significant Risk Level: None of the chemicals in this product are listed. European/International Regulations European Labeling in Accordance with EC Directives Hazard Symbols: T F Risk Phrases: R 11 Highly flammable. Safety Phrases: S 7 Keep container tightly closed. S 9 Keep container in a well-ventilated place. S 16 Keep away from sources of ignition - No smoking. S 33 Take precautionary measures against static discharges. WGK (Water Danger/Protection) CAS# 64-17-5: 0 CAS# 67-56-1: 1 CAS# 1936-15-8: No information available. CAS# 7732-18-5: No information available. CAS# 12067-99-1: No information available. United Kingdom Occupational Exposure Limits CAS# 64-17-5: OES-United Kingdom, TWA 1000 ppm TWA; 1920 mg/m3 TWA CAS# 67-56-1: OES-United Kingdom, TWA 200 ppm TWA; 266 mg/m3 TWA CAS# 67-56-1: OES-United Kingdom, STEL 250 ppm STEL; 333 mg/m3 STEL CAS# 67-56-1: OES-United Kingdom, STEL 250 ppm STEL; 333 mg/m3 STEL CAS# 12067-99-1: OES-United Kingdom, TWA (listed as ** undefined **): 1 mg/m3 TWA CAS# 12067-99-1: OES-United Kingdom, STEL (listed as ** undefined **): soluble: 3 mg/m3 STEL; insoluble: 10 mg/m3 STEL Canada CAS# 64-17-5 is listed on Canada's DSL List. CAS# 67-56-1 is listed on Canada's DSL List. CAS# 1936-15-8 is listed on Canada's DSL List. CAS# 7732-18-5 is listed on Canada's DSL List. CAS# 12067-99-1 is listed on Canada's DSL List. CAS# 64-17-5 is listed on Canada's Ingredient Disclosure List. CAS# 67-56-1 is listed on Canada's Ingredient Disclosure List. CAS# 1936-15-8 is listed on Canada's Ingredient Disclosure List. CAS# 7732-18-5 is not listed on Canada's Ingredient Disclosure List. CAS# 12067-99-1 is not listed on Canada's Ingredient Disclosure List. Exposure Limits CAS# 64-17-5: OEL-AUSTRALIA:TWA 1000 ppm (1900 mg/m3) OEL-BELGIUM:TWA 1000 ppm (1880 mg/m3) OEL-CZECHOSLOVAKIA:TWA 1000 mg/m3;STEL 5000 mg/m3 OEL-DENMARK:TWA 1000 ppm (1900 mg/m3) OEL-FINLAND:TWA 1000 ppm (1900 mg/m3);STEL 1250 ppm (2400 mg/m3) OEL-FRANCE:TWA 1000 ppm (1900 mg/m3);STEL 5000 pp OEL-GERMANY:TWA 1000 ppm (1900 mg/m3) OEL-HUNGARY:TWA 1000 mg/m3;STEL 3000 mg/m3 OEL-THE NETHERLANDS:TWA 1000 ppm (1900 mg/m3) OEL-THE PHILIPPINES:TWA 1000 ppm (1900 mg/m3) OEL-POLAND:TWA 1000 mg/m3 OEL-RUSSIA:STEL 1000 mg/m3 OEL-SWEDEN:TWA 1000 ppm (1900 mg/m3) OEL-SWITZERLAND:TWA 1000 ppm (1900 mg/m3) OEL-THAILAND:TWA 1000 ppm (1900 mg/m3) OEL-TURKEY:TWA 1000 ppm (1900 mg/m3) OEL-UNITED KINGDOM:TWA 1000 ppm (1900 mg/m3) JAN9 OEL IN BULGARIA, COLOMBIA, JORDAN, KOREA check ACGIH TLV OEL IN NEW ZEALAND, SINGAPORE, VIETNAM check ACGI TLV CAS# 67-56-1: OEL-ARAB Republic of Egypt:TWA 200 ppm (260 mg/m3);Skin CAS# 12067-99-1 (listed as ** undefined **): OEL-DENMARK:TWA 5 mg/m3 OEL-RUSSIA:STEL 2 mg/m3 OEL-SWEDEN:TWA 5 mg/m3 OEL-UNITED KINGDOM:TWA 5 mg/m3;STEL 10 mg/m3 JAN9 **** SECTION 16 - ADDITIONAL INFORMATION **** MSDS Creation Date: 7/30/1999 Revision #2 Date: 8/02/2000 The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no way shall the company be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if the company has been advised of the possibility of such damages. --------------------------------------------------------------------------------