1.
Catalog # 69078
In Case of
Emergency
Product name Perfect Protein™ HRP Western Blot Kit
Product and Company Identification
Material Safety Data Sheet
Call Chemtrec®
(800)424-9300 (within U.S.A.)
(703)527-3887 (outside U.S.A.)
Supplier Manufactured by EMD Biosciences, Inc.
441 Charmany Drive
Madison, WI 53719
(608)238-6110
(800)207-0144
FAX: (608)238-1388
P.O. Box 12087
La Jolla, CA 92039-2087
(858)450-5558
(800)854-3417
FAX: (858)453-3552
Novagen
Order
Number
Customer
Number
Composition and Information on Ingredients
Sodium Dodecyl Sulfate 151-21-3 RC032 Xn R22, R36/38
beta-Mercaptoethanol 60-24-2 RC097 T, N R22, R23/24, R34, R51/53
Tris Base 77-86-1 RC140 Xi R36/38
Ingredient Name CAS No. R-Phrases
2.
Product No. EU Symbol
Note: See section 8 for occupational exposure limits and section 11 for LC50/LD50 information.
3. Hazards Identification
Primary Hazards and Critical
Effects
: RC032 WARNING!
HARMFUL IF INHALED.
MAY BE HARMFUL IF SWALLOWED.
MAY CAUSE EYE AND SKIN IRRITATION.
Avoid contact with eyes, skin and clothing. Do not ingest. Avoid breathing dust. Keep
container closed. Use only with adequate ventilation. Wash thoroughly after handling.
RC097 DANGER!
CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS.
HARMFUL IF INHALED OR ABSORBED THROUGH SKIN.
TOXIC TO AQUATIC ORGANISMS.
MAY BE HARMFUL IF SWALLOWED.
COMBUSTIBLE LIQUID AND VAPOR.
VAPOR MAY CAUSE FIRE.
MAY BE HARMFUL TO ENVIRONMENT IF RELEASED IN LARGE AMOUNTS.
Keep away from heat, sparks and flame. Do not get in eyes, on skin or on clothing. Avoid
contact with eyes, skin and clothing. Do not ingest. Do not breathe vapor or mist. Keep
container closed. Use only with adequate ventilation. Wash thoroughly after handling.
Avoid contact of spilled material and runoff with soil and surface waterways.
RC140 CAUTION!
MAY CAUSE EYE AND SKIN IRRITATION.
Avoid contact with eyes, skin and clothing. Wash thoroughly after handling.
Environmental Hazards RC097 Toxic to aquatic organisms. May cause long-term adverse effects in the aquatic
environment.
RC032 Harmful if ingested.
Irritating to eyes and skin.
RC097 Harmful if ingested.
Toxic by inhalation and in contact with skin.
Causes burns.
RC140 Irritating to eyes and skin.
Human Health Hazards
Physical/Chemical hazards : Not applicable
:
:
Catalog # 69078 Page: 2/5
RC032, RC097 Do NOT induce vomiting unless directed to do so by medical personnel.
Never give anything by mouth to an unconscious person. If large quantities of
this material are swallowed, call a physician immediately.
RC140 Do NOT induce vomiting unless directed to do so by medical personnel.
Never give anything by mouth to an unconscious person. Get medical
attention if symptoms appear.
Eye Contact
Skin Contact
RC032, RC140 In case of contact, immediately flush eyes with plenty of water for at least 15
minutes. Get medical attention.
RC097 In case of contact, immediately flush eyes with plenty of water for at least 15
minutes. Get medical attention immediately.
RC032, RC140 In case of contact, immediately flush skin with plenty of water. Remove
contaminated clothing and shoes. Wash clothing before reuse. Thoroughly
clean shoes before reuse. Get medical attention.
RC097 In case of contact, immediately flush skin with plenty of water for at least 15
minutes while removing contaminated clothing and shoes. Wash clothing
before reuse. Thoroughly clean shoes before reuse. Get medical attention
immediately.
4. First Aid Measures
Ingestion
Inhalation RC032, RC097 If inhaled, remove to fresh air. If not breathing, give artificial respiration. If
breathing is difficult, give oxygen. Get medical attention immediately.
RC140 If inhaled, remove to fresh air. If not breathing, give artificial respiration. If
breathing is difficult, give oxygen. Get medical attention
:
:
:
:
Notes to Medical Doctor : Not available.
5. Fire-Fighting Measures
Fire fighters should wear positive pressure self-contained breathing apparatus (SCBA) and full turnout
gear.
Hazardous Decomposition
Products
Fire/Explosion Hazards
These products are carbon oxides (CO, CO2), nitrogen oxides (NO, NO2...), sulfur oxides (SO2, SO3...).
Some metallic oxides.
Combustible liquid and vapor. Vapor may cause fire.
Fire-Fighting Procedures
Use foam or all purpose dry chemicals to extinguish. This material is toxic to aquatic organisms. Fire
water contaminated with this material must be contained and prevented from being discharged to any
waterway, sewer or drain.
:
:
:
Extinguishing Media :
Environmental Precautions
and Clean-up Methods
Personal Precautions
If emergency personnel are unavailable, contain spilled material. For small spills add absorbent (soil may
be used in the absence of other suitable materials) and use a non-sparking or explosion proof means to
transfer material to a sealed, appropriate container for disposal. For large spills dike spilled material or
otherwise contain material to ensure runoff does not reach a waterway. Place spilled material in an
appropriate container for disposal. Minimize contact of spilled material with soils to prevent runoff to
surface waterways.
Immediately contact emergency personnel. Eliminate all ignition sources. Keep unnecessary personnel
away. Use suitable protective equipment (Section 8). Follow all fire fighting procedures (Section 5). Do
not touch or walk through spilled material.
6. Accidental Release Measures
Note: See section 1 for emergency contact information and section 13 for waste disposal.
:
:
Handling
Handling and Storage
Storage
7.
:
RC032 Avoid contact with eyes, skin and clothing. Do not ingest. Avoid breathing dust
Keep container closed. Use only with adequate ventilation. Wash thoroughly after
handling.
RC097 Keep away from heat, sparks and flame. Do not get in eyes, on skin or on clothing.
Avoid contact with eyes, skin and clothing. Do not ingest. Do not breathe vapor o
mist. Keep container closed. Use only with adequate ventilation. Wash thoroughly
after handling. Avoid contact of spilled material and runoff with soil and surface
waterways. To avoid fire, minimize ignition sources
RC140 Avoid contact with eyes, skin and clothing. Wash thoroughly after handling
:
:
Use original container.
Keep container in a cool, well-ventilated area. Keep container tightly closed and sealed until ready for use.
Avoid all possible sources of ignition (spark or flame).
Packaging Materials
Catalog # 69078 Page: 3/5
Engineering Controls RC032 Open handling must be limited. Use local exhaust ventilation or handle in a ventilated
enclosure.
RC097 Open handling is not permitted. All handling must be performed in a glove box or
other totally enclosed system.
RC140 No special containment is required. Local exhaust ventilation should be provided.
RC032 Not available.
RC097 Not available.
RC140 Not available.
Ingredient Name Occupational Exposure Limits
Occupational Exposure Limits
8. Exposure Controls and Personal Protection
:
Skin and Body
Personal Protective Equipment
RC032, RC097 Use an approved, properly fitted, powered-air purifying respirator or a respirator of
equivalent or greater protection.
RC140 Use an approved, properly fitted, HEPA filter cartridge respirator, or a respirator of
greater protection if there is the potential to exceed the exposure limit(s).
:
RC032 Disposable outer garments when there is the potential for contact with the material.
RC097 Disposable outer garments, or impervious garments of equal or greater protection.
RC140 Work uniform or laboratory coat.
:
Hands :
RC032, RC140 Safety glasses. Goggles, face shield, or other full-face protection if potential exists
for direct exposure to dust.
RC097 Safety glasses. Goggles, face shield, or other full-face protection if potential exists
for direct exposure to aerosols or splashes.
Eyes :
Respiratory System
RC032, RC140 Use latex gloves. Additional body garments should be used based upon the
task being performed (e.g., sleevelets, apron, gauntlets, disposable suits).
RC097 Use chemical resistant, impervious gloves. Additional body garments
should be used based upon the task being performed (e.g., sleevelets,
apron, gauntlets, disposable suits) to avoid exposed skin surfaces.
Appropriate techniques should be used to remove potentially contaminated
clothing.
Physical and Chemical Properties
69047: 1 x 50 ul S-protein HRP Conjugate
69959: 1 x 125 ul Perfect Protein Western Markers, containing Sodium Dodecyl Sulfate (RC032), beta-Mercaptoethanol (RC097) and Tris Base
(RC140)
Flash Point
beta-Mercaptoethanol (RC097): OPEN CUP: 68°C (154.4°F).
9.
Kit Components
Hazardous Decomposition
Products
Conditions and Materials to
Avoid
RC032 Reactive with oxidizing agents.
RC097 Reactive with oxidizing agents, acids.
RC140 Reactive with oxidizing agents, alkalis.
Stability and Reactivity
Not available.
RC032, RC097,
RC140
Stability The product is stable.
10.
:
:
:
11. Toxicological Information
Ingredient Name Test Result Route Species
Toxicity Data
Catalog # 69078 Page: 4/5
Acute Effects
Eye Contact RC032, RC140 Moderately irritating to the eyes.
RC097 Corrosive to the eyes.
RC032, RC140 Moderately irritating to the skin.
RC097 Corrosive to the skin. Toxic in contact with skin.
Skin Contact
Target Organs Not available.
Carcinogenic Effects RC032, RC097 Classified None. by NIOSH.
Not available.
Developmental and
Teratogenic Effects
Not available.
Mutagenic Effects
Reproductive Effects
Other Information
Not available.
Inhalation
Ingestion
RC032 Repeated exposure to a highly toxic material may produce general deterioration of
health by an accumulation in one or many human organs.
RC097 Repeated or prolonged contact with spray mist may produce chronic eye irritation
and severe skin irritation. Repeated or prolonged exposure to spray mist may
produce respiratory tract irritation leading to frequent attacks of bronchial infection.
Repeated exposure to a highly toxic material may produce general deterioration of
health by an accumulation in one or many human organs.
RC140 Repeated or prolonged exposure is not known to aggravate medical condition.
RC032 LD50
LDLo
LC50
1288 mg/kg
10000 mg/kg
>3900 mg/m3 (1
hours)
Oral
Dermal
Inhalation
Rat
Rabbit
Rat
RC097 LD50
LD50
LC50
244 mg/kg
190 mg/kg
13200 mg/m3 (4
hours)
Oral
Oral
Inhalation
Rat
Mouse
Mouse
RC140 LD50
LDLo
5900 mg/kg
1000 mg/kg
Oral
Oral
Rat
Rabbit
RC032, RC097 Harmful if swallowed.
RC140 Practically non-toxic if swallowed.
RC032 Toxic by inhalation.
RC097 Corrosive to the respiratory system. Toxic by inhalation.
Chronic Effects
:
:
:
:
:
:
:
:
Adverse Effects : Not available.
Routes of Entry : Absorbed through skin. Dermal contact. Eye contact. Inhalation. Ingestion
:
:
12. Ecological Information
RC032 Not available. Not available. Not available.
RC097 Not available. Not available. Not available.
RC140 Not available. Not available. Not available.
Species Period Result
Ecotoxicity Data
Ingredient Name
13. Disposal Consideration
Waste Handling and Disposal : Waste must be disposed of in accordance with federal, state and local environmental control regulations.
14. Transport Information
Air
IATA-DGR Class : Not controlled under IATA.
Packing Group
Catalog # 69078 Page: 5/5
15. Regulatory Information
Safety Phrases S26- In case of contact with eyes, rinse immediately with plenty of water and
seek medical advice.
S36/37/39- Wear suitable protective clothing, gloves and eye/face
protection.
S45- In case of accident or if you feel unwell, seek medical advice
immediately (show the label where possible).
S61- Avoid release to the environment. Refer to special instructions/Safety
data sheets.
R22- Harmful if swallowed.
R23/24- Toxic by inhalation and in contact with skin.
R34- Causes burns.
R51/53- Toxic to aquatic organisms, may cause long-term
adverse effects in the aquatic environment.
:
:
:
Hazard Symbol(s)
Risk Phrases
EU Regulations
US Regulations
CLASS: Toxic.
CLASS: Irritating substance.
CLASS: Combustible liquid having a flash point between 37.8°C (100°F) and 93.3°C (200°F).
State Pennsylvania RTK: beta-Mercaptoethanol: (generic environmental hazard)
Massachusetts RTK: beta-Mercaptoethanol
TSCA 8(b) inventory: Sodium Dodecyl Sulfate; beta-Mercaptoethanol; Tris Base
SARA 302/304/311/312 hazardous chemicals: Sodium Dodecyl Sulfate; beta-Mercaptoethanol
SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Sodium Dodecyl
Sulfate: immediate health hazard, delayed health hazard; beta-Mercaptoethanol: fire, immediate
health hazard, delayed health hazard
Haz-Com Standard
EPA
:
:
:
Canadian Regulations
WHMIS
Provincial :
:
: CLASS B-3: Combustible liquid with a flash point between 37.8°C (100°F) and 93.3°C (200°F).
CLASS D-1A: Material causing immediate and serious toxic effects (VERY TOXIC).
CEPA DSL: Sodium Dodecyl Sulfate; beta-Mercaptoethanol; Tris Base
No products were found.
CEPA
T; C; N
Other Information
4/17/2003.
16.
Date of Printing :
Version : 1.0
To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries
assumes any liability whatsoever for the accuracy or completeness of the information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used
with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.
Notice to Reader
Validated by so2 on 4/16/2003.