QUALITY BIOLOGICAL INC  
SDS Protein Gel Buffer (5X)
Tris-Glycine-SDS (5X)
(Molecular Biology Grade)
Catalog #: 351-084-100 500 ml
351-084-130 1000 ml (1.0 L)
Store at: 15 to 30°C
Shipped at: ambient temperature
Composition: Tris base 15.14 g/L, 0.125M; Glycine 93.84 g/L, 1.25M;
SDS 0.5% (w/v), 5.00 g/L
in Molecular Biology Grade (MBG) Water
DESCRIPTION
Quality Biological’s (QBI) SDS Protein Gel
Buffer (5X) is prepared from molecular biology
grade Tris base
[tris(hydroxymethyl)aminomethane] and
sodium dodecyl sulfate (SDS), and USP
Grade glycine using Quality Biological’s
Molecular Biology Grade (MBG) Water. The
final product is sterile filtered through a 0.1 µm
filter.
APPLICATIONS
SDS Protein Gel Buffer (5X) is used for
denaturing polyacrylamide gel electrophoresis
(SDS-PAGE) of proteins.
QUALITY CONTROL
General
All QBI products for Molecular Biology are
prepared according to standard published
protocols1, 2 or to formulations provided by
customers. In addition, all products are
subjected to a variety of quality control
procedures, including pH and conductivity
determinations, in order to validate that the
test product is within its specifications.
Product Specific Testing
SDS Protein Gel Buffer (5X) is routinely
tested for the absence of DNase and RNase
activity. Protocols are shown on the next
page.
REFERENCES
1. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989)
Molecular Cloning, A Laboratory Manual, 2nd
Edition. Cold Spring Harbor Laboratory Press.
2. Ausubel, F.M. et al., eds. (1993)
Current Protocols in Molecular Biology.
Greene Publishing Associates, Inc., in
association with John Wiley & Sons, Inc.
3. Davis, L.G., Dibner, M.D. & Battey, J.F. (1986)
Basic Methods in Molecular Biology.
Elsevier Science Publishing Company, Inc.
Product Specific Testing Protocols
Deoxyribonuclease (DNase) Activity Testing
1. SDS Protein Gel Buffer (5X) is incubated at 37°C
with 1.0 µg of pBR322 and Pst I-digested fX174
DNA for 16-20 hours.
2. Subsequently, the test nucleic acids are
subjected to agarose gel electrophoresis and
SYBR® Green I staining.
3. The test DNA is evaluated relative to the
untreated DNA (negative control) for degradation
and changes in fragment size and/or banding
pattern which are both indicative of DNase
activity.
Ribonuclease (RNase) Activity Testing
1. SDS Protein Gel Buffer (5X) is incubated at 37°C
with prokaryotic MS2 ribosomal and eukaryotic
18S/28S ribosomal RNA substrates for 4 hours.
2. The test RNA is evaluated by non-denaturing
agarose gel electrophoresis and SYBR® Green II
staining.
3. RNA degradation is evidenced by broadening
and smearing of the RNA banding pattern.
RELATED PRODUCTS
Nondenaturing Protein Gel Buffer
Tris-Glycine (5X)
Catalog # 351-083-100 500 ml
351-083-130 1000 ml (1.0 L)
SDS Protein Gel Loading Solution (2X)
Catalog # 351-082-030 10 ml
Transblot Buffer (10X)
Catalog # 351-087-100 500 ml
Blocking Solution
Catalog # 351-080-101 500 ml (plastic)
All products sold by Quality Biological are
intended for research use only. This product
has not been approved for diagnostic or drug
use.
4/15/988