MATERIAL SAFETY DATA SHEET
ROI NUMBER 9657T
SYNONYMS Tris-EDTA Buffer Solution, 1X, pH 8.0, sterile,
Molecular Biology Grade
Each bottle contains: Tris 10mM, EDTA, disodium
1mM
TRADE NAME TE BUFFER SOLUTION, 1X, pH 8.0, STERILE, MOLECULAR
BIOLOGY GRADE
CAS NUMBER MIXTURE
Research Organics
4353 E 49th Street
Cleveland, Ohio
44125 USA
MANUFACTURER/
SUPPLIER
TELEPHONE NUMBERS - 24 HOUR EMERGENCY ASSISTANCE:
TELEPHONE NUMBERS - GENERAL ASSISTANCE:
INFOTRAC 800-535-5053
Product Information 800-321-0570
3 HAZARDS IDENTIFICATION
2 COMPOSITION / INFORMATION ON INGREDIENTS
1 CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
Ingredient Percentage* CAS Number Exposure Limits/Health Hazards
Tris (hydroxymethyl) Aminomethane <1 % 77-86-1 No Exposure limits established by OSHA or
ACGIH
IRRITANT. MAY BE HARMFUL.
EDTA, Disodium Salt, Dihydrate <1 % 6381-92-6 No Exposure limits established by OSHA or
ACGIH
IRRITANT. MAY BE HARMFUL.
Deionized Water >99 % 7732-18-5 No Exposure limits established by OSHA or
ACGIH
NO KNOWN HAZARD
Concentrated Hydrochloric Acid <1 % 7647-01-0 OSHA PEL CEILING: 5 ppm; 7 mg/m3.
ACGIH CEILING: 5 ppm; 7.5 mg/m3
%
CORROSIVE.
*Values do not reflect absolute minimums and maximums; those values may vary from time to time.
Emergency Overview
CAUTION!
MAY BE IRRITATING TO THE SKIN, EYES, AND UPPER RESPIRATORY TRACT.
MAY BE HARMFUL IF SWALLOWED, INHALED OR ABSORBED THROUGH SKIN
3/2/2006 Page 1 of 7 Effective Date:
Catalog Number 9657T Trade Name TE BUFFER SOLUTION, 1X, pH 8.0, STERILE, MOLECULAR BIOL
ND = No Data NA = Not Applicable Replaces Sheet Dated 9/8/2003
4 FIRST AID MEASURES
5 FIRE FIGHTING MEASURES
Potential Health Effects Skin
This material May cause skin irritation. Reddening, itching and inflammation may result.
May be absorbed through the skin.
Potential Health Effect Eyes
Direct contact may cause irritation, redness, tearing and blurred vision.
Potential Health Effects Inhalation
May cause irritation to the respiratory tract.
This material may cause injury or illness if inhaled.
Potential Health Effect Ingestion
If ingested, this material may cause irritation to the mouth, throat and gastrointestinal tract.
May be harmful if swallowed.
Special Toxic Effects
CAUTION! THE TOXICOLOGICAL PROPERTIES OF THIS MIXTURE HAVE NOT BEEN FULLY
INVESTIGATED.
Skin
Immediately flush skin with plenty of water, for at least 15 minutes, while removing contaminated clothing and
shoes. Contaminated clothing must be laundered before reuse. Get medical attention if irritation develops or
persists.
Eyes
Get medical attention if irritation persists.
Flush immediately with large amounts of water for at least 20 minutes. Eyelids should be held away from the
eyeball to ensure thorough rinsing. Do not allow victim to rub the affected eye(s).
Inhalation
Get medical attention if cough or other respiratory symptoms develop.
Safely remove victim to fresh air. If not breathing, institute artificial respiration/rescue breathing. If breathing is
difficult, ensure clear airway and give oxygen.
Ingestion
Never give anything by mouth to an unconscious person.
If swallowed, wash out mouth with water provided person is conscious.
Get medical attention if symptoms develop, worsen, or do not improve.
Hazardous Combustion Products
MAY EMIT TOXIC AND/OR IRRITATING FUMES UNDER FIRE CONDITIONS.
Extinguishing Media
Use a water spray, dry chemical, alcohol foam, all purpose foam or carbon dioxide to extinguish fire.
Basic Fire Fighting Procedure
As in any fire, wear self-contained breathing apparatus pressure-demand, (MSHA/NIOSH approved or
equivalent) and full protective gear. Evacuate the area and fight fire from a safe distance.
Target Organs
ND
3/2/2006 Page 2 of 7 Effective Date:
Catalog Number 9657T Trade Name TE BUFFER SOLUTION, 1X, pH 8.0, STERILE, MOLECULAR BIOL
ND = No Data NA = Not Applicable Replaces Sheet Dated 9/8/2003
6 ACCIDENTAL RELEASE MEASURES
7 HANDLING AND STORAGE
8 EXPOSURE CONTROLS / PERSONAL PROTECTION
Unusual Fire and Explosion Hazards
None known to manufacturer.
Emergency Action
Keep unnecessary people away; isolate hazard area and deny entry. Stay upwind. (See Personal Protection
Information Section.)
Environmental Precautions
If product is released to the environment, take immediate steps to stop the release. Caution should be
exercised regarding personnel safety and exposure to the released product. Notify local authorities and the
National Response Center, if required.
Spill or Leak Procedure
Absorb spill with inert material (e. g. dry sand or earth) then place in a chemical waste container. Clean up
spills immediately under quailfied supervision observing precautions in Protective Equipment section.
After removal, scrub the contaminated area with detergent and flush thoroughly with water. Absorb wash liquid
and place in the disposable container. Do not flush to sewer. Avoid runoff into storm sewers and ditches which
lead to waterways.
Handling
DO NOT GET IN EYES, ON SKIN OR CLOTHING.
Wash hands thoroughly before eating, drinking or smoking.
Storage
Store at room temperature.
Keep containers tightly closed and upright when not in use.
Engineering Contols
Facilities using or storing this material must be equipped with an eyewash station and safety shower.
Local exhaust ventilation, process enclosures, and other forms of engineering controls are the preferred means
for controlling exposures.
Eye Protection PPE
Do not wear contact lenses when working with this substance.
Avoid eye contact with this material. Wear chemical safety goggles.
Skin Protection PPE
Avoid skin contact with this material. Use appropriate chemical protective gloves when handling.
Showering and changing into street clothing after work is recommended.
Respiratory Protection PPE
A respiratory protection program that meets OSHA 1910.134 and ANSI Z88.2 requirements must be followed
whenever workplace conditions warrant a respirators use.
ND
ND
ND
ND
Flash Point Temperature
Autoignition Temperature
Flammability Limits in Air Lower
Flammability Limits in Air Upper
Storage Hazards
Store away from oxidizers.
3/2/2006 Page 3 of 7 Effective Date:
Catalog Number 9657T Trade Name TE BUFFER SOLUTION, 1X, pH 8.0, STERILE, MOLECULAR BIOL
ND = No Data NA = Not Applicable Replaces Sheet Dated 9/8/2003
9 PHYSICAL AND CHEMICAL PROPERTIES
10 STABILITY / REACTIVITY
11 TOXICOLOGICAL INFORMATION
Use process enclosures, local exhaust ventilation, or other engineering controls to control airborne levels below
recommended exposure limits.
A NIOSH/MSHA approved air purifying respirator is recommended where airborne concentrations are expected
to exceed exposure limits. Protection provided by air purifying respirators is limited. Air purifying respirators do
not provide oxygen.
Contact the manufacturer of your respiratory protection equipment for further information and/or
recommendations.
Appearance
Liquid.
ND
ND
ND
ND
ND
Boiling Point
Melting Point
Freezing Point
Vapor Density
Vapor Pressure
ND
ND
ND
7.8 - 8.2
Specific Gravity
Molecular Formula
Molecular Weight
pH
Stability/Incompatibility
Stable under normal conditions of use.
Incompatible with strong oxidizing agents.
Decomposition Products
Combustion may produce CO, CO2, and/or NOx.
Hazardous Polymerization
Will not occur.
Carcinogenicity
Information cited for: Hydrochloric Acid - IARC Cancer Review: Group 3 (Not classifiable as to Carcinogenicity in
humans).
Routes of Exposure
Inhalation, ingestion, skin and eye contact.
LD50
REGISTRY OF THE TOXIC EFFECTS OF CHEMICAL SUBSTANCES(RTECS)
RTECS # Mixture Not Listed.
NO ORAL LD50 IN RATS INFORMATION IS AVAILABLE FOR THIS MIXTURE.
Information cited for: Tris (hydroxymethyl) Aminomethane: RTECS # TY2900000. Oral rat LD50 - 5900 mg/kg.
Information cited for: EDTA, Disodium Salt, Dihydrate: RTECS # AH4410000.
Information cited for: Concentrated Hydrochloric Acid: RTECS# MW4025000.
Information cited for: Deionized Water: RTECS# ZC0110000.
Only selected data is presented here. See actual entry in RTECS for complete information.
CAUTION! THE TOXICOLOGICAL PROPERTIES OF THIS MIXTURE HAVE NOT BEEN FULLY
INVESTIGATED. FOLLOW GOOD LABORATORY PRACTICES WHEN HANDLING.
3/2/2006 Page 4 of 7 Effective Date:
Catalog Number 9657T Trade Name TE BUFFER SOLUTION, 1X, pH 8.0, STERILE, MOLECULAR BIOL
ND = No Data NA = Not Applicable Replaces Sheet Dated 9/8/2003
12 ECOLOGICAL INFORMATION
13 DISPOSAL CONSIDERATIONS
14 TRANSPORTATION INFORMATION
Department of Transportation (DOT) Requirements
Proper Shipping Name
Hazard Class
Packaging Group
Reportable Quantity
Labels Required
UN/NA Code
15 REGULATORY INFORMATION
Marine Pollutant (49 CFR 172.101 Appendix B)
EcoToxicological Information
DATA NOT YET AVAILABLE.
Chemical Fate Information
DATA NOT YET AVAILABLE.
Waste Disposal
Under RCRA, it is the responsibility of the user of the product to determine, at the time of disposal, whether the
product meets RCRA criteria for hazardous waste.
If the material is determined to be a hazardous waste, the transportation, storage, treatment and disposal of this
waste material must be conducted in compliance with 40 CFR 262, 263, 264, 268 and 270. Disposal can occur
only in properly permitted facilities.
Contact your local disposal service provider(s) for appropriate method(s) of disposal.
NOT REGULATED
NA
NA
None
NA
NA
Federal Regulations
This product is NOT on the EPA Toxic Substances Control Act (TSCA) inventory. The following
notices are required by 40 CFR 720.36 (C) for those products NOT on the inventory list: (i) This
product is supplied solely for use in Research and Development by
or under the supervision of a technically qualified individual as defined by 40 CFR 720.0 et.sec.
(ii) The health risks of this product have not fully been determined. Any information that is or
becomes available will be supplied on a Material Safety
Data Sheet. Uses other than for Research and Development may constitute a violation of TSCA.
INFORMATION CITED FOR: Tris (hydroxymethyl) Aminomethane: Listed on TSCA, section 8(b).
INFORMATION CITED FOR: Concentrated Hydrochloric Acid:
Listed on TSCA, sections 8(b), 8(d), and 8(e).
SARA EHS RQ: 5,000 pounds. SARA EHS TPQ: 500 pounds. SARA TR Concentr: 1.0%.
SARA Threshold: Standard.
Clean Air Act Data: HAP Code: X. Accidental Release Prevention: Threshold QTY: 5,000
pounds. Basis for listing: a.
INFORMATION CITED FOR: Deionized Water: Listed on TSCA.
State Regulations
Based on available information this material does not contain any components or
chemicals currently known to the State of California to cause cancer, birth defects or
reproductive harm at levels which would be subject to Proposition 65.
Reformulation, use or processing of this product may affect its composition and would
require re-evaluation
No
3/2/2006 Page 5 of 7 Effective Date:
Catalog Number 9657T Trade Name TE BUFFER SOLUTION, 1X, pH 8.0, STERILE, MOLECULAR BIOL
ND = No Data NA = Not Applicable Replaces Sheet Dated 9/8/2003
16 OTHER INFORMATION
NFPA is a registrated trademark of National Fire Protection Association. HMIS is a registrated trademark of National Paint and Coating Association.
Disclaimer
International Regulations
No Data for Mixture.
Information cited for: Tris (hydroxymethyl) Aminomethane: EINECS# 201-064-4.
Information cited for: Concentrated Hydrochloric Acid: EINECS# 231-595-7.
R - ND
S - 36/37/39
EINECS - ND
International Phrases
Not Determined
38
Wear suitable protective clothing, gloves & eye/face protection.
In case of insufficient ventilation, wear suitable respiratory equipment.
SARA TITLE III RATINGS
Immediate
Hazard
Delayed
Hazard
Fire
Hazard
Pressure
Hazard
Reactivity
Hazard
If this box is checked, this product contains EPCRA 313 chemical(s) subject to the reporting
requirements of Section 313 of the Emergency Planning and Community Right-To-Know Act of 1986
(40 CFR 372). If you are unsure whether you are subject to reporting requirements or need more
information, call EPA's ECPRA Call Center at (800) 424-9346 or (703) 412-9810.
Section 313 Supplier Notification:
NFPA(R) Ratings
Health Flammability Reactivity Special Hazards
- - - -
HMIS(R) RATINGS
Health Flammabilty Physical
Hazard
Personal Protection
Equipment
1 0 0 B+n
3/2/2006 Page 6 of 7 Effective Date:
Catalog Number 9657T Trade Name TE BUFFER SOLUTION, 1X, pH 8.0, STERILE, MOLECULAR BIOL
ND = No Data NA = Not Applicable Replaces Sheet Dated 9/8/2003
The data contained in this Material Safety Data Sheet relates only to the specific material
designated herein. It does not relate to use in combination with any other material or in any
process. Research Organics, Inc. provides the information contained herein in good faith but
make no representation as to its comprehensiveness or accuracy. Individuals receiving this
information must exercise their independent judgment in determining it appropriateness for a
particular purpose. Research Organics, Inc. makes no representations, or warranties, either
expressed or implied, of merchantability, fitness for a particular purpose with respect to the
information refers. Accordingly, Research Organics, Inc. will not be responsible for damages
resulting from the use of reliance upon this information. In no instance is any Research
Organics, Inc. product offered for drug, food, food additive or clinical use in humans.
Completed By: Environmental and Safety Department
Completed On: 3/2/2006 Replaces Sheet Dated: 9/8/2003
This material is sold for research and development only. Use in any applications other than
research and development may require prior approval from the EPA or inclusion on the TSCA
inventory.
3/2/2006 Page 7 of 7 Effective Date:
Catalog Number 9657T Trade Name TE BUFFER SOLUTION, 1X, pH 8.0, STERILE, MOLECULAR BIOL
ND = No Data NA = Not Applicable Replaces Sheet Dated 9/8/2003