Sigma - M5655
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SIGMA-ALDRICH
Material Safety Data Sheet
Version 3.0
Revision Date 07/13/2007
Print Date 11/02/2007
1. PRODUCT AND COMPANY IDENTIFICATION
Product name : Thiazolyl Blue Tetrazolium Bromide
Product Number : M5655
Brand : Sigma
Company : Sigma-Aldrich
3050 Spruce Street
SAINT LOUIS MO 63103
USA
Telephone : +1 800-325-5832
Fax : +1 800-325-5052
Emergency Phone # : (314) 776-6555
2. COMPOSITION/INFORMATION ON INGREDIENTS
Synonyms : 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium
bromide
MTT
Methylthiazolyldiphenyl-tetrazolium bromide
Formula : C18H16N5S·Br
Molecular Weight : 414.33 g/mol
CAS-No. EC-No. Index-No. Concentration [%]
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
298-93-1 206-069-5 - -
3. HAZARDS IDENTIFICATION
Emergency Overview
OSHA Hazards
No OSHA Hazards
HMIS Classification
Health Hazard: 0
Flammability: 0
Physical hazards: 0
NFPA Rating
Health Hazard: 0
Fire : 0
Reactivity Hazard: 0
Potential Health Effects
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Inhalation May be harmful if inhaled. May cause respiratory tract irritation.
Skin May be harmful if absorbed through skin. May cause skin irritation.
Eyes May cause eye irritation.
Ingestion May be harmful if swallowed.
4. FIRST AID MEASURES
If inhaled
If breathed in, move person into fresh air. If not breathing give artificial respiration
In case of skin contact
Wash off with soap and plenty of water.
In case of eye contact
Flush eyes with water as a precaution.
If swallowed
Never give anything by mouth to an unconscious person. Rinse mouth with water.
5. FIRE-FIGHTING MEASURES
Flammable properties
Flash point no data available
Ignition temperature no data available
Suitable extinguishing media
Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.
Special protective equipment for fire-fighters
Wear self contained breathing apparatus for fire fighting if necessary.
6. ACCIDENTAL RELEASE MEASURES
Personal precautions
Avoid dust formation.
Environmental precautions
Do not let product enter drains.
Methods for cleaning up
Sweep up and shovel. Keep in suitable, closed containers for disposal.
7. HANDLING AND STORAGE
Handling
Provide appropriate exhaust ventilation at places where dust is formed. Normal measures for preventive fire
protection.
Storage
Keep container tightly closed in a dry and well-ventilated place.
hygroscopic
8. EXPOSURE CONTROLS / PERSONAL PROTECTION
Contains no substances with occupational exposure limit values.
Personal protective equipment
Respiratory protection
Respiratory protection is not required. Where protection from nuisance levels of dusts are desired, use type N95
(US) or type P1 (EN 143) dust masks. Use respirators and components tested and approved under appropriate
government standards such as NIOSH (US) or CEN (EU).
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Hand protection
For prolonged or repeated contact use protective gloves.
Eye protection
Safety glasses
Hygiene measures
General industrial hygiene practice.
9. PHYSICAL AND CHEMICAL PROPERTIES
Appearance
Form solid
Colour dark yellow
Safety data
pH no data available
Melting point 195 °C (383 °F)
Boiling point no data available
Flash point no data available
Ignition temperature no data available
Lower explosion limit no data available
Upper explosion limit no data available
Water solubility no data available
10. STABILITY AND REACTIVITY
Storage stability
Stable under recommended storage conditions.
Conditions to avoid
Exposure to moisture may affect product quality.
Materials to avoid
Strong oxidizing agents
Hazardous decomposition products
Hazardous decomposition products formed under fire conditions.
Carbon oxides, nitrogen oxides (NOx), Hydrogen bromide gas, Sulphur oxides
11. TOXICOLOGICAL INFORMATION
Acute toxicity
no data available
Irritation and corrosion
no data available
Sensitisation
no data available
Chronic exposure
no data available
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Signs and Symptoms of Exposure
To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly
investigated.
Potential Health Effects
Inhalation May be harmful if inhaled. May cause respiratory tract irritation.
Skin May be harmful if absorbed through skin. May cause skin irritation.
Eyes May cause eye irritation.
Ingestion May be harmful if swallowed.
12. ECOLOGICAL INFORMATION
Elimination information (persistence and degradability)
no data available
Ecotoxicity effects
no data available
Further information on ecology
no data available
13. DISPOSAL CONSIDERATIONS
Product
Observe all federal, state, and local environmental regulations.
Contaminated packaging
Dispose of as unused product.
14. TRANSPORT INFORMATION
DOT (US)
Not dangerous goods
IMDG
Not dangerous goods
IATA
Not dangerous goods
15. REGULATORY INFORMATION
OSHA Hazards
No OSHA Hazards
TSCA Status
On TSCA Inventory
DSL Status
This product contains the following components listed on the Canadian NDSL list. All other components are on the
Canadian DSL list.
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
CAS-No.
298-93-1
SARA 302 Components
SARA 302: No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.
SARA 313 Components
SARA 313: This material does not contain any chemical components with known CAS numbers that exceed the
threshold (De Minimis) reporting levels established by SARA Title III, Section 313.
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SARA 311/312 Hazards
No SARA Hazards
Massachusetts Right To Know Components
No Components Listed
Pennsylvania Right To Know Components
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
CAS-No.
298-93-1
Revision Date
New Jersey Right To Know Components
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
CAS-No.
298-93-1
Revision Date
California Prop. 65 Components
This product does not contain any chemicals known to State of California to cause cancer, birth, or any other
reproductive defects.
16. OTHER INFORMATION
Further information
Copyright 2007 Sigma-Aldrich Co. License granted to make unlimited paper copies for internal use only., The above
information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our knowledge and is applicable to the product with
regard to appropriate safety precautions. It does not represent any guarantee of the properties of the product. Sigma-
Aldrich Co., shall not be held liable for any damage resulting from handling or from contact with the above product.
See reverse side of invoice or packing slip for additional terms and conditions of sale.