Kit Components 01/17/2008
Product code Description
1708230 Western Blot Amplification Module Kit
1708230EDU
Components:
9703204 Bio-Rad Amplification Reagent
9703205 2X Amplification Diluent
9703206 Streptavidin-HRP
9703207 Blocking Reagent
9703261 Phosphate Buffered Saline
Page 1/5
Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
DR
* 1 Identification of substance:
· Product details:
· Trade name: Bio-Rad Amplification Reagent
· Catalog or product number: 9703204
· Application of the substance / the preparation Laboratory chemicals
· Manufacturer/Supplier:
Bio-Rad Laboratories, Life Science Group
2000 Alfred Nobel Drive
Hercules, California 94547 (510)741-1000
· Information department: Technical services, customer support.
· Emergency information:
1(800)424-9300 Use only in the event of a CHEMICAL EMERGENCY involving a SPILL, LEAK, FIRE, EXPLOSION or ACCIDENT.
510-741-1000
* 2 Composition/Information on components:
· Chemical characterization:
· CAS No. Description:
7732-18-5 water
· Identification number(s):
· EINECS Number: 231-791-2
· Additional information: For the wording of the listed risk phrases refer to section 16.
* 3 Hazards identification
· Emergency overview: not applicable
· Information pertaining to particular dangers for man and environment not applicable
· Classification system
The classification was made according to OSHA 29CFR 1910.1200 and 1910.1030, and is expanded upon from company and/or
literature information.
4 First aid measures
· General information No special measures required.
· After inhalation Supply fresh air; consult doctor in case of complaints.
· After skin contact Generally the product does not irritate the skin.
· After eye contact Rinse opened eye for several minutes under running water.
· After swallowing Induce vomiting and call for medical help.
5 Fire fighting measures
· Suitable extinguishing agents CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
· Protective equipment: No special measures required.
6 Accidental release measures
· Person-related safety precautions: Not required
· Measures for environmental protection: No special measures required.
(Contd. on page 2)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: Bio-Rad Amplification Reagent
(Contd. of page 1)
DR
· Measures for cleaning/collecting: Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
· Additional information: No dangerous substances are released.
* 7 Handling and storage
· Handling
· Information for safe handling:
No special measures required.
No special precautions are necessary if used correctly.
· Information about protection against explosions and fires: No special measures required.
· Storage
· Requirements to be met by storerooms and receptacles: According to product specification
· Information about storage in one common storage facility: Not required.
· Further information about storage conditions: None.
* 8 Exposure controls and personal protection
· Additional information about design of technical systems: No further data; see item 7.
· Components with limit values that require monitoring at the workplace: Not required.
· Additional information: The lists that were valid during the creation were used as basis.
· Personal protective equipment
· General protective and hygienic measures The usual precautionary measures for handling chemicals should be followed.
· Breathing equipment: Not required.
· Protection of hands: Protective gloves.
· Material of gloves Synthetic gloves
· Penetration time of glove material
The exact break through time has to be found out by the manufacturer of the protective gloves and has to be observed.
· Eye protection: Safety glasses
* 9 Physical and chemical properties:
· General Information
Form: liquid
Color: Colorless
Odor: Odorless
· Change in condition
Melting point/Melting range: 0°C
Boiling point/Boiling range: 100°C
· Flash point: Not applicable
· Danger of explosion: Product does not present an explosion hazard.
· Vapor pressure at 20°C: 23 hPa
· Density at 20°C: 1 g/cm³
· Solubility in / Miscibility with
Water: Not miscible or difficult to mix
(Contd. on page 3)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: Bio-Rad Amplification Reagent
(Contd. of page 2)
DR
· Organic solvents: 0.0 %
· Water: 100.0 %
10 Stability and reactivity
· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
· Dangerous reactions No dangerous reactions known
· Dangerous products of decomposition: No dangerous decomposition products known
* 11 Toxicological information
· Acute toxicity:
· Primary irritant effect:
· on the skin: No irritant effect.
· on the eye: No irritant effect.
· Sensitization: No sensitizing effects known.
· Additional toxicological information:
When used and handled according to specifications, the product does not have any harmful effects according to our experience and the
information provided to us.
The substance is not subject to classification according to the latest version of the EU lists.
· Target organs: Not applicable.
* 12 Ecological information:
· General notes: Generally not hazardous for water.
13 Disposal considerations
· Product:
· Recommendation Hand over to hazardous waste disposers.
· Uncleaned packagings:
· Recommendation: Disposal must be made according to official regulations.
* 14 Transport information
· DOT regulations:
· Hazard class: -
· Land transport ADR/RID (cross-border)
· ADR/RID class: -
· Maritime transport IMDG:
· IMDG Class: -
· Marine pollutant: No
· Air transport ICAO-TI and IATA-DGR:
· ICAO/IATA Class: -
(Contd. on page 4)
USA
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Material Safety Data Sheet
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Trade name: Bio-Rad Amplification Reagent
(Contd. of page 3)
DR
· Transport/Additional information: Not dangerous according to the above specifications.
* 15 Regulations
· SARA (Superfund Amendents and Reauthorization Act of 1986 - USA)
· Section 302/304 (40CFR355.30 / 40CFR355.40):
Substance not listed.
· Section 313 (40CFR372.65):
Substance is not listed.
· TSCA (Toxic Substances Control Act):
Substance is listed.
· Cancerogenity categories
· EPA (Environmental Protection Agency)
Substance is not listed.
· IARC (International Agency for Research on Cancer)
Substance is not listed.
· NTP (National Toxicology Program)
Substance is not listed.
· TLV (Threshold Limit Value established by ACGIH)
Substance is not listed.
· MAK (German Maximum Workplace Concentration)
Substance is not listed.
· NIOSH-Ca (National Institute for Occupational Safety and Health)
Substance is not listed.
· OSHA-Ca (Occupational Safety & Health Administration)
Substance is not listed.
· Markings according to EU guidelines:
Observe the general safety regulations when handling chemicals
The substance is not subject to classification according to EU lists and other sources of literature known to us.
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous Materials (GefStoffV).
· Safety phrases:
60 This material and its container must be disposed of as hazardous waste.
· National regulations
· Water hazard class: Generally not hazardous for water.
16 Other information:
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and
shall not establish a legally valid contractual relationship.
· Department issuing MSDS: Environmental Health and Safety.
(Contd. on page 5)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: Bio-Rad Amplification Reagent
(Contd. of page 4)
DR
· Contact:
Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000
Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000
· * Data compared to the previous version altered.
USA
Page 1/5
Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
DR
* 1 Identification of substance:
· Product details:
· Trade name: 2X Amplification Diluent
· Catalog or product number: 9703205
· Application of the substance / the preparation Laboratory chemicals
· Manufacturer/Supplier:
Bio-Rad Laboratories, Life Science Group
2000 Alfred Nobel Drive
Hercules, California 94547 (510)741-1000
· Information department: Technical services, customer support.
· Emergency information:
1(800)424-9300 Use only in the event of a CHEMICAL EMERGENCY involving a SPILL, LEAK, FIRE, EXPLOSION or ACCIDENT.
510-741-1000
* 2 Composition/Information on components:
· Chemical characterization:
· CAS No. Description:
7732-18-5 water
· Identification number(s):
· EINECS Number: 231-791-2
· Additional information: For the wording of the listed risk phrases refer to section 16.
· Chemical characterization
· Listing of dangerous and non-hazardous components:
CAS: 16940-66-2
EINECS: 241-004-4
RTECS: ED3325000
Sodium borohydride T, C, F; R 15-25-34 0.1-1.0%
boric acid Xn, Xi; R 21/22-36/37/38 0.1-1.0%
* 3 Hazards identification
· Emergency overview: not applicable
· Information pertaining to particular dangers for man and environment not applicable
· Classification system
The classification was made according to OSHA 29CFR 1910.1200 and 1910.1030, and is expanded upon from company and/or
literature information.
4 First aid measures
· General information No special measures required.
· After inhalation Supply fresh air; consult doctor in case of complaints.
· After skin contact Generally the product does not irritate the skin.
· After eye contact Rinse opened eye for several minutes under running water.
· After swallowing Induce vomiting and call for medical help.
USA
(Contd. on page 2)
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: 2X Amplification Diluent
(Contd. of page 1)
DR
5 Fire fighting measures
· Suitable extinguishing agents CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
· Protective equipment: No special measures required.
6 Accidental release measures
· Person-related safety precautions: Not required
· Measures for environmental protection: Do not allow to enter sewers/ surface or ground water.
· Measures for cleaning/collecting: Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
· Additional information: No dangerous substances are released.
* 7 Handling and storage
· Handling
· Information for safe handling:
No special measures required.
No special precautions are necessary if used correctly.
· Information about protection against explosions and fires: No special measures required.
· Storage
· Requirements to be met by storerooms and receptacles: According to product specification
· Information about storage in one common storage facility: Not required.
· Further information about storage conditions: None.
* 8 Exposure controls and personal protection
· Additional information about design of technical systems: No further data; see item 7.
· Components with limit values that require monitoring at the workplace:
boric acid
TLV () Short-term value: 6 I mg/m³
Long-term value: 2 I mg/m³
· Additional information: The lists that were valid during the creation were used as basis.
· Personal protective equipment
· General protective and hygienic measures The usual precautionary measures for handling chemicals should be followed.
· Breathing equipment: Not required.
· Protection of hands: Protective gloves.
· Material of gloves Synthetic gloves
· Penetration time of glove material
The exact break through time has to be found out by the manufacturer of the protective gloves and has to be observed.
· Eye protection: Safety glasses
9 Physical and chemical properties:
· General Information
Form: liquid
Color: Colorless
(Contd. on page 3)
USA
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Material Safety Data Sheet
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Trade name: 2X Amplification Diluent
(Contd. of page 2)
DR
Odor: Odorless
· Change in condition
Melting point/Melting range: 0°C
Boiling point/Boiling range: 100°C
· Flash point: Not applicable
· Danger of explosion: Product does not present an explosion hazard.
· Vapor pressure at 20°C: 23 hPa
· Density at 20°C: 1 g/cm³
· Solubility in / Miscibility with
Water: Not miscible or difficult to mix
· Organic solvents: 0.0 %
· Water: 98.3 %
· Solids content: 1.7 %
10 Stability and reactivity
· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
· Dangerous reactions No dangerous reactions known
· Dangerous products of decomposition: No dangerous decomposition products known
* 11 Toxicological information
· Acute toxicity:
· Primary irritant effect:
· on the skin: No irritant effect.
· on the eye: No irritant effect.
· Sensitization: No sensitizing effects known.
· Additional toxicological information:
When used and handled according to specifications, the product does not have any harmful effects according to our experience and the
information provided to us.
The substance is not subject to classification according to the latest version of the EU lists.
· Target organs: Not applicable.
* 12 Ecological information:
· General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.
13 Disposal considerations
· Product:
· Recommendation Hand over to hazardous waste disposers.
(Contd. on page 4)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: 2X Amplification Diluent
(Contd. of page 3)
DR
· Uncleaned packagings:
· Recommendation: Disposal must be made according to official regulations.
14 Transport information
· DOT regulations:
· Hazard class: -
· Land transport ADR/RID (cross-border)
· ADR/RID class: -
· Maritime transport IMDG:
· IMDG Class: -
· Marine pollutant: No
· Air transport ICAO-TI and IATA-DGR:
· ICAO/IATA Class: -
· Transport/Additional information: Not dangerous according to the above specifications.
* 15 Regulations
· SARA (Superfund Amendents and Reauthorization Act of 1986 - USA)
· Section 302/304 (40CFR355.30 / 40CFR355.40):
Substance not listed.
· Section 313 (40CFR372.65):
Substance is not listed.
· TSCA (Toxic Substances Control Act):
7732-18-5 water
16940-66-2 Sodium borohydride
boric acid
7647-14-5 sodium chloride
1313-60-6 disodium peroxide
· Cancerogenity categories
· EPA (Environmental Protection Agency)
Substance is not listed.
· IARC (International Agency for Research on Cancer)
Substance is not listed.
· NTP (National Toxicology Program)
Substance is not listed.
· TLV (Threshold Limit Value established by ACGIH)
Substance is not listed.
· MAK (German Maximum Workplace Concentration)
Substance is not listed.
· NIOSH-Ca (National Institute for Occupational Safety and Health)
Substance is not listed.
(Contd. on page 5)
USA
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Material Safety Data Sheet
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Trade name: 2X Amplification Diluent
(Contd. of page 4)
DR
· OSHA-Ca (Occupational Safety & Health Administration)
Substance is not listed.
· Markings according to EU guidelines:
Observe the general safety regulations when handling chemicals
The substance is not subject to classification according to EU lists and other sources of literature known to us.
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous Materials (GefStoffV).
· Safety phrases:
60 This material and its container must be disposed of as hazardous waste.
· National regulations
· Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.
16 Other information:
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and
shall not establish a legally valid contractual relationship.
· Department issuing MSDS: Environmental Health and Safety.
· Contact:
Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000
Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000
· * Data compared to the previous version altered.
USA
Page 1/6
Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
DR
* 1 Identification of substance:
· Product details:
· Trade name: Streptavidin-HRP
· Catalog or product number: 9703206
· Application of the substance / the preparation Laboratory chemicals
· Manufacturer/Supplier:
Bio-Rad Laboratories, Life Science Group
2000 Alfred Nobel Drive
Hercules, California 94547 (510)741-1000
· Information department: Technical services, customer support.
· Emergency information:
1(800)424-9300 Use only in the event of a CHEMICAL EMERGENCY involving a SPILL, LEAK, FIRE, EXPLOSION or ACCIDENT.
510-741-1000
* 2 Composition/Information on components:
· Chemical characterization
· Description: Mixture of the substances listed below with non-hazardous additions.
· Listing of dangerous and non-hazardous components:
CAS: 7732-18-5
EINECS: 231-791-2
RTECS: ZC 0110000
water 50-100%
CAS: 99-20-7
EINECS: 202-739-6
trehalose 10-20%
CAS: 18996-35-5
EINECS: 242-734-6
sodium dihydrogen citrate 2.5-5%
CAS: 7647-14-5
EINECS: 231-598-3
sodium chloride Xi; R 36/38 1.0-2.5%
CAS: 94349-60-7
EINECS: 305-179-1
Albumins, bovine serum 0.1-1.0%
CAS: 79-07-2
EINECS: 201-174-2
RTECS: AB 5075000
2-Chloroacetamide Repr. Cat. 3; T, Xi; R 25-43-62 0.01-0.1%
· Additional information For the wording of the listed risk phrases refer to section 15.
* 3 Hazards identification
· Emergency overview:
Irritant to skin.
Sensitizer by dermal contact.
Irritant to respiratory system and skin.
Xi Irritant
· Routes of exposure: Skin
· Information pertaining to particular dangers for man and environment
The product has to be labelled due to the calculation procedure of the "General Classification guideline for preparations of the EU" in
the latest valid version.
R 43 May cause sensitization by skin contact.
(Contd. on page 2)
USA
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Material Safety Data Sheet
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Trade name: Streptavidin-HRP
(Contd. of page 1)
DR
· Classification system
The classification was made according to the latest editions of the EU-lists, and expanded upon from company and literature data.
* 4 First aid measures
· After inhalation
Supply fresh air and to be sure call for a doctor.
In case of unconsciousness place patient stably in side position for transportation.
· After skin contact Immediately wash with water and soap and rinse thoroughly.
· After eye contact Rinse opened eye for several minutes under running water. Then consult a doctor.
· After swallowing Induce vomiting and call for medical help.
5 Fire fighting measures
· Suitable extinguishing agents CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
· Protective equipment: No special measures required.
* 6 Accidental release measures
· Person-related safety precautions: Not required
· Measures for environmental protection: No special measures required.
· Measures for cleaning/collecting: Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
* 7 Handling and storage
· Handling
· Information for safe handling:
No special measures required.
No special precautions are necessary if used correctly.
· Information about protection against explosions and fires: No special measures required.
· Storage
· Requirements to be met by storerooms and receptacles: According to product specification
· Information about storage in one common storage facility: Not required.
· Further information about storage conditions: None.
* 8 Exposure controls and personal protection
· Additional information about design of technical systems: No further data; see item 7.
· Components with limit values that require monitoring at the workplace:
7647-01-0 hydrochloric acid
PEL ()
REL ()
TLV ()
Short-term value: C 7 mg/m³, C 5 ppm
Short-term value: C 7 mg/m³, C 5 ppm
Short-term value: C 2.98 mg/m³, C 2 ppm
· Additional information: The lists that were valid during the creation were used as basis.
· Personal protective equipment
· General protective and hygienic measures
Immediately remove all soiled and contaminated clothing
(Contd. on page 3)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: Streptavidin-HRP
(Contd. of page 2)
DR
Wash hands before breaks and at the end of work.
· Breathing equipment: Not required.
· Protection of hands: Protective gloves.
· Material of gloves Synthetic gloves
· Penetration time of glove material
The exact break through time has to be found out by the manufacturer of the protective gloves and has to be observed.
· Eye protection: Safety glasses
* 9 Physical and chemical properties:
· General Information
Form: liquid
Color: Colorless
Odor: Odorless
· Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: 100°C
· Flash point: Not applicable
· Auto igniting: Product is not selfigniting.
· Danger of explosion: Product does not present an explosion hazard.
· Vapor pressure at 20°C: 23.0 hPa
· Density: Not determined
· Solubility in / Miscibility with
Water: Not miscible or difficult to mix
· Solvent content:
· Organic solvents: 0.0 %
· Water: 73.8 %
· Solids content: 26.2 %
10 Stability and reactivity
· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
· Dangerous reactions No dangerous reactions known
· Dangerous products of decomposition: No dangerous decomposition products known
* 11 Toxicological information
· Acute toxicity:
· LD/LC50 values for hazardous components per OSHA criteria:
79-07-2 2-Chloroacetamide
Oral LD50 138 mg/kg (rat)
· Primary irritant effect:
· on the skin: No irritant effect.
(Contd. on page 4)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: Streptavidin-HRP
(Contd. of page 3)
DR
· on the eye: No irritant effect.
· Sensitization: Sensitization possible through skin contact.
· Additional toxicological information:
Irritant
The product shows the following dangers according to the calculation method of the General EU Classification Guidelines for
Preparations as issued in the latest version:
· Target organs: Skin.
* 12 Ecological information:
· General notes: Generally not hazardous for water.
* 13 Disposal considerations
· Product:
· Recommendation
Hand over to hazardous waste disposers.
Must not be disposed of together with household garbage. Do not allow product to reach sewage system.
· Uncleaned packagings:
· Recommendation: Disposal must be made according to official regulations.
14 Transport information
· DOT regulations:
· Hazard class: -
· Land transport ADR/RID (cross-border)
· ADR/RID class: -
· Maritime transport IMDG:
· IMDG Class: -
· Marine pollutant: No
· Air transport ICAO-TI and IATA-DGR:
· ICAO/IATA Class: -
* 15 Regulations
· SARA (Superfund Amendents and Reauthorization Act of 1986 - USA)
· Section 302/304 (40CFR355.30 / 40CFR355.40):
None of the ingredients is listed.
· Section 313 (40CFR372.65):
None of the ingredients is listed.
· TSCA (Toxic Substances Control Act):
7732-18-5 water
99-20-7 trehalose
18996-35-5 sodium dihydrogen citrate
(Contd. on page 5)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: Streptavidin-HRP
(Contd. of page 4)
DR
7647-14-5 sodium chloride
79-07-2 2-Chloroacetamide
7647-01-0 hydrochloric acid
· Cancerogenity categories
· EPA (Environmental Protection Agency)
None of the ingredients is listed.
· IARC (International Agency for Research on Cancer)
7647-01-0 hydrochloric acid 3
· NTP (National Toxicology Program)
None of the ingredients is listed.
· TLV (Threshold Limit Value established by ACGIH)
None of the ingredients is listed.
· MAK (German Maximum Workplace Concentration)
None of the ingredients is listed.
· NIOSH-Ca (National Institute for Occupational Safety and Health)
None of the ingredients is listed.
· OSHA-Ca (Occupational Safety & Health Administration)
None of the ingredients is listed.
· Markings according to EU guidelines:
Observe the general safety regulations when handling chemicals
The product has been classified and marked in accordance with EU Directives / Ordinance on Hazardous Materials
· Code letter and hazard designation of product:
Xi Irritant
· Hazard-determining components of labelling:
2-Chloroacetamide
· Risk phrases:
43 May cause sensitization by skin contact.
· Safety phrases:
24 Avoid contact with skin.
26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
37 Wear suitable gloves.
60 This material and its container must be disposed of as hazardous waste.
· National regulations
Class Share in %
I 0.01-0.1
· Water hazard class: Generally not hazardous for water.
16 Other information:
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and
shall not establish a legally valid contractual relationship.
· Department issuing MSDS: Environmental Health and Safety.
(Contd. on page 6)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: Streptavidin-HRP
(Contd. of page 5)
DR
· Contact:
Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000
Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000
· * Data compared to the previous version altered.
USA
Page 1/4
Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
DR
* 1 Identification of substance:
· Product details:
· Trade name: Blocking Reagent
· Catalog or product number: 9703207
· Application of the substance / the preparation Laboratory chemicals
· Manufacturer/Supplier:
Bio-Rad Laboratories, Life Science Group
2000 Alfred Nobel Drive
Hercules, California 94547 (510)741-1000
· Information department: Technical services, customer support.
· Emergency information:
1(800)424-9300 Use only in the event of a CHEMICAL EMERGENCY involving a SPILL, LEAK, FIRE, EXPLOSION or ACCIDENT.
510-741-1000
* 2 Composition/Information on components:
· Chemical characterization:
· CAS No. Description:
9000-71-9 Casein from bovine milk
· Identification number(s):
· EINECS Number: 232-555-1
· Additional information: For the wording of the listed risk phrases refer to section 16.
* 3 Hazards identification
· Emergency overview: not applicable
· Information pertaining to particular dangers for man and environment not applicable
· Classification system
The classification was made according to OSHA 29CFR 1910.1200 and 1910.1030, and is expanded upon from company and/or
literature information.
4 First aid measures
· General information No special measures required.
· After inhalation Supply fresh air; consult doctor in case of complaints.
· After skin contact Generally the product does not irritate the skin.
· After eye contact Rinse opened eye for several minutes under running water.
· After swallowing Induce vomiting and call for medical help.
5 Fire fighting measures
· Suitable extinguishing agents CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
· Protective equipment: No special measures required.
6 Accidental release measures
· Person-related safety precautions: Not required
· Measures for environmental protection: No special measures required.
(Contd. on page 2)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: Blocking Reagent
(Contd. of page 1)
DR
· Measures for cleaning/collecting: Pick up mechanically.
· Additional information: No dangerous substances are released.
* 7 Handling and storage
· Handling
· Information for safe handling:
No special measures required.
No special precautions are necessary if used correctly.
· Information about protection against explosions and fires: No special measures required.
· Storage
· Requirements to be met by storerooms and receptacles: According to product specification
· Information about storage in one common storage facility: Not required.
· Further information about storage conditions: None.
* 8 Exposure controls and personal protection
· Additional information about design of technical systems: No further data; see item 7.
· Components with limit values that require monitoring at the workplace: Not required.
· Additional information: The lists that were valid during the creation were used as basis.
· Personal protective equipment
· General protective and hygienic measures The usual precautionary measures for handling chemicals should be followed.
· Protection of hands: Protective gloves.
· Material of gloves Synthetic gloves
· Penetration time of glove material
The exact break through time has to be found out by the manufacturer of the protective gloves and has to be observed.
· Eye protection: Safety glasses
* 9 Physical and chemical properties:
· General Information
Form: Powder
Color: Whitish
Odor: sulphurous
· Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: undetermined
· Flash point: Not applicable
· Flammability (solid, gaseous) Product is not flammable.
· Danger of explosion: Product does not present an explosion hazard.
· Density: Not determined
· Solubility in / Miscibility with
Water: Fully miscible
· Organic solvents: 0.0 %
(Contd. on page 3)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: Blocking Reagent
(Contd. of page 2)
DR
· Solids content: 100.0 %
10 Stability and reactivity
· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
· Dangerous reactions No dangerous reactions known
· Dangerous products of decomposition: No dangerous decomposition products known
* 11 Toxicological information
· Acute toxicity:
· Primary irritant effect:
· on the skin: No irritant effect.
· on the eye: No irritant effect.
· Sensitization: No sensitizing effects known.
· Additional toxicological information:
When used and handled according to specifications, the product does not have any harmful effects according to our experience and the
information provided to us.
The substance is not subject to classification according to the latest version of the EU lists.
· Target organs: Not applicable.
* 12 Ecological information:
· General notes: Generally not hazardous for water.
13 Disposal considerations
· Product:
· Recommendation Hand over to hazardous waste disposers.
· Uncleaned packagings:
· Recommendation: Disposal must be made according to official regulations.
· Recommended cleansing agent: Water, if necessary with cleansing agents.
* 14 Transport information
· DOT regulations:
· Hazard class: -
· Land transport ADR/RID (cross-border)
· ADR/RID class: -
· Maritime transport IMDG:
· IMDG Class: -
· Marine pollutant: No
· Air transport ICAO-TI and IATA-DGR:
· ICAO/IATA Class: -
(Contd. on page 4)
USA
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Material Safety Data Sheet
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Trade name: Blocking Reagent
(Contd. of page 3)
DR
· Transport/Additional information: Not dangerous according to the above specifications.
* 15 Regulations
· SARA (Superfund Amendents and Reauthorization Act of 1986 - USA)
· Section 302/304 (40CFR355.30 / 40CFR355.40):
Substance not listed.
· Section 313 (40CFR372.65):
Substance is not listed.
· TSCA (Toxic Substances Control Act):
Substance is listed.
· Cancerogenity categories
· EPA (Environmental Protection Agency)
Substance is not listed.
· IARC (International Agency for Research on Cancer)
Substance is not listed.
· NTP (National Toxicology Program)
Substance is not listed.
· TLV (Threshold Limit Value established by ACGIH)
Substance is not listed.
· MAK (German Maximum Workplace Concentration)
Substance is not listed.
· NIOSH-Ca (National Institute for Occupational Safety and Health)
Substance is not listed.
· OSHA-Ca (Occupational Safety & Health Administration)
Substance is not listed.
· Markings according to EU guidelines:
Observe the general safety regulations when handling chemicals
The substance is not subject to classification according to EU lists and other sources of literature known to us.
The product is not subject to identification regulations under EU Directives and the Ordinance on Hazardous Materials (GefStoffV).
· National regulations
· Water hazard class: Generally not hazardous for water.
16 Other information:
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and
shall not establish a legally valid contractual relationship.
· Department issuing MSDS: Environmental Health and Safety.
· Contact:
Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000
Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000
· * Data compared to the previous version altered.
USA
Page 1/6
Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
DR
* 1 Identification of substance:
· Product details:
· Trade name: Phosphate Buffered Saline
· Catalog or product number: 9703261
· Application of the substance / the preparation Laboratory chemicals
· Manufacturer/Supplier:
Bio-Rad Laboratories, Life Science Group
2000 Alfred Nobel Drive
Hercules, California 94547 (510)741-1000
· Information department: Technical services, customer support.
· Emergency information:
1(800)424-9300 Use only in the event of a CHEMICAL EMERGENCY involving a SPILL, LEAK, FIRE, EXPLOSION or ACCIDENT.
510-741-1000
* 2 Composition/Information on components:
· Chemical characterization
· Description: Mixture of the substances listed below with non-hazardous additions.
· Listing of dangerous and non-hazardous components:
CAS: 7647-14-5
EINECS: 231-598-3
sodium chloride Xi; R 36/38 50-100%
CAS: 7558-79-4
EINECS: 231-448-7
RTECS: WC 4500000
disodium hydrogenorthophosphate Xn; R 20/21 20-35%
CAS: 7558-80-7
EINECS: 231-449-2
RTECS: WA 1900000
Sodium dihydrogen phosphate Xn; R 20/21/22 10-20%
· Additional information For the wording of the listed risk phrases refer to section 15.
* 3 Hazards identification
· Emergency overview:
Irritant to eyes.
Irritant to skin.
Irritant to respiratory system and skin.
Xn Harmful
· Routes of exposure:
Inhalation
Skin
· Information pertaining to particular dangers for man and environment
The product has to be labelled due to the calculation procedure of the "General Classification guideline for preparations of the EU" in
the latest valid version.
R 20/21 Harmful by inhalation and in contact with skin.
R 36/38 Irritating to eyes and skin.
· Classification system
The classification was made according to the latest editions of the EU-lists, and expanded upon from company and literature data.
USA
(Contd. on page 2)
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Material Safety Data Sheet
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Trade name: Phosphate Buffered Saline
(Contd. of page 1)
DR
* 4 First aid measures
· General information
Symptoms of poisoning may even occur after several hours; therefore medical observation for at least 48 hours after the accident.
· After inhalation
Supply fresh air. If required, provide artificial respiration. Keep patient warm. Consult doctor if symptoms persist.
In case of unconsciousness place patient stably in side position for transportation.
· After skin contact Immediately wash with water and soap and rinse thoroughly.
· After eye contact Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor.
· After swallowing Induce vomiting and call for medical help.
· Information for doctor
· The following symptoms may occur: Breathing difficulty
5 Fire fighting measures
· Suitable extinguishing agents CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
· Protective equipment: Mount respiratory protective device.
6 Accidental release measures
· Person-related safety precautions: Not required
· Measures for environmental protection: Do not allow to enter sewers/ surface or ground water.
· Measures for cleaning/collecting:
Dispose contaminated material as waste according to item 13.
Ensure adequate ventilation.
* 7 Handling and storage
· Handling
· Information for safe handling:
Thorough dedusting.
Ensure good ventilation/exhaustion at the workplace.
Prevent formation of dust.
No special precautions are necessary if used correctly.
· Information about protection against explosions and fires: No special measures required.
· Storage
· Requirements to be met by storerooms and receptacles: According to product specification
· Information about storage in one common storage facility: Not required.
· Further information about storage conditions: Keep receptacle tightly sealed.
* 8 Exposure controls and personal protection
· Additional information about design of technical systems: No further data; see item 7.
· Components with limit values that require monitoring at the workplace:
The product does not contain any relevant quantities of materials with critical values that have to be monitored at the workplace.
· Additional information: The lists that were valid during the creation were used as basis.
· Personal protective equipment
· General protective and hygienic measures
Keep away from foodstuffs, beverages and feed.
(Contd. on page 3)
USA
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Material Safety Data Sheet
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Trade name: Phosphate Buffered Saline
(Contd. of page 2)
DR
Immediately remove all soiled and contaminated clothing
Wash hands before breaks and at the end of work.
Avoid contact with the eyes and skin.
· Breathing equipment:
In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure use respiratory protective
device that is independent of circulating air.
· Protection of hands: Protective gloves.
· Material of gloves Synthetic gloves
· Penetration time of glove material
The exact break through time has to be found out by the manufacturer of the protective gloves and has to be observed.
· Eye protection:
Safety glasses
Tightly sealed goggles.
9 Physical and chemical properties:
· General Information
Form: Solid.
Color: White
Odor: Odorless
· Change in condition
Melting point/Melting range: undetermined
Boiling point/Boiling range: > 999°C
· Flash point: Not applicable
· Auto igniting: Product is not selfigniting.
· Danger of explosion: Product does not present an explosion hazard.
· Density: Not determined
· Solubility in / Miscibility with
Water: Fully miscible
· Solvent content:
· Organic solvents: 0.0 %
· Solids content: 100.0 %
10 Stability and reactivity
· Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications.
· Dangerous reactions No dangerous reactions known
· Dangerous products of decomposition: No dangerous decomposition products known
USA
(Contd. on page 4)
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Material Safety Data Sheet
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Trade name: Phosphate Buffered Saline
(Contd. of page 3)
DR
* 11 Toxicological information
· Acute toxicity:
· LD/LC50 values for hazardous components per OSHA criteria:
7558-80-7 Sodium dihydrogen phosphate
Oral LD50 8290 mg/kg (rat)
· Primary irritant effect:
· on the skin: Irritant to skin and mucous membranes.
· on the eye: Irritant effect.
· Sensitization: No sensitizing effects known.
· Additional toxicological information:
Harmful
Irritant
The product shows the following dangers according to the calculation method of the General EU Classification Guidelines for
Preparations as issued in the latest version:
· Target organs:
Skin.
Eye.
Respiratory tract.
* 12 Ecological information:
· General notes:
Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water.
Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system.
13 Disposal considerations
· Product:
· Recommendation
Hand over to hazardous waste disposers.
Must not be disposed of together with household garbage. Do not allow product to reach sewage system.
· Uncleaned packagings:
· Recommendation: Disposal must be made according to official regulations.
· Recommended cleansing agent: Water, if necessary with cleansing agents.
14 Transport information
· Maritime transport IMDG:
· Marine pollutant: No
* 15 Regulations
· SARA (Superfund Amendents and Reauthorization Act of 1986 - USA)
· Section 302/304 (40CFR355.30 / 40CFR355.40):
None of the ingredients is listed.
(Contd. on page 5)
USA
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Material Safety Data Sheet
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Trade name: Phosphate Buffered Saline
(Contd. of page 4)
DR
· Section 313 (40CFR372.65):
None of the ingredients is listed.
· TSCA (Toxic Substances Control Act):
All ingredients are listed.
· Cancerogenity categories
· EPA (Environmental Protection Agency)
None of the ingredients is listed.
· IARC (International Agency for Research on Cancer)
None of the ingredients is listed.
· NTP (National Toxicology Program)
None of the ingredients is listed.
· TLV (Threshold Limit Value established by ACGIH)
None of the ingredients is listed.
· MAK (German Maximum Workplace Concentration)
None of the ingredients is listed.
· NIOSH-Ca (National Institute for Occupational Safety and Health)
None of the ingredients is listed.
· OSHA-Ca (Occupational Safety & Health Administration)
None of the ingredients is listed.
· Markings according to EU guidelines:
The product has been classified and marked in accordance with EU Directives / Ordinance on Hazardous Materials
· Code letter and hazard designation of product:
Xn Harmful
· Risk phrases:
20/21 Harmful by inhalation and in contact with skin.
36/38 Irritating to eyes and skin.
· Safety phrases:
9 Keep container in a well-ventilated place.
22 Do not breathe dust.
25 Avoid contact with eyes.
26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
36/37 Wear suitable protective clothing and gloves.
60 This material and its container must be disposed of as hazardous waste.
· National regulations
· Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.
16 Other information:
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and
shall not establish a legally valid contractual relationship.
· Department issuing MSDS: Environmental Health and Safety.
(Contd. on page 6)
USA
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Material Safety Data Sheet
Printing date 01/17/2008 Reviewed on 04/25/2007
Trade name: Phosphate Buffered Saline
(Contd. of page 5)
DR
· Contact:
Life Science Group, Environmental Health and Safety, 2000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 741-1000
Diagnostic Group, Environmental Health and Safety, 4000 Alfred Nobel Drive, Hercules, California, 94547: 1(510) 724-7000
· * Data compared to the previous version altered.
USA