Eng Material Safety Data Sheet Conforms to 93/112/EC and ISO 11014-1 Responsible Name Pierce Administration Section 1. Chemical Product and Company Identification 1.800.874.3723 R-Phrases Symbol R36 R34 R20/22 Xi C Xn Product name RestoreTM Western Blot Stripping Buffer Product No. 0021059 0021062 Supplier In USA: Manufacturer Pierce Biotechnology P.O. Box 117 Rockford, IL 61105 USA 815.968.0747 or 9320 Erembodegem-Aalst Belgium Tel:+32 53 83 44 04 Fax:+32 53 83 76 38 In Europe: Pierce Perbio Science P.O. Box 117 Industriezone III Rockford, IL 61105 Industrielaan 27 USA 815.968.0747 or 1.800.874.3723 In Case of Emergency CALL CHEMTREC: Print Date 11/14/2002 Validation Date 11/4/2002 MSDS# 3432 800.424.9300 OUTSIDE US: 202.483.7616 Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. EC Number -- -- -- Section 2. Composition, Information on Ingredients Substance/Preparation Preparation : CAS No. Ingredient Name % 1) Component 1 2) Component 2 3) Component 3 5-7 0.1-1 1-3 Section 3. Hazards Identification United States Review the most current and approved institutional guideline, protocol, standard operating procedure(s) and MSDS(s) for the proper handling of institutional materials/equipment associated with the use of this product. Emergency Overview WARNING! CAUSES RESPIRATORY TRACT, EYE AND SKIN BURNS. CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: LUNGS, RESPIRATORY TRACT, SKIN, EYES, EYE, LENS OR CORNEA. MAY BE HARMFUL IF INHALED. Do not get in eyes, on skin or on clothing. Do not breathe vapor or mist. Keep container closed. Use only with adequate ventilation. Wash thoroughly after handling. Eye contact. Ingestion. Routes of Entry Potential Acute Health Effects Carcinogenic Effects Data Slightly hazardous in case of skin contact (corrosive, permeator), of eye contact (corrosive), of ingestion, of inhalation (lung corrosive). CARCINOGENIC EFFECTS: Classified 3 (Not classifiable for human.) by IARC [Component 2]. MUTAGENIC EFFECTS: Not available. TERATOGENIC EFFECTS: Not available. Repeated or prolonged exposure is not known to aggravate medical condition. Medical Conditions Aggravated by Overexposure: Overexposure /Signs/Symptoms Not available. Europe Classification R20, R34 Physical/chemical hazards Human health hazards Not applicable. Harmful by inhalation. Causes burns. Environmental hazards Not applicable. Continued on Next Page Page: 2/6 RestoreTM Western Blot Stripping Buffer See Toxicological Information (section 11) Section 4. First Aid Measures Notice to Reader Get immediate medical attention. Effects and symptoms Inhalation Slightly hazardous in case of inhalation (lung corrosive). Ingestion Slightly hazardous in case of ingestion. Skin Contact Irritation of the product in case of skin contact: Not available. Sensitization of the product: Not available. Slightly hazardous in case of skin contact (corrosive, permeator). Eye Contact Slightly hazardous in case of eye contact (corrosive). Aggravating conditions Repeated or prolonged exposure is not known to aggravate medical condition. First-Aid Measures Inhalation If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention immediately. Ingestion Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention if symptoms appear. Skin Contact In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Cold water may be used.Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention immediately. Eye Contact Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Cold water may be used. Get medical attention immediately. Notes to Physician Not available. Protection of first-aiders Not available. Section 5. Fire Fighting Measures Flammability of the Product Non-flammable. Flash Points Not applicable. Fire Hazards in Presence of Various Not applicable. Substances Fire Fighting Media Not applicable. and Instructions Protective Clothing (Fire) Not applicable. Hazardous thermal (de)composition Not applicable. products Section 6. Accidental Release Measures Personal precautions Splash goggles. Lab coat. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Gloves. Environmental Precautions and Absorb with an inert material and put the spilled material in an appropriate waste disposal. Neutralize the residue with Clean-up Methods a dilute solution of sodium carbonate. Finish cleaning by spreading water on the contaminated surface and allow to evacuate through the sanitary system. Be careful that the product is not present at a concentration level above TLV. Check TLV on the MSDS and with local authorities. Small Spill and Leak Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container. If necessary: Neutralize the residue with a dilute solution of sodium carbonate. Finish cleaning by spreading water on the contaminated surface and dispose of according to local and regional authority requirements. Continued on Next Page RestoreTM Western Blot Stripping Buffer Section 7. Handling and Storage Handling Do not breathe vapor or mist. Keep container closed. Use only with adequate ventilation. Wash thoroughly after handling. Storage Keep container tightly closed. Keep container in a cool, well-ventilated area. Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. Packaging materials Suitable / Not suitable Use original container. United States 1) Component 1 2) Component 3 Sweden 1) Component 1 2) Component 2 3) Component 3 Denmark 1) Component 1 2) Component 2 3) Component 3 Norway 1) Component 1 2) Component 2 3) Component 3 France 1) Component 1 2) Component 2 3) Component 3 Netherlands 1) Component 1 2) Component 2 3) Component 3 Section 8. Exposure Controls/Personal Protection Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective threshold limit value. Ensure that eyewash stations and safety showers are proximal to the work-station Exposure Limit Values Ingredient Name location. Occupational Exposure Limits Not available. Not available. Not available. STEL: 5 (ppm) from AFS [Sweden] [1996] Inhalation Not available. Not available. CEIL: 5 (ppm) from Arbejdstilsynet [Denmark] [1996] Inhalation Not available. Not available. CEIL: 5 (ppm) from Arbeidstilsynet [Norway] [1996] Inhalation Not available. Not available. STEL: 5 (ppm) from INRS [France] [1999] Inhalation Not available. Not available. Not available. Not available. Not available. TWA: 8 CEIL: 8 (mg/m3) from BAuA [Germany] [1999] Inhalation Not available. Germany 1) Component 1 2) Component 2 3) Component 3 Personal Protection Eyes Splash goggles. Body Lab coat. Hands Gloves. Respiratory Respirator is not needed under normal and intended conditions of use, if exposures are kept below established limits. Protective Clothing (Pictograms) Continued on Next Page Page: 3/6 Page: 4/6 RestoreTM Western Blot Stripping Buffer Not available. Odor Taste Not available. Color Clear (Light.) Section 9. Physical and Chemical Properties Liquid. Physical State and Appearance Not applicable. Molecular Weight 2.8 [Acidic.] pH (1% Soln/Water) Boiling/Condensation Point The lowest known value is 100°C (212°F) (Milli-Q Water). Melting/Freezing Point May start to solidify at -0.1°C (31.8°F) based on data for: Milli-Q Water. Specific Gravity 1 (Water = 1) Vapor Pressure The highest known value is 2.3 kPa (@ 20°C) (Milli-Q Water). Vapor Density The highest known value is 0.62 (Air = 1) (Milli-Q Water). Evaporation Rate 0.36 (Milli-Q Water) compared to Butyl acetate. Dispersion Properties See solubility in water, methanol. Solubility Easily soluble in cold water. Soluble in hot water, methanol. Insoluble in diethyl ether. Section 10. Stability and Reactivity Stability and Reactivity The product is stable. Conditions to avoid Not available. Materials to avoid Reactive with alkalis. Slightly reactive to reactive with oxidizing agents, organic materials, metals. Hazardous Polymerization Will not occur. Hazardous Decomposition Not applicable. Products Section 11. Toxicological Information Toxicity to Animals Acute oral toxicity (LD50): 3500 mg/kg [Rat]. (Component 3). Acute dermal toxicity (LD50): >3000 mg/kg [Rabbit]. (Component 3). Chronic Effects on Humans CARCINOGENIC EFFECTS: Classified 3 (Not classifiable for human.) by IARC [Component 2]. Other Toxic Effects on Humans Hazardous in case of eye contact (corrosive), of inhalation (lung corrosive). Slightly hazardous in case of ingestion, . Special Remarks on Toxicity to Not available. Animals Special Remarks on Chronic Not available. Effects on Humans Special Remarks on Other Toxic To the best of our knowledge, the toxicological properties of this product have not been thoroughly investigated. Effects on Humans (Component 3) Section 12. Ecological Information Mobility Not available. Persistence/degradability Not available. Bioaccumulative potential Not available. Ecotoxicity Not available. Continued on Next Page Page: 5/6 RestoreTM Western Blot Stripping Buffer Germany water class VCI WGK: No products were found. Section 13. Disposal Considerations Waste Information Waste must be disposed of in accordance with federal, state and local environmental control regulations. Waste Stream Not available. Consult your local or regional authorities. Section 14. Transport Information Contact the supplier for all information regarding the proper transportation method for this material. 1 Section 15. Regulatory Information Label Requirements (Europe) R20- Harmful by inhalation. R34- Causes burns. S1/2- Keep locked up and out of the reach of children. S26- In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37- Wear suitable protective clothing and gloves. S39- Wear eye/face protection. S45- In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). S46- If swallowed, seek medical advice immediately and show this container or label. Corrosive HCS Classification Irritating substance. Target organ effects. U.S. Federal Regulations TSCA 8(b) inventory: Component 1; Component 2; Milli-Q Water TSCA 8(d) H and S data reporting: Component 2 SARA 302/304/311/312 extremely hazardous substances: Component 2 SARA 302/304 emergency planning and notification: Component 2 SARA 302/304/311/312 hazardous chemicals: Component 2 SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Component 2: immediate health hazard, delayed health hazard Clean Water Act (CWA) 307: No products were found. Clean Water Act (CWA) 311: Component 2 Clean air act (CAA) 112 accidental release prevention: Component 2 Clean air act (CAA) 112 regulated flammable substances: No products were found. Clean air act (CAA) 112 regulated toxic substances: Component 2 WHMIS (Canada) CLASS D-2A: Material causing other toxic effects (VERY TOXIC). CEPA DSL: Component 1; Component 2; Milli-Q Water International Regulations Not available. EINECS DSCL (EEC) R20- Harmful by inhalation. R34- Causes burns. Continued on Next Page RestoreTM Western Blot Stripping Buffer International Lists Australia (NICNAS): Component 1; Component 2; Milli-Q Water VCI WGK: No products were found. Korea (TCCL): Component 1; Component 2; Milli-Q Water Philippines (RA6969): Component 1; Component 2; Milli-Q Water State Regulations Pennsylvania RTK: Component 2: (environmental hazard, generic environmental hazard) Massachusetts RTK: Component 2 New Jersey: Component 2 California prop. 65: No products were found. * Section 16. Other Information Health Hazardous Material Information System Fire Hazard (U.S.A.) Reactivity Personal Protection References Not available. Full text of R-Phrases referenced under headings 2 and 3: Validated by Pierce Administration on 11/4/2002. Notice to Reader History of Document Changes Any information changes since last document version are marked with a triangle symbol. Irritating to eyes. Causes burns. Harmful by inhalation and if swallowed. Intended Use Refer to the instruction booklet for proper and intended use. Otherwise, contact supplier for specific applications. To the best of our knowledge, the information contained herein is accurate. However, neither the above named supplier nor any of its subsidiaries assumes any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist. 1 0 1 h National Fire Protection Association (U.S.A.) 1 Health Verified by Pierce Administration. Printed 11/14/2002. Page: 6/6 Fire Hazard 0 Reactivity 1 Specific Hazard Date of Previous Issue No Previous Validation Version 0.01