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MSDS Safety Information 
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NAME: HEMATOXYLIN 2

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SAFETY DATA SHEET
1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND OF THE
COMPANY/UNDERTAKING
VENTANA MEDICAL SYSTEMS, INC.
1910 E. Innovation Park Drive
Tucson, Arizona 85755
U.S.A.
+1 (520) 887-2155
(800) 227-2155 (U.S.)
EC REP
Roche Diagnostics GmbH Roche Diagnostics Australia Pty. Ltd
Sandhofer Strasse 116 31 Victoria Avenue
D-68305 Mannheim Castle Hill, NSW, 2154
Germany Australia
Phone: 61-2-9860-2329
Fax: 61-2-9860-2133
Emergency Phone Number: 61-2-9759-4301
SDS NUMBER: 00232133
SUBSTANCE: Hematoxylin II
PRODUCT NUMBER(S): 790-2208
PRODUCT USE: clinical/research
CREATION DATE: Sep 06 2006
REVISION DATE: Mar 05 2009
2. HAZARDS IDENTIFICATION
NFPA RATINGS (SCALE 0-4): HEALTH=2 FIRE=1 REACTIVITY=0
EC CLASSIFICATION:
Xi Irritant
RISK PHRASES:
R 36/38 Irritating to eyes and skin.
EMERGENCY OVERVIEW:
COLOUR: red to purple
PHYSICAL FORM: liquid
ODOUR: vinegar odour
MAJOR HEALTH HAZARDS: harmful if inhaled, respiratory tract irritation, skin irritation, eye irritation,
central nervous system depression, nerve damage, kidney damage
POTENTIAL HEALTH EFFECTS:
INHALATION:
SHORT TERM EXPOSURE: irritation, cough, difficulty breathing, headache, loss of voice, drowsiness,
lung congestion, dizziness, changes in blood pressure, loss of coordination
LONG TERM EXPOSURE: tooth decay, digestive disorders, constipation, lung damage
SKIN CONTACT:
SHORT TERM EXPOSURE: irritation, skin discolouration, absorption may occur, headache, dizziness,
drowsiness, loss of coordination, heart damage, kidney damage, liver damage, nerve damage
LONG TERM EXPOSURE: irritation, skin discolouration
EYE CONTACT:
SHORT TERM EXPOSURE: irritation, lachrymation, sensitivity to light, eye damage
LONG TERM EXPOSURE: irritation, lachrymation, sensitivity to light, eye damage
INGESTION:
SHORT TERM EXPOSURE: irritation, diarrhoea, vomiting, headache, thirst, dizziness, drowsiness,
stomach pain, loss of coordination, changes in body temperature, difficulty breathing, loss of voice, lung
congestion, irregular heartbeat, blood disorders, kidney damage, convulsions, heart damage, liver damage,
nerve damage
LONG TERM EXPOSURE: irritation, diarrhoea, vomiting, stomach pain, changes in body temperature,
difficulty breathing, loss of voice, lung congestion, blood disorders, irregular heartbeat, kidney damage,
convulsions
3. COMPOSITION, INFORMATION ON INGREDIENTS
COMPONENT: Mayer's haematoxylin
CAS NUMBER: Not assigned.
EC NUMBER: Not assigned.
PERCENTAGE: 36-60
EC CLASSIFICATION: Xi
RISK PHRASES: 36/38
COMPONENT: Polyethylene glycols
CAS NUMBER: 25322-68-3
EC NUMBER: Not assigned.
PERCENTAGE: 10-30
EC CLASSIFICATION: No classification assigned.
COMPONENT: Acetic acid
CAS NUMBER: 64-19-7
EC NUMBER (EINECS): 200-580-7
PERCENTAGE: 10-30
EC CLASSIFICATION: Flammable; C
RISK PHRASES: 10-35
4. FIRST AID MEASURES
INHALATION: If adverse effects occur, remove to uncontaminated area. Give artificial respiration if not
breathing. Get immediate medical attention.
SKIN CONTACT: Wash contaminated areas with soap and water. Thoroughly clean and dry contaminated
clothing before reuse. Get medical attention, if needed.
EYE CONTACT: Flush eyes with plenty of water for at least 15 minutes. Then get immediate medical
attention.
INGESTION: If a large amount is swallowed, get medical attention.
5. FIRE FIGHTING MEASURES
FIRE AND EXPLOSION HAZARDS: Slight fire hazard.
EXTINGUISHING MEDIA: carbon dioxide, regular dry chemical, regular foam, water
FIRE FIGHTING: Move container from fire area if it can be done without risk. Avoid inhalation of
material or combustion by-products. Stay upwind and keep out of low areas.
SENSITIVITY TO MECHANICAL IMPACT: Not sensitive
SENSITIVITY TO STATIC DISCHARGE: Not sensitive
FLASH POINT: not flammable
HAZARDOUS COMBUSTION PRODUCTS:
Thermal decomposition or combustion products: oxides of carbon
6. ACCIDENTAL RELEASE MEASURES
OCCUPATIONAL RELEASE:
Stop leak if possible without personal risk. Small spills: Absorb with sand or other non-combustible material.
Collect spilled material in appropriate container for disposal.
7. HANDLING AND STORAGE
STORAGE: Store and handle in accordance with all current regulations and standards. Store between 2 C
and 8 C. See original container for storage recommendations. Keep separated from incompatible substances.
8. EXPOSURE CONTROLS/PERSONAL PROTECTION
EXPOSURE LIMITS:
Acetic acid:
10 ppm (25 mg/m3) OSHA TWA
10 ppm ACGIH TWA
15 ppm ACGIH STEL
10 ppm (25 mg/m3) NIOSH recommended TWA 10 hour(s)
15 ppm (37 mg/m3) NIOSH recommended STEL
25 mg/m3 (10 ml/m3) DFG MAK (peak limitation category - I, with excursion factor of 2)
Ethane-1,2-diol:
50 ppm (125 mg/m3) OSHA ceiling (vacated by 58 FR 35338, June 30, 1993)
100 mg/m3 ACGIH ceiling (aerosol)
26 mg/m3 (10 ml/m3) DFG MAK (peak limitation category - I, with excursion factor of 2) (cutaneous
absorption danger)
52 mg/m3 (20 ppm) EC OEL TWA (skin) (IOELV)
104 mg/m3 (40 ppm) EC OEL STEL (skin) (IOELV)
10 mg/m3 UK WEL TWA (particulate) (skin)
52 mg/m3 (20 ppm) UK WEL TWA (vapour) (skin)
104 mg/m3 (40 ppm) UK WEL STEL (vapour) (skin)
Polyethylene glycols:
Polyethylene glycols (MW >200):
10 mg/m3 AIHA WEEL TWA (aerosol)
Polyethylene glycols (MW 200-600):
1000 mg/m3 DFG MAK (peak limitation category - II, with excursion factor of 8) (inhalable fraction)
VENTILATION: Provide adequate ventilation. Ensure compliance with applicable exposure limits.
EYE PROTECTION: Safety glasses or goggles are recommended when there is a potential for eye contact.
Provide an emergency eye wash fountain and quick drench shower in the immediate work area.
CLOTHING: Lab coat or apron.
GLOVES: Wear appropriate chemical resistant gloves.
PROTECTIVE MATERIAL TYPES: vinyl, latex
RESPIRATOR: No respirator is required under normal conditions of use.
9. PHYSICAL AND CHEMICAL PROPERTIES
PHYSICAL STATE: liquid
APPEARANCE: light
COLOUR: red to purple
ODOUR: vinegar odour
BOILING POINT: Not available
FREEZING POINT: Not available
VAPOUR PRESSURE: Not available
VAPOUR DENSITY: Not available
SPECIFIC GRAVITY: Not available
WATER SOLUBILITY: miscible
PH: acidic
VOLATILITY: Not available
ODOUR THRESHOLD: Not available
EVAPORATION RATE: Not available
COEFFICIENT OF WATER/OIL DISTRIBUTION: Not available
10. STABILITY AND REACTIVITY
REACTIVITY: Stable at normal temperatures and pressure.
CONDITIONS TO AVOID: Avoid heat, flames, sparks and other sources of ignition. Avoid contact with
incompatible materials.
INCOMPATIBILITIES: oxidising materials, carbonates, phosphates, hydroxides, oxides, metals,
peroxides, permanganates, amines, alcohols
HAZARDOUS DECOMPOSITION:
Thermal decomposition or combustion products: oxides of carbon
POLYMERISATION: Will not polymerise.
11. TOXICOLOGICAL INFORMATION
Acetic acid:
IRRITATION DATA: 50 mg/24 hour(s) skin-human mild; 525 mg open skin-rabbit severe; 50 mg/24
hour(s) skin-rabbit mild; 5 mg/30 second(s) rinsed eyes-rabbit mild
TOXICITY DATA: 5620 ppm/1 hour(s) inhalation-mouse LC50; 1060 uL/kg skin-rabbit LD50; 3310
mg/kg oral-rat LD50
LOCAL EFFECTS:
Corrosive: inhalation, skin, eye, ingestion
ACUTE TOXICITY LEVEL:
Moderately toxic: inhalation, dermal absorption, ingestion
MEDICAL CONDITIONS AGGRAVATED BY EXPOSURE: eye disorders, respiratory disorders, skin
disorders and allergies
Ethane-1,2-diol:
IRRITATION DATA: 0.012 percent/3 day(s) eyes-rat; 555 mg open skin-rabbit mild; 500 mg/24 hour(s)
eyes-rabbit mild; 100 mg/1 hour(s) eyes-rabbit mild; 0.012 ppm/3 day(s) eyes-rabbit; 1440 mg/6 hour(s)
eyes-rabbit moderate
TOXICITY DATA: 9530 uL/kg skin-rabbit LD50; 4700 mg/kg oral-rat LD50
CARCINOGEN STATUS: ACGIH: A4 -Not Classifiable as a Human Carcinogen
LOCAL EFFECTS:
Irritant: inhalation, skin, eye
ACUTE TOXICITY LEVEL:
Moderately toxic: ingestion
Slightly toxic: dermal absorption
TARGET ORGANS: nervous system, kidneys
Sodium iodate:
TOXICITY DATA: 505 mg/kg oral-mouse LD50
LOCAL EFFECTS:
Irritant: inhalation, skin, eye
ACUTE TOXICITY LEVEL:
Moderately toxic: ingestion
MEDICAL CONDITIONS AGGRAVATED BY EXPOSURE: kidney disorders
Polyethylene glycols:
IRRITATION DATA: 500 mg/24 hour(s) skin-rabbit mild; 500 mg/24 hour(s) eyes-rabbit mild
MEDICAL CONDITIONS AGGRAVATED BY EXPOSURE: kidney disorders
12. ECOLOGICAL INFORMATION
Not available
13. DISPOSAL CONSIDERATIONS
Dispose in accordance with all applicable regulations.
14. TRANSPORT INFORMATION
U.S. DEPARTMENT OF TRANSPORTATION: Not regulated.
CANADIAN TRANSPORTATION OF DANGEROUS GOODS: Not regulated.
LAND TRANSPORT ADR: Not regulated.
LAND TRANSPORT RID: Not regulated.
AIR TRANSPORT IATA: Not regulated.
AIR TRANSPORT ICAO: Not regulated.
MARITIME TRANSPORT IMDG: Not regulated.
15. REGULATORY INFORMATION
U.S. REGULATIONS:
CERCLA SECTIONS 102a/103 HAZARDOUS SUBSTANCES (40 CFR 302.4):
Acetic acid: 5000 LBS RQ
Ethane-1,2-diol: 5000 LBS RQ
SARA TITLE III SECTION 302 EXTREMELY HAZARDOUS SUBSTANCES (40 CFR 355 Subpart
B): Not regulated.
SARA TITLE III SECTION 304 EXTREMELY HAZARDOUS SUBSTANCES (40 CFR 355 Subpart
C): Not regulated.
SARA TITLE III SARA SECTIONS 311/312 HAZARDOUS CATEGORIES (40 CFR 370 Subparts B
and C):
ACUTE: Yes
CHRONIC: Yes
FIRE: No
REACTIVE: No
SUDDEN RELEASE: No
SARA TITLE III SECTION 313 (40 CFR 372.65):
Ethane-1,2-diol
STATE REGULATIONS:
California Proposition 65: Not regulated.
CANADIAN REGULATIONS:
WHMIS CLASSIFICATION: D2B.
EUROPEAN REGULATIONS:
EC CLASSIFICATION (CALCULATED):
Xi Irritant
DANGER/HAZARD SYMBOL:
EC RISK AND SAFETY PHRASES:
R 36/38 Irritating to eyes and skin.
S 24/25 Avoid contact with skin and eyes.
S 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S 37 Wear suitable gloves.
S 60 This material and its container must be disposed of as hazardous waste.
16. OTHER INFORMATION
EC RISK PHRASES:
R 10 Flammable.
R 35 Causes severe burns.
R 36/38 Irritating to eyes and skin.
“RTECS®” is a United States trademark owned and licenced under authority of the U.S. Government, by
and through Symyx Software, Inc. Portions ©Copyright 2001, U.S. Government. All rights reserved.
Limitations: The information and recommendations set forth in this SDS are believed to be correct as
of this date. Ventana Medical Systems, Inc. makes no warranty with respect to the content of this SDS
and disclaims all liability from reliance thereon.