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MSDS Safety Information 
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NAME: HEMATOXYLIN, STAIN HG ADS

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Harris' Alum Hematoxylin, Mercury Free
None.
638A
Laboratory Reagent
800-424-9300 CHEMTREC (USA)
613-996-6666 CANUTEC (Canada)
24 Hours/Day: 7 Days/Week
8/19/2004
9/7/2004
Material Safety Data Sheet
Product Name
Synonym
Material Uses
Manufacturer
Product Code
Effective Date
Print Date
In Case of Emergency Call
Section 1. Product and Company Identification
For More Information Call
856-423-6300 Technical Service
Monday-Friday: 8:00 AM - 5:00 PM
Chemical Family Dye Solution
EMD Chemicals Inc.
P.O. Box 70
480 Democrat Road
Gibbstown, NJ 08027
Prior to January 1, 2003 EMD Chemicals Inc. was EM
Industries, Inc. or EM Science, Division of EM Industries,
Inc.
Section 2. Composition and Information on Ingredients
Hematoxylin 517-28-2 <1
Ethanol 64-17-5 4.76
Methanol 67-56-1 0.24
Aluminum Ammonium Sulfate, Dodecahydrate 7784-26-1 4-5
Benzoic Acid 65-85-0 <1
Sodium Iodate 7681-55-2 <1
Water 7732-18-5 >87
Component CAS # % by
Weight
Absorbed through skin. Dermal contact. Eye contact. Inhalation. Ingestion.
Emergency Overview
Section 3. Hazards Identification
Routes of Entry
Potential Acute Health Effects
May be hazardous in case of inhalation.
May be hazardous in case of eye contact (irritant).
May be hazardous in case of ingestion.
May be hazardous in case of skin contact (permeator).
Eyes
Skin
Inhalation
Ingestion
Physical State and
Appearance
Liquid.
DANGER !
COMBUSTIBLE LIQUID AND VAPOR.
VAPOR MAY CAUSE FIRE.
MAY BE HARMFUL IF INHALED, ABSORBED THROUGH SKIN OR SWALLOWED.
MAY CAUSE EYE IRRITATION.
POSSIBLE BIRTH DEFECT HAZARD.
CONTAINS MATERIAL WHICH MAY CAUSE BIRTH DEFECTS BASED ON ANIMAL DATA.
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Free
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Medical Conditions
Aggravated by
Overexposure:
Repeated or prolonged exposure is not known to aggravate medical condition.
Potential Chronic Health Effects
Carcinogenic Effects This material is not known to cause cancer in animals or humans.
Additional information See Toxicological Information (section 11)
Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything
by mouth to an unconscious person. If large quantities of this material are swallowed, call a
physician immediately. Loosen tight clothing such as a collar, tie, belt or waistband.
Check for and remove any contact lenses. In case of contact, immediately flush eyes with
plenty of water for at least 15 minutes. Cold water may be used. Get medical attention.
In case of contact, immediately flush skin with plenty of water. Remove contaminated clothing
and shoes. Cold water may be used. Wash clothing before reuse. Thoroughly clean shoes
before reuse. Get medical attention.
If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult,
give oxygen. Get medical attention.
Section 4. First Aid Measures
Eye Contact
Skin Contact
Inhalation
Ingestion
The lowest known value is 362.78°C (685°F) (ETHANOL).
Combustible.
These products are carbon oxides (CO, CO2), nitrogen oxides (NO, NO2...), sulfur oxides
(SO2, SO3...). Some metallic oxides.
Closed cup: 62.222°C (144°F).
The greatest known range is LOWER: 3.3% UPPER: 19% (ETHANOL)
SMALL FIRE: Use DRY chemical powder.
LARGE FIRE: Use water spray, fog or foam. Do not use water jet.
Flammable in presence of open flames, sparks and static discharge, of shocks, of heat.
Slightly flammable to flammable in presence of oxidizing materials.
Because of the large amount of water contained in the product, the product might be
combustible only after partial or complete evaporation.
Dangerous fire and explosion risk. Container explosion may occur under fire conditions or
when heated. Vapor may travel considerable distance to source of ignition and flash back.
(METHANOL)
Risks of explosion of the product in presence of static discharge:
Flammable in presence of open flames, sparks and static discharge.
Risks of explosion of the product in presence of mechanical impact:
Flammable in presence of shocks.
Not available.
Section 5. Fire Fighting Measures
Flammability of the
Product
Auto-ignition
Temperature
Flash Points
Flammable Limits
Products of Combustion
Fire Hazards in Presence
of Various Substances
Explosion Hazards in
Presence of Various
Substances
Fire Fighting Media
and Instructions
Special Remarks on Fire
Hazards
Special Remarks on
Explosion Hazards
Protective Clothing (Fire) Be sure to use an approved/certified respirator or equivalent.
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Free
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Section 6. Accidental Release Measures
Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate
waste disposal container.
Keep away from heat. Keep away from sources of ignition. Stop leak if without risk. Be
careful that the product is not present at a concentration level above TLV. Check TLV on the
MSDS and with local authorities.
Small Spill and Leak
Large Spill and Leak
Spill Kit Information The following EMD Chemicals Inc. SpillSolv (TM) absorbent is recommended for this product:
SX1330 Solvent Treatment Kit
Keep container in a cool, well-ventilated area. Keep container tightly closed and sealed until
ready for use. Avoid all possible sources of ignition (spark or flame). Store between 15 to
30°C (59 to 86°F).
Keep away from heat, sparks and flame. Use only with adequate ventilation. To avoid fire or
explosion, dissipate static electricity during transfer by grounding and bonding containers and
equipment before transferring material. Use explosion-proof electrical (ventilating, lighting and
material handling) equipment.
Section 7. Handling and Storage
Handling
Storage
Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of
vapors below their respective occupational exposure limits. Ensure that eyewash stations and
safety showers are proximal to the work-station location.
Splash goggles. Full suit. Vapor respirator. Boots. Gloves. A self-contained breathing
apparatus should be used to avoid inhalation of the product. Suggested protective clothing
might not be sufficient; consult a specialist BEFORE handling this product.
Hematoxylin Not available.
Ethanol AUVA (Austria, 1995).
Spitzenbegrenzung: 3800 mg/m3 3 times per shift, 60 minute(s).
Spitzenbegrenzung: 2000 ML/M3 3 times per shift, 60 minute(s).
TWA: 1900 mg/m3 8 hour(s).
TWA: 1000 ML/M3 8 hour(s).
NOHSC (Australia, 1995).
TWA: 1880 mg/m3 8 hour(s).
TWA: 1000 ppm 8 hour(s).
Lijst Grenswaarden (Belgium, 1998).
VL: 1907 mg/m3 8 hour(s).
VL: 1000 ppm 8 hour(s).
SUVA (Switzerland, 1997).
MAK: 1900 mg/m3 8 hour(s).
Section 8. Exposure Controls/Personal Protection
Engineering Controls
Personal Protection in
Case of a Large Spill
Product Name Exposure Limits
Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Wear
appropriate respirator when ventilation is inadequate.
Splash goggles.
Lab coat.
Gloves.
Not applicable.
Personal Protection
Eyes
Body
Respiratory
Hands
Feet
Protective Clothing
(Pictograms)
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Free
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MAK: 1000 ML/M3 8 hour(s).
Ministry of Health (CL, 1992).
TWA: 1500 mg/m3 8 hour(s).
TWA: 800 ppm 8 hour(s).
MAK-Werte Liste (Germany, 1998).
Spitzenbegrenzung: 1920 mg/m3 4 times per shift, 30 minute(s).
Spitzenbegrenzung: 1000 ML/M3 4 times per shift, 30 minute(s).
TWA: 960 mg/m3 8 hour(s).
TWA: 500 ML/M3 8 hour(s).
TRGS900 (Germany, 1999).
Spitzenbegrenzung: 7600 mg/m3
Spitzenbegrenzung: 4000 ML/M3
TWA: 1900 mg/m3 8 hour(s).
TWA: 1000 ML/M3 8 hour(s).
Arbejdstilsynet (Denmark, 1996).
GV: 1900 mg/m3 8 hour(s).
GV: 1000 ppm 8 hour(s).
Tyterveyslaitos (Finland, 1998).
STEL: 2500 mg/m3 15 minute(s).
STEL: 1300 ppm 15 minute(s).
TWA: 1900 mg/m3 8 hour(s).
TWA: 1000 ppm 8 hour(s).
INRS (France, 1999).
VLE: 9500 mg/m3 15 minute(s).
VLE: 5000 ppm 15 minute(s).
VME: 1900 mg/m3 8 hour(s).
VME: 1000 ppm 8 hour(s).
EH40-OES (United Kingdom (UK), 2000).
TWA: 1920 mg/m3 8 hour(s).
TWA: 1000 ppm 8 hour(s).
NAOSH (Ireland, 1999).
OEL: 1900 mg/m3 8 hour(s).
OEL: 1000 ppm 8 hour(s).
Ministry of Labour (KR, 1997).
TWA: 1900 mg/m3 8 hour(s).
TWA: 1000 ppm 8 hour(s).
Secretary of Work and Social security (MX, 1994).
CPT: 1900 mg/m3 8 hour(s).
CPT: 1000 ppm 8 hour(s).
Nationale MAC-lijst (Netherlands, 2000).
TGG 8 uur: 1000 mg/m3 8 hour(s).
TGG 8 uur: 500 ppm 8 hour(s).
NZ OSH (NZ, 1994).
TWA: 1880 mg/m3 8 hour(s).
TWA: 1000 ppm 8 hour(s).
AFS (Sweden, 1996).
TGV: 1900 mg/m3
TGV: 1000 ppm
NGV: 1000 mg/m3 8 hour(s).
NGV: 500 ppm 8 hour(s).
ACGIH TLV (United States, 2000).
TWA: 1880 mg/m3 8 hour(s).
TWA: 1000 ppm 8 hour(s).
NIOSH REL (United States, 2000).
TWA: 1900 mg/m3 10 hour(s).
TWA: 1000 ppm 10 hour(s).
OSHA Final Rule (United States, 1989).
TWA: 1900 mg/m3 8 hour(s).
TWA: 1000 ppm 8 hour(s).
Methanol ACGIH (United States, 1994). Skin
TWA: 262 mg/m3
STEL: 328 mg/m3
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Free
638A Page: 5/8
OSHA (United States, 1989). Skin
TWA: 260 mg/m3
STEL: 325 mg/m3
ACGIH (United States, 1994). Skin
STEL: 328 mg/m3 15 minute(s).
STEL: 250 ppm 15 minute(s).
TWA: 262 mg/m3 8 hour(s).
TWA: 200 ppm 8 hour(s).
NIOSH REL (United States, 1994). Skin
STEL: 325 mg/m3 15 minute(s).
STEL: 250 ppm 15 minute(s).
TWA: 260 mg/m3 10 hour(s).
TWA: 200 ppm 10 hour(s).
OSHA Final Rule (United States, 1989). Skin
STEL: 325 mg/m3 15 minute(s).
STEL: 250 ppm 15 minute(s).
TWA: 260 mg/m3 8 hour(s).
TWA: 200 ppm 8 hour(s).
Aluminum Ammonium Sulfate,
Dodecahydrate
Not available.
Benzoic Acid Not available.
Sodium Iodate Not available.
Water Not available.
The lowest known value is 78.333°C (173°F) (ETHANOL). Weighted average: 98.79°C
(209.8°F)
Liquid.
99.9% (v/v). (METHANOL.)
Not available.
May start to solidify at -0.1°C (31.8°F) based on data for: Water. Weighted average: -0.27°C
(31.5°F)
The only known value is 0.794 (Water = 1) (ETHANOL).
The highest known value is 1.59 (Air = 1) (ETHANOL).
The highest known value is 5.9 kPa (44 mmHg) (@ 20°C) (ETHANOL).
Not available.
Soluble in water.
The lowest known value is 5 ppm (ETHANOL)
Not applicable.
Odor (Slight.)
Purple.
Boiling/Condensation
Point
Melting/Freezing Point
The highest known value is 1.7 (Ethanol) Weighted average: 0.43compared to(n-Butyl Acetate
=1)
Section 9. Physical and Chemical Properties
Physical State and
Appearance
Molecular Weight
pH
Specific Gravity
Vapor Pressure
Vapor Density
Volatility
Odor Threshold
Evaporation Rate
LogKow
Solubility
Odor
Color
Molecular Formula Not applicable.
VOC 12 (%)
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Free
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The product is stable.
Slightly reactive to reactive with oxidizing agents, acids.
Will not occur.
Not available.
FLAMMABLE LIQUID AND VAPOR. (ETHANOL)
Section 10. Stability and Reactivity
Stability and Reactivity
Conditions of Instability
Incompatibility with
Various Substances
Hazardous Decomposition
Products
Hazardous Polymerization
Avoid all possible sources of ignition (spark or flame). Avoid Heat Incompatible with acetic
anhydride, metal hydrides, calcum oxychloride. (ETHANOL)
Rem/Incompatibility
Acute oral toxicity (LD50): 3450 mg/kg [Mouse]. (ETHANOL).
Acute toxicity of the vapor (LC50): 20000 ppm 10 hour(s) [Rat]. (ETHANOL).
DEVELOPMENTAL TOXICITY: Classified Reproductive system/toxin/female, Reproductive
system/toxin/male [SUSPECTED] [ETHANOL].
Contains material which may cause damage to the following organs: blood, the reproductive
system, liver.
Section 11. Toxicological Information
Toxicity
Chronic Effects on
Humans
Acute Effects on Humans May be hazardous in case of eye contact (irritant). May be hazardous in case of skin contact
(permeator). May be hazardous in case of inhalation. May be hazardous in case of ingestion.
Synergetic Products
(Toxicologically)
Not available.
Irritancy Draize Test: Not available.
Sensitization Not available.
Carcinogenic Effects This material is not known to cause cancer in animals or humans.
Toxicity to Reproductive
System
Classified Reproductive system/toxin/female, Reproductive system/toxin/male [SUSPECTED]
[ETHANOL].
Teratogenic Effects Not available.
Mutagenic Effects Not available.
RTECS Number: Hematoxylin MH7875000
Ethanol KQ6300000
Methanol PC1400000
Aluminum Ammonium Sulfate Not available.
Benzoic Acid DG0875000
Sodium Iodate NN1400000
Water ZC0110000
Not available.
Not available.
The products of degradation are less toxic than the product itself.
Section 12. Ecological Information
Ecotoxicity
BOD5 and COD
Toxicity of the Products of
Biodegradation
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U154 D002
Incineration, fuels blending or recycle. Contact your local permitted waste disposal site (TSD)
for permissible treatment sites. ALWAYS CONTACT PERMITTED WASTE DISPOSER (TSD)
TO ASSURE COMPLIANCE WITH ALL CURRENT LOCAL, STATE AND FEDERAL
REGULATIONS.
EPA Waste Number
Treatment
Section 13. Disposal Considerations
Section 14. Transport Information
DOT Classification
TDG Classification
IMO/IMDG
Classification
ICAO/IATA
Classification
Not available.
Not available.
Not available.
Not available.
U.S. Federal Regulations
Section 15. Regulatory Information
TSCA 8(b) inventory: Hematoxylin; SDA-3A; Aluminum Ammonium Sulfate, Dodecahydrate;
Benzoic Acid ; Sodium Iodate; Water
Clean Water Act (CWA) 307: No products were found.
Clean Water Act (CWA) 311: Benzoic Acid
Clean air act (CAA) 112 accidental release prevention: No products were found.
Clean air act (CAA) 112 regulated flammable substances: No products were found.
Clean air act (CAA) 112 regulated toxic substances: No products were found.
CEPA DSL: Hematoxylin; Ethanol; Methanol; Benzoic Acid ; Sodium Iodate; Water
WHMIS (Canada) CLASS B-3: Combustible liquid with a flash point between 37.8°C (100°F) and 93.3°C (200°F).
Class D-2B: Material causing other toxic effects (TOXIC).
DSCL (EEC) This product is not classified according to the EU regulations.
International Regulations
Hematoxylin 208-237-3
Ethanol 200-578-6
Methanol 200-659-6
Aluminum Ammonium Sulfate, Dodecahydrate Not available.
Benzoic Acid 200-618-2
Sodium Iodate 231-672-5
Water 231-791-2
EINECS
SARA 302/304/311/312 extremely hazardous substances: No products were found.
SARA 302/304 emergency planning and notification: No products were found.
SARA 302/304/311/312 hazardous chemicals: SDA-3A; Benzoic Acid
SARA 311/312 MSDS distribution - chemical inventory - hazard identification: SDA-3A: Fire
Hazard, Immediate (Acute) Health Hazard, Delayed (Chronic) Health Hazard; Benzoic Acid :
Immediate (Acute) Health Hazard, Delayed (Chronic) Health Hazard
This product has been classifed in accordance with the hazard criteria of the Controlled Product
Regulations and the MSDS contains all required information.
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Harris' Alum Hematoxylin, Mercury
Free
638A Page: 8/8
Pennsylvania RTK: Ethanol: (generic environmental hazard); Methanol: (environmental hazard,
generic environmental hazard); Benzoic Acid : (environmental hazard, generic environmental
hazard)
Massachusetts RTK: Ethanol; Methanol; Benzoic Acid
New Jersey: Harris' Alum Hematoxylin, Mercury Free
California prop. 65: No products were found.
State Regulations
Australia (NICNAS): Hematoxylin; Ethanol; Methanol; Aluminum Ammonium Sulfate,
Dodecahydrate; Benzoic Acid ; Sodium Iodate; Water
Germany water class: Ethanol
Japan (MITI): Hematoxylin; Ethanol; Methanol; Benzoic Acid ; Sodium Iodate; Water
Korea (TCCL): Hematoxylin; Ethanol; Methanol; Benzoic Acid ; Sodium Iodate; Water
Philippines (RA6969): Hematoxylin; Ethanol; Methanol; Aluminum Ammonium Sulfate,
Dodecahydrate; Benzoic Acid ; Sodium Iodate; Water
International Lists
China: No products were found.
+
Section 16. Other Information
Notice to Reader
The statements contained herein are based upon technical data that EMD Chemicals Inc. believes to be reliable, are offered for
information purposes only and as a guide to the appropriate precautionary and emergency handling of the material by a properly
trained person having the necessary technical skills. Users should consider these data only as a supplement to other information
gathered by them and must make independent determinations of suitability and completeness of information from all sources to
assure proper use, storage and disposal of these materials and the safety and health of employees and customers and the
protection of the environment. EMD CHEMICALS INC. MAKES NO REPRESENTATION OR WARRANTY OF ANY KIND,
EXPRESS OR IMPLIED, INCLUDING MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE, WITH RESPECT TO
THE INFORMATION HEREIN OR THE PRODUCT TO WHICH THE INFORMATION REFERS.
0
2
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National Fire
Protection
Association
(U.S.A.)
Health
Specific Hazard
Reactivity
Fire Hazard
Changed Since Last
Revision