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MSDS Safety Information 
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NAME: 3D COLLAGEN CELL CULTURE

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PAGE 1 OF 9
MSDS/SDS Number: 00000311MSDS
Latest Revision Date: August 28, 2009
Revision: A
SECTION 1 IDENTIFICATION OF THE SUBSTANCE OR
PREPARATION AND OF THE
COMPANY/UNDERTAKING
Product Name: Sodium Hydroxide Solution.
Catalogue Number(s): 90138; Component of ECM675.
Chemical Name: Aqueous solution of Sodium Hydroxide.
Synonyms: (NaOH), also known as lye and caustic soda.
Intended Product Use: Cellular Research
Manufacturer/Distributor: Millipore Corporation
(Corporate Headquarters)
Millipore S.A.S.
(European Headquarters)
Postal Address: 290 Concord Road
Billerica MA, USA
Boite Postale 116
67124 Molsheim Cedex, France
Telephone Number: +1-978-715-1335 +33(0)3 90 46 90 00
Hours of Operation: 9:00 am to 4:00 pm ET (GMT -4) 9:00 am to 4:00 pm EU CT (GMT +1)
Email: msds@millipore.com
CHEMTREC Emergency
Telephone Number:
International +1-703-527-3887 (collect)
North America 1-800-424-9300 (toll free)
SECTION 2 HAZARDS IDENTIFICATION
GHS Hazard Class: Corrosive to Metals: Category 1
Skin Corrosion/Irritation: Category 1
Serious Eye Damage/Irritation: Category 1
Acute Toxicity: Category 3 (oral)
Warning: May be corrosive to metals (H290)
Warning: Causes sever skin burns and eye (H314)
Warning: Causes serious eye damage (H318)
Signal Word and Hazard
Statement:
Warning: Toxic if swallowed (oral) (H301)
00000311MSDS REV. A Sodium Hydroxide Solution PAGE 2 OF 9
C (R34)
EU Hazard Symbol
Pictogram:
Xn (R22)
Xi (R41)
SECTION 3 COMPOSITION/INFORMATION ON INGREDIENTS
Identification of Dangerous
Components:
This product contains the substances listed below, which are defined
as dangerous substances or hazardous chemicals as defined in
European Community Directives 67/548/EEC or 1999/45/EC, and
Hazard Communication Standard 29 CFR 1910.1200.
Dangerous Component
EINECS or
ELINCS No.
CAS No.
Content
(weight percent)
EU
Hazard
Symbol
Letters*†
R
Phrases**
†
Sodium Hydroxide: 215-185-5 1310-73-2 2-3 % C R35
Identification of
Components Not Classified
as Dangerous:
This product contains the substances listed below, which are not
defined as dangerous substances or hazardous chemicals as defined
in European Community Directives 67/548/EEC or 1999/45/EC, and
Hazard Communication Standard 29 CFR 1910.1200.
Non-Dangerous Component
EINECS or
ELINCS No.
CAS No.
Content
(weight percent)
EU
Hazard
Symbol
Letters *
R
Phrases**
Water: 231-791-2 7732-18-5 98-97 % N/A N/A
* Symbol letters and categories of danger: T+ = Very toxic, T = Toxic, C = Corrosive, Xn = Harmful, Xi = Irritant,
E = Explosive, F+ = Extremely flammable, F = Very flammable, N = Dangerous for the environment, O = Oxidising.
** The full text of each R phrase is listed in Section 15.
† Symbols letters and R Phrases are assigned to each dangerous component for the highest concentration range as defined in
67/548/EEC and 1999/45/EC.
SECTION 4 FIRST AID MEASURES
Treatment Measures: Symptoms of Exposure:
Contact with Eyes: If the product contacts the eyes,
promptly wash (irrigate) the eyes
with large amounts of tepid water
for at least 15 minutes,
occasionally lifting the lower and
upper lids. Seek medical attention
immediately.
Contact with sodium hydroxide
will cause sever irritation. Very
strong solutions of sodium
hydroxide can hydrolyze proteins
in the eyes, leading to severe
burns and eye damage or, in
extreme cases, blindness.
Ingestion: Seek medical attention
immediately. Never give an
unconscious person anything by
mouth.
Ingestion of sodium hydroxide
can cause severe corrosive injury
to the lips, tongue, oral mucosa,
esophagus, and stomach.
Inhalation: If a person inhales large amounts
of the product move the exposed
person to fresh air at once. If
breathing is difficult or stops seek
immediate medical attention.
Inhalation of sodium hydroxide is
immediately irritating to the
respiratory tract. Inhalation of
sodium hydroxide dust, mist, or
aerosol may cause irritation of the
mucous membranes of the nose,
00000311MSDS REV. A Sodium Hydroxide Solution PAGE 3 OF 9
throat, and respiratory tract.
Swelling or spasms of the larynx
leading to upper-airway
obstruction and asphyxia can
occur after high dose inhalation.
Inflammation of the lungs and an
accumulation of fluid in the lungs
may also occur. Mucous
membrane irritation occurs at the
2 mg/m3. Sodium hydroxide of
sufficient strength can hydrolyze
proteins in tissues and can kill
cells in tissues.
Skin Contact: If the product contacts the skin,
immediately flush the
contaminated skin with mild soap
and water. If this chemical
penetrates clothing immediately
remove the clothing and flush the
skin with water. Seek medical
attention immediately.
Skin contact with solid sodium
hydroxide or its concentrated
solutions can cause severe burns
with deep ulcerations. Burns
appear soft and moist and are
very painful. Although contact with
concentrated solutions causes
pain and irritation within 3
minutes, contact with dilute
solutions may not cause
symptoms for several hours.
SECTION 5 FIRE FIGHTING MEASURES
Suitable Extinguishing
Media:
Use extinguishing media appropriate for the surrounding fire. This
product is compatible with commercially available extinguishing media.
Special Protective
Equipment for Firefighters:
This product does not require the use of any additional fire fighting
equipment beyond what is appropriate to the surrounding fire.
SECTION 6 ACCIDENTAL RELEASE MEASURES
Personal Precautions: Wear chemical resistant boots, clothing, eye protection, and gloves to
prevent skin contact (See Section 8).
Small Spills: Identify the spilled material(s). Barricade the spill area and notify
others in the surrounding areas. Control all sources of ignition if the
substance is flammable. Don the appropriate personal protective
equipment (See section 8). Control the movement of the spilled
product (into drains, soil, across floors etc.) with absorbent spill
materials. Collect contaminated spill material and place in container
meeting appropriate U.N. packaging requirements. Decontaminate
used equipment and affected spill area appropriately.
Large Spills: In addition to small spill precautions, determine personnel evacuation
distances. Notify appropriate authorities if necessary.
Environmental
Precautions:
Collect and dispose of contaminated materials according to
international, federal, state and local regulations. Keep away from
surface and ground water, drains, and soil.
00000311MSDS REV. A Sodium Hydroxide Solution PAGE 4 OF 9
SECTION 7 HANDLING AND STORAGE
Handling: Seek appropriate training to safely handle this product under normal
conditions. Use the recommended personal protective equipment (See
Section 8) to prevent chemical exposures. Wash hands with soap and
water before eating, drinking, or touching common items (phone,
computer, etc.) to prevent cross contamination. Use this product with
adequate ventilation. See product technical data sheet for details.
Storage: See product technical data sheet for details.
Specific use: See product technical data sheet for details.
SECTION 8 EXPOSURE CONTROL AND PERSONAL PROTECTION
Exposure Limit Values: OSHA PEL NIOSH REL ACGIH TLV Other
Sodium Hydroxide: TWA 2 mg/m3
Ceiling 2
mg/m3
Ceiling 2
mg/m3
See Below
Australia: Ceiling Concentration 2 mg/m3, JUL2008
Belgium: TWA 2 mg/m3, MAR2002
Denmark: Ceiling Concentration 2 mg/m3, OCT 2002
Finland: TWA 2 mg/m3, JAN1999
France: VME 2 mg/m3, FEB2006
Hungry: TWA 2 mg/m3, STEL 2 mg/m3, SEP2000
Japan: OEL- Continuous 2 mg/m3, APR2007
Korea: Ceiling Concentration 2 mg/m3, 2006
Mexico: Peak 2 mg/m3, 2004
The Netherlands: MAC-TGG 2 mg/m3, 2003
New Zealand: Ceiling Concentration 2 mg/m3, JAN2002
Norway: TWA 2 mg/m3, JAN1999
The Philippines: TWA 2 mg/m3, JAN1993
Poland: MAC (TWA) 0.5 mg/m3, MAC(STEL) 1 mg/m3, JAN1999
Sweden: TWA 1 mg/m3, Ceiling Concentration 2 mg/m3 (inhalable dust), JUN2005
Switzerland: MAK- week 2 mg/m3,KZG- week 2 mg/m3, DEC2006
Thailand: TWA 2 mg/m3, JAN1993
Turkey: TWA 2 mg/m3, JAN1993
United Kingdom: STEL 2 mg/m3, 2005
Normal Handling Conditions Emergency Response Conditions
Engineering Controls: General room ventilation is
adequate for the use of this
product.
Provide negative pressure
ventilation.
Respiratory Protection Use appropriate respiratory
protection.
Use appropriate respiratory
protection.
Eye Protection: Safety glasses with side shields. Chemical splash goggles or other
face protection as appropriate.
00000311MSDS REV. A Sodium Hydroxide Solution PAGE 5 OF 9
Skin Protection: Laboratory coat, adequate
chemical-resistant gloves.
Chemically resistant boots,
clothes, and impermeable gloves
as appropriate.
Environmental Exposure
Controls:
Not available. Not available.
Other Equipment: Safety shower, eyewash stations, and hand washing equipment should
be available close to the work area as needed.
SECTION 9 PHYSICAL AND CHEMICAL PROPERTIES
Appearance: Clear Colorless Liquid
Odor: None
Odor Threshold: Not Available
pH: Not Available
Melting Point/Freezing
Point:
605 °F (318 °C)
Initial Boiling Point and
Boiling Range:
(760 mm Hg): 2,534 °F (1,390 °C)
Flash Point: Not Available
Evaporation Rate, 20 ºC: Not Available
Flammability (Solid/Gas): Not Available
Explosive Limits: LEL: Not Available UEL: Not Available
Vapor Pressure: 0 mm Hg at 68 °F (20 °C)
Vapor Density, 20 ºC: Not Available
Relative Density (Water =
1.0):
2.13
Solubility: Soluble
Partition Coefficient
(n-octanol/water):
Not Available
Auto Ignition Temperature
(ASTM D1929):
Not Available
Decomposition
Temperature:
Not Available
Oxidizing Properties: None
Viscosity, Centipoise: Not Available
SECTION 10 STABILITY AND REACTIVITY
Chemical Stability: Product is stable under normal operating conditions and use as
described in the product technical data sheet.
Conditions to Avoid: See product technical data sheet for details.
00000311MSDS REV. A Sodium Hydroxide Solution PAGE 6 OF 9
Incompatible Materials to
Avoid:
Sodium hydroxide dissolves easily in water generating a great deal of
heat. It reacts with acids (also generating a lot of heat); halogenated
organic compounds; metals such as aluminum, tin, and zinc; and
nitromethane. Sodium hydroxide is corrosive to most metals.
Hazardous Decomposition
Products:
Heating to decomposition temperature may produce carbon monoxide,
carbon dioxide, nitrogen oxides.
SECTION 11 TOXICOLOGICAL INFORMATION
Toxicology Data: Toxicological information for this product as a whole does not exist,
below is data for the individual components.
Sodium Hydroxide: RTECS #WB4900000
Toxicity Test Exposure
Route
Dose Observed Effect
Acute Toxicity: Lowest
Published Lethal
Dose (Human)
Oral 1.57 mg/kg Behavioral: Anorexia
(human)
Nutritional and Gross
Metabolic: Body
temperature increase
Skin: After topical
application: Primary
irritation1
Lowest
Published Lethal
Dose (Rabbit)
Oral 500 mg/kg N/A
Lowest
Published Toxic
Concentration
(Rabbit)
Skin 25 pph Behavioral: Food intake
(animal)
Nutritional and Gross
Metabolic: Body
temperature increase
Skin: After topical
application: Primary
irritation
Skin Corrosion/Irritation: Skin Irritation
(Rabbit)
Skin 500 mg/24
hour
Severe
Contact with 25–50% solutions produces immediate irritation, while after
contact with solutions of 4% or less, irritation may not develop for several
hours. It may not be possible to correctly ascertain the degree of damage to
eyes for up to 72 hours after exposure.2
Serious Eye Damage/Eye
Irritation:
Eye Irritation
(Rabbit)
Eye 1% Severe
Respiratory or Skin
Sensitization:
Not Available
Germ Cell Mutagenicity: Not Available
Reproductive Toxicity: Not Available
STOST-Single Exposure: Not Available
STOST-Repeated
Exposure:
Not Available
Aspiration Hazard: Not Available
Carcinogenicity: Carcinogenetic information for this product as a whole does not exist,
below is data for the individual components.
00000311MSDS REV. A Sodium Hydroxide Solution PAGE 7 OF 9
Research Agency: OSHA: NTP: IARC:
Sodium Hydroxide: Not Listed Not Listed Not Listed
SECTION 12 ECOLOGICAL INFORMATION
Ecotoxicity: Ecotoxicity information for this product as a whole does not exist,
below is data for the individual components.
Sodium Hydroxide: LC50 Gambusia Affinis 24 Hours 125,000 ug/L3
LC50 Gambusia Affinis 48 Hours 125,000 ug/L3
LC50 Gambusia Affinis 96 Hours 125,000 ug/L3
Mobility: Aquatic: In the case of a solid, anhydrous sodium hydroxide spill on
soil, ground water pollution will occur if precipitation occurs prior to
clean up. Precipitation will dissolve some of the solid (with much heat
given off) and create an aqueous solution of sodium hydroxide, which
then would be able to infiltrate the soil. However, prediction of the
concentration and properties of the solution produced would be
difficult.
Persistence and
Degradation:
Not Available
Bio Accumulative
Potential:
Not Available
Results of PBT
Assessment:
Not Available
Other Adverse Effects: None known.
SECTION 13 DISPOSAL INFORMATION
Substance: Dispose of unused contents in accordance with international, federal,
state, and local regulations.
Contaminated Packaging: Dispose of container in accordance with international, federal, state
and local requirements.
SECTION 14 TRANSPORTATION INFORMATION
UN Number: UN1824
Class: 8
Proper Shipping Name: Sodium Hydroxide Solution
Packing Group: II
Marine Pollutant: Not Listed
Other Applicable
Information:
None
SECTION 15 REGULATORY INFORMATION
Australia: Hazchem Code: 2R
00000311MSDS REV. A Sodium Hydroxide Solution PAGE 8 OF 9
Poisons Schedule Number: S6
California: Proposition 65 Listed: Not Listed
Canada: WHMIS: E, D2B
European Union: REACH: Chemical Safety Assessment for
the substance or substances in
the preparation not required.
Substances of Very High Concern
(SVHC) - October 28th, 2008:
This product does not contain
SVHC’s in concentrations above
0.1% weight/weight.
Category of Danger: C: Corrosive
Xn: Harmful
Xi: Irritant
Risk Phrases: R22: Harmful if swallowed.
R24: Causes burns.
R34: Causes burns.
R35: Causes severe burns.
R41: Risk of serious damage to
eyes.
Safety Phrases: S7/9: Keep container tightly closed
and in a well-ventilated place.
S20/21: When using do not eat, drink
or smoke.
S26: In case of contact with eyes,
rinse immediately with plenty of water
and seek medical advice.
S27/28: After contact with skin, take
off immediately all contaminated
clothing, and wash immediately with
plenty of soap and tepid water.
S29/35: Do not empty into drains;
dispose of this material and its
container in a safe way.
S36/37/39: Wear suitable protective
clothing, gloves and eye/face
protection.
S45: In case of accident or if you feel
unwell, seek medical advice
immediately
OECD/High Production Volume
(HPV) Chemicals:
Sodium Hydroxide
RoHS: This product does not contain
RoHS listed substances in
concentrations above the
established thresholds.
Japan: Poisonous and Deleterious
Substances Control Law:
Sodium Hydroxide: Deleterious
Substance
SECTION 16 ADDITIONAL INFORMATION
Training Advice: Seek effective chemical handling training to reduce the hazards
associated with this product prior to use.
00000311MSDS REV. A Sodium Hydroxide Solution PAGE 9 OF 9
Technical Contact: http://www.millipore.com/support
Abbreviations Used ACGIH American Conference of Government Industrial Hygienists
ADR European agreement on the international carriage of dangerous
goods on road
CAS Chemical Abstract Service
EINECS European Inventory of Existing Commercial Chemical Substances
ELINCS European List of Notified Chemical Substances
EPA United States Environmental Protection Agency
IARC International Agency for Research in Cancer.
IATA International Air Transport Association
ICAO International Civil Aviation Organization
IMDG Regulations regarding the transportation of dangerous goods on
ocean-going vessels issued by the International Maritime
Organization.
LC50 Lethal Concentration 50% is the concentration of a chemical
which kills 50% of a sample population
LD50 Lethal Dose 50% is the dose of a chemical which kills 50% of a
sample population.
LDLo Lowest observed lethal dose
LEL Lower Explosive Limit
MSFU Manufacture, Formulation, Supply and Use (Section 13)
NIOSH National Institute of Occupational Safety and Health (US)
NTP National Toxicology Program (US)
OSHA United States Occupational Safety and Health Administration
RID International regulations concerning the international carriage of
dangerous goods by rail.
RTECS Registry of Toxic Effects of Chemical Substances (US)
STOST Specific Target Organ Systemic Toxicity
UEL Upper Explosive Limit
WHMIS Workplace Hazardous Materials Information System (Canada)
This safety data sheet has been prepared to comply with the requirements of the European Union
regulation on the Registration, Evaluation, Authorization and Restriction of Chemicals (REACH)
1906/2006 and ANSI standard Z400.1-1998.
Millipore and the M Mark are registered trademarks of Millipore Corporation.
© 2009 Millipore Corporation. All rights reserved. The above information is believed to be current and
accurate; however, Millipore makes no warranty with respect to such information and assumes no
liability for any loss or injury which may result from the use of this information. Users should conduct
their own investigations to determine the suitability of the information.
1 Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA, National Institute for
Occupational Safety and Health (NIOSH), Registry of Toxic Effects of Chemical Substances (RTECS), File
#WB4900000, 2009.
2 http://www.atsdr.cdc.gov/MHMI/mmg178.pdf, 2009.
3 Wallen, I.E., W.C. Greer, and R. Lasater, Toxicity to Gambusia affinis of Certain Pure Chemicals in Turbid
Waters, Sewage Ind.Wastes 29(6):695-711, 1957.