Material Safety Data Sheet Harris Hematoxylin ACC# 89230 Section 1 - Chemical Product and Company Identification MSDS Name: Harris Hematoxylin Catalog Numbers: BP2699-16, BP2699-1G, BP2699-4, BP2699-500 Synonyms: None Known. Company Identification: Fisher Scientific 1 Reagent Lane Fair Lawn, NJ 07410 For information, call: 201-796-7100 Emergency Number: 201-796-7100 For CHEMTREC assistance, call: 800-424-9300 For International CHEMTREC assistance, call: 703-527-3887 Section 2 - Composition, Information on Ingredients CAS# Chemical Name Percent EINECS/ELINCS 7732-18-5 Deionized Water >85 231-791-2 7784-26-1 Aluminum ammonium sulfate dodecahydrate 7 unlisted 64-17-5 Ethyl Alcohol ~5.0 200-578-6 67-56-1 Methyl Alcohol <1.0 200-659-6 7681-55-2 Sodium iodate <1 231-672-5 517-28-2 Harris Hematoxylin .5 208-237-3 Section 3 - Hazards Identification EMERGENCY OVERVIEW Appearance: maroon liquid. Flash Point: 62.2 deg C. Caution! May be absorbed through intact skin. Combustible liquid and vapor. May cause eye and skin irritation. May cause respiratory tract irritation. May cause central nervous system depression. May cause liver and kidney damage. May cause reproductive and fetal effects. Target Organs: Kidneys, central nervous system, liver. Potential Health Effects Eye: May cause severe eye irritation. May cause painful sensitization to light. Vapors may cause eye irritation. Skin: May cause skin irritation. Prolonged and/or repeated contact may cause irritation and/or dermatitis. Ingestion: May cause irritation of the digestive tract. May cause systemic toxicity with acidosis. May cause liver and kidney damage. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure. Inhalation: May cause respiratory tract irritation. May cause effects similar to those described for ingestion. May cause drowsiness, unconsciousness, and central nervous system depression. Chronic: Chronic inhalation and ingestion may cause effects similar to those of acute inhalation and ingestion. Prolonged or repeated skin contact may cause defatting and dermatitis. Section 4 - First Aid Measures Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid. Skin: Get medical aid. Rinse area with large amounts of water for at least 15 minutes. Ingestion: Call a poison control center. If swallowed, do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical aid. Inhalation: Remove from exposure and move to fresh air immediately. If breathing is difficult, give oxygen. Get medical aid. If breathing has ceased apply artificial respiration using oxygen and a suitable mechanical device such as a bag and a mask. Notes to Physician: Treat symptomatically and supportively. Section 5 - Fire Fighting Measures General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Use water spray to keep fire-exposed containers cool. Containers may explode when heated. Extinguishing Media: Use water spray to cool fire-exposed containers. Use water spray, dry chemical, carbon dioxide, or chemical foam. Cool containers with flooding quantities of water until well after fire is out. Flash Point: 62.2 deg C ( 143.96 deg F) Autoignition Temperature: Not applicable. Explosion Limits, Lower:Not available. Upper: Not available. NFPA Rating: (estimated) Health: 2; Flammability: 2; Instability: 0 Section 6 - Accidental Release Measures General Information: Use proper personal protective equipment as indicated in Section 8. Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Remove all sources of ignition. Provide ventilation. Section 7 - Handling and Storage Handling: Wash thoroughly after handling. Use only in a well-ventilated area. Avoid contact with eyes, skin, and clothing. Empty containers retain product residue, (liquid and/or vapor), and can be dangerous. Avoid ingestion and inhalation. Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or open flames. Storage: Keep away from heat, sparks, and flame. Keep away from sources of ignition. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. Section 8 - Exposure Controls, Personal Protection Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate general or local exhaust ventilation to keep airborne concentrations below the permissible exposure limits. Exposure Limits Chemical Name ACGIH NIOSH OSHA - Final PELs Deionized Water none listed none listed none listed Aluminum ammonium sulfate dodecahydrate 2 mg/m3 TWA (as Al) (listed under Aluminum, soluble salts). 2 mg/m3 TWA (as Al) (listed under Aluminum, soluble salts). none listed Ethyl Alcohol 1000 ppm TWA 1000 ppm TWA; 1900 mg/m3 TWA 3300 ppm IDLH (10% LEL) 1000 ppm TWA; 1900 mg/m3 TWA Methyl Alcohol 200 ppm TWA; 250 ppm STEL; Skin - potential significant contribution to overall exposure by the cutaneous r oute 200 ppm TWA; 260 mg/m3 TWA 6000 ppm IDLH 200 ppm TWA; 260 mg/m3 TWA Sodium iodate none listed none listed none listed Harris Hematoxylin none listed none listed none listed OSHA Vacated PELs: Deionized Water: No OSHA Vacated PELs are listed for this chemical. Aluminum ammonium sulfate dodecahydrate: No OSHA Vacated PELs are listed for this chemical. Ethyl Alcohol: 1000 ppm TWA; 1900 mg/m3 TWA Methyl Alcohol: 200 ppm TWA; 260 mg/m3 TWA Sodium iodate: No OSHA Vacated PELs are listed for this chemical. Harris Hematoxylin: No OSHA Vacated PELs are listed for this chemical. Personal Protective Equipment Eyes: Wear chemical splash goggles. Skin: Wear appropriate protective gloves to prevent skin exposure. Clothing: Wear appropriate protective clothing to prevent skin exposure. Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced. Section 9 - Physical and Chemical Properties Physical State: Liquid Appearance: maroon Odor: Slight alcohol pH: Not available. Vapor Pressure: Not available. Vapor Density: Not available. Evaporation Rate:Not available. Viscosity: Not available. Boiling Point: 100 deg C Freezing/Melting Point:Not available. Decomposition Temperature:Not available. Solubility: Soluble in water. Specific Gravity/Density:~1.05 Molecular Formula:Not available. Molecular Weight:Not available. Section 10 - Stability and Reactivity Chemical Stability: Stable under normal temperatures and pressures. Conditions to Avoid: Incompatible materials, ignition sources, excess heat. Incompatibilities with Other Materials: Acids (mineral, non-oxidizing, e.g. hydrochloric acid, hydrofluoric acid, muriatic acid, phosphoric acid), acids (mineral, oxidizing, e.g. chromic acid, hypochlorous acid, nitric acid, sulfuric acid), acids (organic, e.g. acetic acid, benzoic acid, formic acid, methanoic acid, oxalic acid), azo, diazo, and hydrazines (e.g. dimethyl hydrazine, hydrazine, methyl hydrazine), isocyanates (e.g. methyl isocyanate), metals (alkali and alkaline, e.g. cesium, potassium, sodium), nitrides (e.g. potassium nitride, sodium nitride), peroxides and hydroperoxides (organic, e.g. acetyl peroxide, benzoyl peroxide, butyl peroxide, methyl ethyl ketone peroxide), epoxides (e.g. butyl glycidyl ether), oxidizing agents (strong, e.g. bromine, hydrogen peroxide, nitrogen dioxide, potassium nitrate), reducing agents (strong, e.g. aluminum carbide, chlorosilane, hydrogen phosphide, lithium hydride), water reactive substances (e.g. acetic anyhdride, alkyl aluminum chloride, calcium carbide, ethyl dichlorosilane). Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, oxides of carbon, sodium oxide. Hazardous Polymerization: Will not occur. Section 11 - Toxicological Information RTECS#: CAS# 7732-18-5: ZC0110000 CAS# 7784-26-1: WS5640010 CAS# 64-17-5: KQ6300000 CAS# 67-56-1: PC1400000 CAS# 7681-55-2: NN1400000 CAS# 517-28-2: MH7875000 LD50/LC50: CAS# 7732-18-5: Oral, rat: LD50 = >90 mL/kg; . CAS# 7784-26-1: . CAS# 64-17-5: Draize test, rabbit, eye: 500 mg Severe; Draize test, rabbit, eye: 500 mg/24H Mild; Draize test, rabbit, skin: 20 mg/24H Moderate; Inhalation, mouse: LC50 = 39 gm/m3/4H; Inhalation, rat: LC50 = 20000 ppm/10H; Oral, mouse: LD50 = 3450 mg/kg; Oral, rabbit: LD50 = 6300 mg/kg; Oral, rat: LD50 = 7060 mg/kg; Oral, rat: LD50 = 9000 mg/kg; . CAS# 67-56-1: Draize test, rabbit, eye: 40 mg Moderate; Draize test, rabbit, eye: 100 mg/24H Moderate; Draize test, rabbit, skin: 20 mg/24H Moderate; Inhalation, rabbit: LC50 = 81000 mg/m3/14H; Inhalation, rat: LC50 = 64000 ppm/4H; Oral, mouse: LD50 = 7300 mg/kg; Oral, rabbit: LD50 = 14200 mg/kg; Oral, rat: LD50 = 5600 mg/kg; Skin, rabbit: LD50 = 15800 mg/kg; . CAS# 7681-55-2: Oral, mouse: LD50 = 505 mg/kg; . CAS# 517-28-2: . Carcinogenicity: CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65. CAS# 7784-26-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65. CAS# 64-17-5: ACGIH: Not listed. California: Not listed. NTP: Not listed. IARC: Group 1 carcinogen CAS# 67-56-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65. CAS# 7681-55-2: Not listed by ACGIH, IARC, NTP, or CA Prop 65. CAS# 517-28-2: Not listed by ACGIH, IARC, NTP, or CA Prop 65. Epidemiology: Prenatal exposure to ethanol is associated with a distinct pattern of congenital malformations that have been collectively termed the "fetal alcohol syndrome'. Among the characteristics of this syndrome are postnatal growth deficiency and physical malformations. Teratogenicity: No information found Reproductive Effects: Methanol has ben shown to produce reproductive effects in laboratory animals. Mutagenicity: Methanol has been shown to produce reproductive effects. Neurotoxicity: No information found Other Studies: Section 12 - Ecological Information No information available. Section 13 - Disposal Considerations Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification. RCRA P-Series: None listed. RCRA U-Series: CAS# 67-56-1: waste number U154 (Ignitable waste). Section 14 - Transport Information US DOT Canada TDG Shipping Name: Not regulated as a hazardous material No information available. Hazard Class: UN Number: Packing Group: Section 15 - Regulatory Information US FEDERAL TSCA CAS# 7732-18-5 is listed on the TSCA inventory. CAS# 7784-26-1 is not on the TSCA Inventory because it is a hydrate. It is considered to be listed if the CAS number for the anhydrous form is on the inventory (40CFR720.3(u)(2)). CAS# 64-17-5 is listed on the TSCA inventory. CAS# 67-56-1 is listed on the TSCA inventory. CAS# 7681-55-2 is listed on the TSCA inventory. CAS# 517-28-2 is listed on the TSCA inventory. Health & Safety Reporting List None of the chemicals are on the Health & Safety Reporting List. Chemical Test Rules None of the chemicals in this product are under a Chemical Test Rule. Section 12b None of the chemicals are listed under TSCA Section 12b. TSCA Significant New Use Rule None of the chemicals in this material have a SNUR under TSCA. CERCLA Hazardous Substances and corresponding RQs CAS# 67-56-1: 5000 lb final RQ; 2270 kg final RQ SARA Section 302 Extremely Hazardous Substances None of the chemicals in this product have a TPQ. SARA Codes CAS # 7784-26-1: immediate. CAS # 64-17-5: immediate, delayed, fire. CAS # 67-56-1: immediate, fire. CAS # 7681-55-2: immediate, fire. Section 313 This material contains Methyl Alcohol (CAS# 67-56-1, <1.0%),which is subject to the reporting requirements of Section 313 of SARA Title III and 40 CFR Part 373. Clean Air Act: CAS# 67-56-1 is listed as a hazardous air pollutant (HAP). This material does not contain any Class 1 Ozone depletors. This material does not contain any Class 2 Ozone depletors. Clean Water Act: None of the chemicals in this product are listed as Hazardous Substances under the CWA. None of the chemicals in this product are listed as Priority Pollutants under the CWA. None of the chemicals in this product are listed as Toxic Pollutants under the CWA. OSHA: None of the chemicals in this product are considered highly hazardous by OSHA. STATE CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ. CAS# 7784-26-1 can be found on the following state right to know lists: California, (listed as Aluminum, soluble salts), Pennsylvania, (listed as Aluminum, soluble salts), Minnesota, (listed as Aluminum, soluble salts). CAS# 64-17-5 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts. CAS# 67-56-1 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts. CAS# 7681-55-2 is not present on state lists from CA, PA, MN, MA, FL, or NJ. CAS# 517-28-2 is not present on state lists from CA, PA, MN, MA, FL, or NJ. California Prop 65 WARNING: This product contains Ethyl Alcohol, a chemical known to the state of California to cause developmental reproductive toxicity. California No Significant Risk Level: None of the chemicals in this product are listed. European/International Regulations European Labeling in Accordance with EC Directives Hazard Symbols: Not available. Risk Phrases: Safety Phrases: S 16 Keep away from sources of ignition - No smoking. S 33 Take precautionary measures against static discharges. S 45 In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). S 28B After contact with skin, wash immediately with plenty of water and soap. WGK (Water Danger/Protection) CAS# 7732-18-5: No information available. CAS# 7784-26-1: 1 CAS# 64-17-5: 0 CAS# 67-56-1: 1 CAS# 7681-55-2: 1 CAS# 517-28-2: 1 Canada - DSL/NDSL CAS# 7732-18-5 is listed on Canada's DSL List. CAS# 7784-26-1 is listed on Canada's DSL List. CAS# 64-17-5 is listed on Canada's DSL List. CAS# 67-56-1 is listed on Canada's DSL List. CAS# 7681-55-2 is listed on Canada's DSL List. CAS# 517-28-2 is listed on Canada's DSL List. Canada - WHMIS This product has a WHMIS classification of D2B, B3. This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations. Canadian Ingredient Disclosure List CAS# 7784-26-1 (listed as Aluminum, soluble salts) is listed on the Canadian Ingredient Disclosure List. CAS# 64-17-5 is listed on the Canadian Ingredient Disclosure List. CAS# 67-56-1 is listed on the Canadian Ingredient Disclosure List. Section 16 - Additional Information MSDS Creation Date: 3/03/1998 Revision #10 Date: 4/28/2008 The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.