Industrial applications: Analytical chemistry. Research.
Material Safety Data Sheet
Material uses
Validation date
Print date
In case of emergency
Section 1. Chemical product and company identification
Catalogue Number RPN2132 90RPN2132
Supplier GE Healthcare Bio-Sciences AB
SE-751 84 Uppsala
Sweden
+46 (0)18 612 0000
ChemTrec (US) 1-800-424-9300
1-703-527-3887 ChemTrec (US)
US
Canada
United States
English
GE Healthcare
8 September 2006
08 September 2006
Lumigen PS-3 detection reagent, Solution A; part of
'ECL Plus™ Western Blotting Detection Reagent,
for 1000 cm2'
Product name
Dermal contact. Eye contact. Inhalation.
Emergency overview
Routes of entry
Potential acute health effects
Moderately irritating to the respiratory system.
Irritating to eyes.
No known significant effects or critical hazards.
Irritating to skin.
Eyes
Skin
Inhalation
Ingestion
Physical state Liquid.
See toxicological information (section 11)
Warning!
CAUSES EYE AND SKIN IRRITATION.
MAY CAUSE RESPIRATORY TRACT IRRITATION.
Avoid contact with skin and clothing. Avoid breathing vapor or mist. Keep container closed. Use only with
adequate ventilation. Wash thoroughly after handling.
CARCINOGENIC EFFECTS: Not available.
MUTAGENIC EFFECTS: Not available.
TERATOGENIC EFFECTS: Not available.
Medical conditions aggravated by
over-exposure
Repeated skin exposure can produce local skin destruction or dermatitis. Repeated or prolonged
exposure to the substance can produce lung damage. Repeated or prolonged contact with spray or mist
may produce chronic eye irritation and severe skin irritation.
Potential chronic health effects
Hazards identification 2.
Odorless. Odor
This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). OSHA/HCS status
Article Number
25006277-1
9525006277-1 Version 3
Validation date 8 September 2006
Page: 1/5
Lumigen PS-3 detection reagent, Solution A; part of 'ECL Plus™ Western Blotting Detection Reagent,
for 1000 cm2'
RPN2132
Tris(hydroxymethyl)aminomethane 2.94
Composition/information on ingredients
Name CAS number % by weight Exposure limits
3.
Do not ingest. Get medical attention if symptoms appear.
In case of contact with eyes, rinse immediately with plenty of water. Get medical attention if irritation
occurs.
Wash with soap and water. Get medical attention if symptoms appear.
If inhaled, remove to fresh air. Get medical attention if symptoms appear.
Section 4. First aid measures
Eye contact
Skin contact
Inhalation
Ingestion
No action shall be taken involving any personal risk or without suitable training. Protection of first-aiders
Non-flammable.
Section 5. Fire fighting measures
Flammability of the product
Special exposure hazards
No specific hazard.
Special protective equipment for
fire-fighters
Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive pressure mode.
Use an extinguishing agent suitable for the surrounding fire.
None known.
Extinguishing media
Suitable
Not suitable
Immediately contact emergency personnel. Keep unnecessary personnel away. Use suitable protective
equipment.
If emergency personnel are unavailable, contain spilled material. For small spills, add absorbent (soil may
be used in the absence of other suitable materials), scoop up material and place in a sealable, liquid-proof
container for disposal. For large spills, dike spilled material or otherwise contain material to ensure runoff
does not reach a waterway. Place spilled material in an appropriate container for disposal.
Environmental precautions
Section 6. Accidental release measures
Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers.
Personal precautions
Methods for cleaning up
Keep container tightly closed. Keep container in a cool, well-ventilated area.
Avoid contact with eyes, skin and clothing. Keep container closed. Use only with adequate ventilation.
Avoid breathing vapor or mist. Wash thoroughly after handling.
Section 7. Handling and storage
Handling
Storage
Section 8. Exposure controls, personal protection
Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk
assessment indicates this is necessary. Respirator selection must be based on known or anticipated
exposure levels, the hazards of the product and the safe working limits of the selected respirator.
Safety eyewear complying with an approved standard should be used when a risk assessment indicates
this is necessary to avoid exposure to liquid splashes, mists, gases or dusts.
Personal protective equipment for the body should be selected based on the task being performed and
the risks involved and should be approved by a specialist before handling this product.
Personal protection
Eyes
Skin
Respiratory
Consult local authorities for acceptable exposure limits.
Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times
when handling chemical products if a risk assessment indicates this is necessary.
Hands
Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and
using the lavatory and at the end of the working period. Appropriate techniques should be used to
remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that
eyewash stations and safety showers are close to the workstation location.
Hygiene measures
No special ventilation requirements. Good general ventilation should be sufficient to control airborne
levels. If this product contains ingredients with exposure limits, use process enclosures, local exhaust
ventilation or other engineering controls to keep worker exposure below any recommended or statutory
limits.
Engineering measures
Article Number
25006277-1
9525006277-1 Version 3
Validation date 8 September 2006
Page: 2/5
Lumigen PS-3 detection reagent, Solution A; part of 'ECL Plus™ Western Blotting Detection Reagent,
for 1000 cm2'
RPN2132
The lowest known value is 100°C (212°F) (water). Weighted average: 100.1°C (212.2°F)
Liquid.
0% (v/v)
8 (Conc. (% w/w): 100) [Basic.]
May start to solidify at 0°C (32°F) based on data for: water.
The only known value is 1.11 (Water = 1) (proprietary).
The highest known value is 3.2 kPa (23.8 mm Hg) (at 20°C) (water). Weighted average: 3.17 kPa (23.78 mm
Hg) (at 20°C)
See solubility in water, methanol, diethyl ether, acetone.
Easily soluble in cold water, hot water, methanol, diethyl ether, acetone.
The lowest known value is 374.3°C (705.7°F) (water).
Odorless.
Colorless.
Boiling/condensation point
Melting/freezing point
0.36 (water) compared with Butyl acetate.
Section 9. Physical and chemical properties
Physical state
pH
Critical temperature
Relative density
Vapor pressure
Volatility
Evaporation rate
Dispersibility properties
Solubility
Odor
Color
0 (g/l). VOC
The product is stable.
Reactive or incompatible with the following materials: oxidizing materials and reducing materials.
Slightly reactive or incompatible with the following materials: moisture.
Non-reactive or compatible with the following materials: combustible materials, organic materials, metals,
acids and alkalis.
Will not occur.
Section 10. Stability and reactivity
Stability and reactivity
Incompatibility with various
substances
Hazardous polymerization
Non-flammable in the presence of the following materials or conditions: open flames, sparks and static
discharge, heat, shocks and mechanical impacts, oxidizing materials, reducing materials, combustible
materials, organic materials, metals, acids, alkalis and moisture.
Non-explosive in the presence of the following materials or conditions: open flames, sparks and static
discharge, heat, shocks and mechanical impacts, oxidizing materials, reducing materials, combustible
materials, organic materials, metals, acids, alkalis and moisture.
Conditions of reactivity
Section 11. Toxicological information
Specific effects
Carcinogenic effects No known significant effects or critical hazards.
Mutagenic effects No known significant effects or critical hazards.
Teratogenicity / Reproduction
toxicity
No known significant effects or critical hazards.
Not considered to be toxic to humans. Other toxic effects on humans
Toxicity data
Product/ingredient name
Tris(hydroxymethyl)aminomethane LD50
LDLo
5900 mg/kg
1000 mg/kg
Oral
Oral
Rat
Rabbit
Test Result Route Species
Sensitization
Irritating to skin.
Moderately irritating to the respiratory system.
No known significant effects or critical hazards.
Irritating to eyes.
Ingestion
Inhalation
Eyes
Skin
These products are carbon oxides (CO, CO2) and water, nitrogen oxides (NO, NO2 etc.).
The product itself and its products of degradation are not toxic.
Section 12. Ecological information
Toxicity of the products of
biodegradation
Products of degradation
No known significant effects or critical hazards. Environmental precautions
Article Number
25006277-1
9525006277-1 Version 3
Validation date 8 September 2006
Page: 3/5
Lumigen PS-3 detection reagent, Solution A; part of 'ECL Plus™ Western Blotting Detection Reagent,
for 1000 cm2'
RPN2132
The generation of waste should be avoided or minimized wherever possible. Avoid dispersal of spilled
material and runoff and contact with soil, waterways, drains and sewers. Disposal of this product,
solutions and any by-products should at all times comply with the requirements of environmental
protection and waste disposal legislation and any regional local authority requirements.
Waste disposal
Section 13. Disposal considerations
Consult your local or regional authorities.
Section 14. Transport information
Not classified.
International transport regulations
Irritating material HCS Classification
Section 15. Regulatory information
U.S. Federal regulations
Pennsylvania RTK: proprietary: (environmental hazard, generic environmental hazard)
Massachusetts RTK: proprietary
New Jersey: proprietary; proprietary
TSCA 8(b) inventory: proprietary; proprietary; proprietary; proprietary; proprietary; water
Clean Water Act (CWA) 307: No products were found.
Clean Water Act (CWA) 311: proprietary
Clean Air Act (CAA) 112 accidental release prevention: No products were found.
Clean Air Act (CAA) 112 regulated flammable substances: No products were found.
Clean Air Act (CAA) 112 regulated toxic substances: No products were found.
State regulations
Australia (NICNAS): proprietary; proprietary; proprietary; proprietary; proprietary; water
China: proprietary; proprietary; proprietary; proprietary; proprietary; water
Germany water class: proprietary; proprietary; proprietary; proprietary; proprietary
Japan (METI): proprietary; proprietary; proprietary; water
Korea (TCCL): proprietary; proprietary; proprietary; proprietary; water
Philippines (RA6969): proprietary; proprietary; proprietary; proprietary; proprietary; water
This product is not classified according to EU legislation. Risk phrases
International regulations
International lists
SARA 302/304/311/312 extremely hazardous substances: No products were found.
SARA 302/304 emergency planning and notification: No products were found.
SARA 302/304/311/312 hazardous chemicals: No products were found.
SARA 311/312 MSDS distribution - chemical inventory - hazard identification: No products were found.
EU regulations
Section 16. Other information
Hazardous Material Information
System (U.S.A.)
0
0
2
National Fire Protection
Association (U.S.A.) Health
Special
Instability
Flammability
CAUSES EYE AND SKIN IRRITATION.
MAY CAUSE RESPIRATORY TRACT IRRITATION.
Label requirements
History
Health
Fire hazard
Reactivity
Personal protection
0
0
0
B
Indicates information that has changed from previously issued version.
Article Number
25006277-1
9525006277-1 Version 3
Validation date 8 September 2006
Page: 4/5
Lumigen PS-3 detection reagent, Solution A; part of 'ECL Plus™ Western Blotting Detection Reagent,
for 1000 cm2'
RPN2132
Notice to reader
To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its
subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein.
Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should
be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.
Date of printing 08 September 2006 Date of previous issue 31 October 2005
3 Version 08 September 2006 Date of issue
Article Number
25006277-1
9525006277-1 Version 3
Validation date 8 September 2006
Page: 5/5