ÿþPage 1 of 29 @Beckman CoulterTM Kit MSDS Doc. ID: 33000-75: Rev. AB Product Information Product Name VITAMIN B12 Part Number 33000 Doc. ID 33000-75 AB Issued (year/month/day) Revised (year/month/day) 2003/07/24 Series Name: ACCESSèø Components Description VITAMIN B12 (Compartment R1a) VITAMIN B12 (Compartment R1b) VITAMIN B12 (Compartment R1c) VITAMIN B12 (Compartment R1d) VITAMIN B12 (Compartment R1e) Transport Information Shipping Name Chemical kit ICAO UN/ID Number 3316 IATA Packing Group II IATA Hazard Class 9 Miscellaneous Dangerous Goods Subsidiary Risk None Special Provisions A44 IATA ERG Code 9L IMDG Hazard Class 9 Miscellaneous dangerous substances and articles Subsidiary Risk None Special Provisions 251 Marine Pollutant No US DOT Hazard Class 9 Miscellaneous hazardous material Subsidiary Risk None Special Provisions 15 NAERG Number 171 European ADR ADR Classification 9 Miscellaneous dangerous substances and articles Subsidiary Risk None Classification Code M11 éø2003 Beckman Coulter, Inc. Global MSDS  English VITAMIN B12 Doc. ID: 33000-75 AB Page 2 of 29 Transport Information (Continued) Canadian TDG PIN 3316 TDG Classification 9 Miscellaneous Dangerous Goods Subsidiary Risk None Special Provisions 65 NAERG Number 171 Page 3 of 29 @Beckman CoulterTM MATERIAL SAFETY DATA SHEET Section 1 Company and Product Identification Product Name VITAMIN B12 (Compartment R1a) Part Number Component of P/N 33000 VITAMIN B12 Doc. ID 33000-75 AB Issued (year/month/day) Revised (year/month/day) 2003/07/24 Series Name ACCESSèø Manufacturer Beckman Coulter, Inc. 4300 Harbor Blvd. Fullerton, CA 92835-3100, U.S.A. Distributor and Emergency Phone No.  Refer to attached list, Document ID: 472050, for local distributor and emergency phone numbers. Section 2 Composition and Information on Ingredients Hazardous Ingredients: Meets Hazardous Criteria: Chemical Name CAS # % by wt. EU US OSHA WHMIS Tris(hydroxymethyl)aminomethane 77-86-1 <5.0 No Yes Exempt See Section 15 Regulatory Information for additional information on hazard classifications. Section 3 Hazards Identification Emergency Overview Reddish-brown; Opaque; Liquid; Mild odor Nonflammable aqueous solution. Skin, eye and respiratory tract irritant. Contains animal material which may have come in contact with human material. Physical Hazards Sodium azide forms explosive compounds with heavy metals. This product contains concentrations of azide <0.1% (w/w) which with repeated contact with lead and copper commonly found in plumbing drains may result in the build up of shock sensitive compounds. Potential Health Effects Summary Exposure may result in irritation of skin, eyes, and mucous membranes. See Section 11 Toxicological Information for more detailed health information. Product Hazard Classifications EU: Not applicable WHMIS: Exempt US OSHA: Hazardous Beckman Coulter Safety Rating Flammability (Section V): 0 Health (Section XI): 1 Reactivity with Water (Section X): 0 Contact (Section VIII): 2 Code 0=none 1=slight 2=caution 3=severe VITAMIN B12 Doc. ID: 33000-75 AB Page 4 of 29 VITAMIN B12 (Compartment R1a) Section 4 First Aid Measures Inhalation If product is inhaled, move exposed individual to fresh air. If individual is not breathing, begin artificial respiration immediately and obtain medical attention. Eye Contact If product enters eyes, wash eyes gently under running water for 15 minutes or longer, making sure that the eyelids are held open. If pain or irritation occur, obtain medical attention. Skin Contact In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and shoes. If pain or irritation occur, obtain medical attention. Ingestion If ingested, wash mouth out with water. If irritation or discomfort occurs, seek medical attention. Section 5 Fire Fighting Measures Flash Point Not applicable Flammable Limits Not applicable Autoignition Temp. Not applicable Extinguishing Media Use extinguishing media suitable for surrounding fire. Special Fire and Explosion Hazards No special hazards determined. Hazardous Combustion Products Due to the composition and volume of this product, combustion products generated from it are not expected to present a significant hazard. Protective Equipment for Firefighters Self-contained breathing apparatus is recommended for firefighters. Section 6 Accidental Release Measures Personal Precautions This product contains a material of biological origin. Use universal precautions during clean up procedures. Spill and Leak Procedures As a precautionary measure, treat spilled material with a 1:10 bleach/water solution. Absorb liquid and place in container suitable for disposal. Avoid generation of aerosols during clean up. Comply with applicable waste disposal regulations. Environmental Precautions Contain spill to prevent migration. Section 7 Handling and Storage Handling Precautions This product should be handled as though capable of transmitting infectious diseases. Universal precautions should be followed when using this product. Recommended Storage Conditions Keep away from incompatible material. To maintain efficacy, store according to the instructions in the product labeling. Section 8 Exposure Controls and Personal Protection Exposure Limits US OSHA: None established VITAMIN B12 Doc. ID: 33000-75 AB Page 5 of 29 VITAMIN B12 (Compartment R1a) Section 8 Exposure Controls and Personal Protection (Continued) ACGIH: None established DFG MAK: None established Engineering Controls No special engineering controls are required. Use with good general ventilation. Respiratory Protection Under normal conditions, the use of this product should not require respiratory protection. If overexposure should occur and ventilation is not adequate to maintain airborne concentrations at acceptable levels, the use of respiratory protection should be evaluated by a qualified professional. Eye Protection Safety glasses or chemical goggles should be worn to prevent eye contact. Skin Protection Impervious gloves, such as latex or equivalent, should be worn to prevent skin contact. Section 9 Physical and Chemical Properties Physical State Liquid Color Reddish-brown Transparency Opaque Odor Mild odor Odor Threshold Not applicable pH 8.0 Boiling Point Not available Melting Point Not available Specific Gravity 1.01 @20°C Vapor Pressure Not available Vapor Density Not available Evaporation Rate Not available Solubility Water Miscible Organic Not available Section 10 Stability and Reactivity Stability Stable under normal temperatures and pressures. Hazardous Incompatibilities Metals and metallic compounds Strong acids Strong oxidizers Sodium azide forms explosive compounds with heavy metals. This product contains concentrations of azide <0.1% (w/w) which with repeated contact with lead and copper commonly found in plumbing drains may result in the build up of shock sensitive compounds. Hazardous Decomposition Products When stored as labeled, no known hazardous decomposition products are formed during the shelf-life of this product. VITAMIN B12 Doc. ID: 33000-75 AB Page 6 of 29 VITAMIN B12 (Compartment R1a) Section 10 Stability and Reactivity (Continued) Conditions to Avoid Keep away from incompatible material. Section 11 Toxicological Information Toxicity Data for Hazardous Ingredients Tris(hydroxymethyl)- aminomethane Oral LD50 Rat: 5900 mg/kg Primary Routes of Exposure Common routes of entry include inhalation, ingestion and eye/skin contact. Specific paths of concern for potentially infectious materials are skin puncture, contact with broken skin, contact with mucous membranes and inhalation of aerosolized material. Potential Effects of Acute Exposure Exposure to TRIS may result in irritation of skin, eyes and mucous membrane. Eye contact may result in redness, pain and corneal injury. Inhalation may result in chest pain and tightness, coughing, and difficult breathing. Ingestion may cause gastrointestinal irritation with burns to mouth and stomach, and large doses may cause weakness, collapse and death in laboratory animals. This product contains materials of animal origin, some of which may have come into contact with human material during processing. The product should be considered as potentially capable of transmitting infectious diseases. Potential Effects of Chronic Exposure Prolonged or repeated exposure to TRIS by skin contact may result in dermatitis. Eye contact may cause conjunctivitis. Symptoms of Overexposure Symptoms of overexposure may include: throat irritation and coughing; dry, red, cracked skin; red irritated eyes; headache, drowsiness, dizziness, stupor; convulsions and coma. Carcinogenicity No ingredients in this product are listed as carcinogens by ACGIH, IARC, NTP, OSHA or 67/548/EEC Annex I. Other Effects None identified. Conditions Aggravated by Exposure None identified. Section 12 Ecological Information Ecotoxicity Toxic to fish and other water organisms. Biodegradability No information available. Mobility No information available. Section 13 Disposal Considerations Waste Disposal Dispose of waste product, unused product and contaminated packaging in compliance with federal, state and local regulations. If unsure of the applicable requirements, contact the authorities for information. Sodium azide preservative may form explosive compounds in metal drain lines. See NIOSH Bulletin: Explosive Azide Hazard (8/16/76). Section 14 Transport Information Transportation of this product is not regulated under ICAO, IMDG, US DOT, European ADR or Canadian TDG. VITAMIN B12 Doc. ID: 33000-75 AB Page 7 of 29 VITAMIN B12 (Compartment R1a) Section 15 Regulatory Information US Federal and State Regulations SARA 313 Sodium Azide is subject to reporting requirements of Section 313, Title III of SARA. Zinc Chloride is subject to reporting requirements of Section 313, Title III of SARA. CERCLA RG s, 40 CFR 302.4 Zinc Chloride is listed. Sodium Azide is listed. California Proposition 65 Gentamicin Sulfate has been identified by the State of California to cause reproductive harm. The State of California has adopted a regulation which requires a warning be given to individuals who may be exposed to chemicals identified by the State to cause cancer or reproductive harm. Accordingly, Beckman Coulter advises you of the following warning: WARNING: This product contains a chemical known to the State of California to cause reproductive harm. Massachusetts MSL Zinc Chloride is listed. Sodium Azide is listed. Magnesium Chloride is listed. New Jersey Dept. of Health RTK List Zinc Chloride is listed. Sodium Azide is listed. Magnesium Chloride is listed. Pennsylvania RTK Zinc Chloride is listed. Sodium Azide is listed. Magnesium Chloride is listed. EU Labeling Classification Preparation not classified. Canada This product is exempt from WHMIS label and MSDS requirements. PIN: Not applicable Ingredients on Ingredient Disclosure List: Zinc Chloride Sodium Azide Ingredients with unknown toxicological properties: Product is exempt Section 16 Other Information For further information, please contact your local Beckman Coulter representative. WHILE BECKMAN COULTER, INC. BELIEVES THE INFORMATION CONTAINED HEREIN IS VALID AND ACCURATE, BECKMAN COULTER MAKES NO WARRANTY OR REPRESENTATION AS TO ITS VALIDITY, ACCURACY, OR CURRENCY. BECKMAN COULTER SHALL NOT BE LIABLE OR OTHERWISE RESPONSIBLE IN ANYWAY FOR USE OF EITHER THIS INFORMATION OR MATERIALS TO WHICH IT APPLIES. DISPOSAL OF HAZARDOUS MATERIALS MAY BE SUBJECT TO LOCAL LAWS OR REGULATIONS. Printed in U.S.A. Page 8 of 29 @Beckman CoulterTM MATERIAL SAFETY DATA SHEET Section 1 Company and Product Identification Product Name VITAMIN B12 (Compartment R1b) Part Number Component of P/N 33000 VITAMIN B12 Doc. ID 33000-75 AB Issued (year/month/day) Revised (year/month/day) 2003/07/24 Series Name ACCESSèø Manufacturer Beckman Coulter, Inc. 4300 Harbor Blvd. Fullerton, CA 92835-3100, U.S.A. Distributor and Emergency Phone No.  Refer to attached list, Document ID: 472050, for local distributor and emergency phone numbers. Section 2 Composition and Information on Ingredients Hazardous Ingredients: Meets Hazardous Criteria: Chemical Name CAS # % by wt. EU US OSHA WHMIS Sodium Borate Decahydrate 1303-96-4 <5.0 No Yes Exempt See Section 15 Regulatory Information for additional information on hazard classifications. Section 3 Hazards Identification Emergency Overview Yellow; Clear; Liquid; Mild odor Nonflammable aqueous solution. Skin, eye and respiratory tract irritant. Physical Hazards Sodium azide forms explosive compounds with heavy metals. This product contains concentrations of azide <0.1% (w/w) which with repeated contact with lead and copper commonly found in plumbing drains may result in the build up of shock sensitive compounds. Potential Health Effects Summary May cause irritation of respiratory tract, skin and eyes; central nervous system depression; severe kidney damage and reproductive effects. See Section 11 Toxicological Information for more detailed health information. Product Hazard Classifications EU: Not applicable WHMIS: Exempt US OSHA: Hazardous Beckman Coulter Safety Rating Flammability (Section V): 0 Health (Section XI): 1 Reactivity with Water (Section X): 0 Contact (Section VIII): 2 Code 0=none 1=slight 2=caution 3=severe VITAMIN B12 Doc. ID: 33000-75 AB Page 9 of 29 VITAMIN B12 (Compartment R1b) Section 4 First Aid Measures Inhalation If product is inhaled, move exposed individual to fresh air. If individual is not breathing, begin artificial respiration immediately and obtain medical attention. Eye Contact If product enters eyes, wash eyes gently under running water for 15 minutes or longer, making sure that the eyelids are held open. If pain or irritation occur, obtain medical attention. Skin Contact In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and shoes. If pain or irritation occur, obtain medical attention. Ingestion If ingested, wash mouth out with water. If irritation or discomfort occurs, seek medical attention. Section 5 Fire Fighting Measures Flash Point Not applicable Flammable Limits Not applicable Autoignition Temp. Not applicable Extinguishing Media Use extinguishing media suitable for surrounding fire. Special Fire and Explosion Hazards No special hazards determined. Hazardous Combustion Products Due to the composition and volume of this product, combustion products generated from it are not expected to present a significant hazard. Protective Equipment for Firefighters Self-contained breathing apparatus is recommended for firefighters. Section 6 Accidental Release Measures Personal Precautions Avoid skin contact. Use good laboratory procedures. Spill and Leak Procedures Absorb spilled material with an appropriate inert, non-flammable absorbent and dispose according to local regulations. Environmental Precautions Contain spill to prevent migration. Section 7 Handling and Storage Handling Precautions Use good laboratory procedures; avoid eye and skin contact. Recommended Storage Conditions Keep away from incompatible material. To maintain efficacy, store according to the instructions in the product labeling. Section 8 Exposure Controls and Personal Protection Exposure Limits US OSHA: None established VITAMIN B12 Doc. ID: 33000-75 AB Page 10 of 29 VITAMIN B12 (Compartment R1b) Section 8 Exposure Controls and Personal Protection (Continued) ACGIH: Sodium Borate Decahydrate 5 mg/m3 TWA DFG MAK: None established Engineering Controls No special engineering controls are required. Use with good general ventilation. Respiratory Protection Under normal conditions, the use of this product should not require respiratory protection. If overexposure should occur and ventilation is not adequate to maintain airborne concentrations at acceptable levels, the use of respiratory protection should be evaluated by a qualified professional. Eye Protection Safety glasses or chemical goggles should be worn to prevent eye contact. Skin Protection Impervious gloves, such as latex or equivalent, should be worn to prevent skin contact. Section 9 Physical and Chemical Properties Physical State Liquid Color Yellow Transparency Clear Odor Mild odor Odor Threshold Not applicable pH 6.6 Boiling Point Not available Melting Point Not available Specific Gravity 1.017 @20°C Vapor Pressure Not available Vapor Density Not available Evaporation Rate Not available Solubility Water Miscible Organic Not available Section 10 Stability and Reactivity Stability Stable under normal temperatures and pressures. Hazardous Incompatibilities Metals and metallic compounds Strong acids Strong bases Strong oxidizers Sodium azide forms explosive compounds with heavy metals. This product contains concentrations of azide <0.1% (w/w) which with repeated contact with lead and copper commonly found in plumbing drains may result in the build up of shock sensitive compounds. VITAMIN B12 Doc. ID: 33000-75 AB Page 11 of 29 VITAMIN B12 (Compartment R1b) Section 10 Stability and Reactivity (Continued) Hazardous Decomposition Products When stored as labeled, no known hazardous decomposition products are formed during the shelf-life of this product. Conditions to Avoid Keep away from incompatible material. Section 11 Toxicological Information Toxicity Data for Hazardous Ingredients Sodium Borate Decahydrate Oral LD50 Rat: 2660 mg/kg; Oral LD50 Mouse: 2 gm/kg Primary Routes of Exposure The most likely routes of exposure are skin, eye contact and inhalation; material may be absorbed through the skin. Potential Effects of Acute Exposure Ingestion or absorption of concentrated borates through the skin may cause systemic effects including nausea, vomiting, diarrhea and restlessness. Extremely high concentrations may result in central nervous system and cardiovascular disorders with delirium, convulsions, coma and kidney damage. May cause skin and eye irritation. Potential Effects of Chronic Exposure Chronic skin absorption or ingestion of borates may result in conditions similar to those described for acute exposure. Repeated or prolonged skin and eye contact may cause dermatitis and conjunctivitis with irritation and redness. Symptoms of Overexposure Symptoms may include: coughing, headache, fatigue, nausea, vomiting, dizziness, blurred vision, red and watery eyes, abdominal pain and unconsciousness. Carcinogenicity No ingredients in this product are listed as carcinogens by ACGIH, IARC, NTP, OSHA or 67/548/EEC Annex I. Other Effects Reproductive effects have been reported in test animals exposed to borates by ingestion. Conditions Aggravated by Exposure None identified. Section 12 Ecological Information Ecotoxicity Toxic to fish and other water organisms. Biodegradability No information available. Mobility No information available. Section 13 Disposal Considerations Waste Disposal Dispose of waste product, unused product and contaminated packaging in compliance with federal, state and local regulations. If unsure of the applicable requirements, contact the authorities for information. Sodium azide preservative may form explosive compounds in metal drain lines. See NIOSH Bulletin: Explosive Azide Hazard (8/16/76). Section 14 Transport Information Transportation of this product is not regulated under ICAO, IMDG, US DOT, European ADR or Canadian TDG. VITAMIN B12 Doc. ID: 33000-75 AB Page 12 of 29 VITAMIN B12 (Compartment R1b) Section 15 Regulatory Information US Federal and State Regulations SARA 313 Sodium Azide is subject to reporting requirements of Section 313, Title III of SARA. CERCLA RG s, 40 CFR 302.4 Sodium Azide is listed. California Proposition 65 No ingredients listed Massachusetts MSL Sodium Borate Decahydrate is listed. Sodium Azide is listed. New Jersey Dept. of Health RTK List Sodium Borate Decahydrate is listed. Sodium Azide is listed. Pennsylvania RTK Sodium Borate Decahydrate is listed. Sodium Azide is listed. EU Labeling Classification Preparation not classified. Canada This product is exempt from WHMIS label and MSDS requirements. PIN: Not applicable Ingredients on Ingredient Disclosure List: Sodium Borate Decahydrate Sodium Azide Ingredients with unknown toxicological properties: Product is exempt Section 16 Other Information For further information, please contact your local Beckman Coulter representative. WHILE BECKMAN COULTER, INC. BELIEVES THE INFORMATION CONTAINED HEREIN IS VALID AND ACCURATE, BECKMAN COULTER MAKES NO WARRANTY OR REPRESENTATION AS TO ITS VALIDITY, ACCURACY, OR CURRENCY. BECKMAN COULTER SHALL NOT BE LIABLE OR OTHERWISE RESPONSIBLE IN ANYWAY FOR USE OF EITHER THIS INFORMATION OR MATERIALS TO WHICH IT APPLIES. DISPOSAL OF HAZARDOUS MATERIALS MAY BE SUBJECT TO LOCAL LAWS OR REGULATIONS. Printed in U.S.A. Page 13 of 29 @Beckman CoulterTM MATERIAL SAFETY DATA SHEET Section 1 Company and Product Identification Product Name VITAMIN B12 (Compartment R1c) Part Number Component of P/N 33000 VITAMIN B12 Doc. ID 33000-75 AB Issued (year/month/day) Revised (year/month/day) 2003/07/24 Series Name ACCESSèø Manufacturer Beckman Coulter, Inc. 4300 Harbor Blvd. Fullerton, CA 92835-3100, U.S.A. Distributor and Emergency Phone No.  Refer to attached list, Document ID: 472050, for local distributor and emergency phone numbers. Section 2 Composition and Information on Ingredients Hazardous Ingredients: Meets Hazardous Criteria: Chemical Name CAS # % by wt. EU US OSHA WHMIS Tris(hydroxymethyl)aminomethane 77-86-1 <5 No Yes Exempt ProClin* 300  <0.5 C;R34 No Exempt See Section 15 Regulatory Information for additional information on hazard classifications. Section 3 Hazards Identification Emergency Overview Colorless; Translucent; Liquid; Mild odor Nonflammable aqueous solution. Skin, eye and respiratory tract irritant. Contains material of human and animal origin. Physical Hazards Sodium azide forms explosive compounds with heavy metals. This product contains concentrations of azide <0.1% (w/w) which with repeated contact with lead and copper commonly found in plumbing drains may result in the build up of shock sensitive compounds. Potential Health Effects Summary Exposure may result in irritation of skin, eyes, and mucous membranes. See Section 11 Toxicological Information for more detailed health information. Product Hazard Classifications EU: Xi;R43 WHMIS: Exempt US OSHA: Hazardous VITAMIN B12 Doc. ID: 33000-75 AB Page 14 of 29 VITAMIN B12 (Compartment R1c) Section 3 Hazards Identification (Continued) Beckman Coulter Safety Rating Flammability (Section V): 0 Health (Section XI): 1 Reactivity with Water (Section X): 0 Contact (Section VIII): 2 Code 0=none 1=slight 2=caution 3=severe Section 4 First Aid Measures Inhalation If product is inhaled, move exposed individual to fresh air. If individual is not breathing, begin artificial respiration immediately and obtain medical attention. Eye Contact If product enters eyes, wash eyes gently under running water for 15 minutes or longer, making sure that the eyelids are held open. If pain or irritation occur, obtain medical attention. Skin Contact In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and shoes. If pain or irritation occur, obtain medical attention. Ingestion If ingested, wash mouth out with water. If irritation or discomfort occurs, seek medical attention. Section 5 Fire Fighting Measures Flash Point Not applicable Flammable Limits Not applicable Autoignition Temp. Not applicable Extinguishing Media Use extinguishing media suitable for surrounding fire. Special Fire and Explosion Hazards No special hazards determined. Hazardous Combustion Products Due to the composition and volume of this product, combustion products generated from it are not expected to present a significant hazard. Protective Equipment for Firefighters Self-contained breathing apparatus is recommended for firefighters. Section 6 Accidental Release Measures Personal Precautions This product contains a material of biological origin. Use universal precautions during clean up procedures. Spill and Leak Procedures As a precautionary measure, treat spilled material with a 1:10 bleach/water solution. Absorb liquid and place in container suitable for disposal. Avoid generation of aerosols during clean up. Comply with applicable waste disposal regulations. Environmental Precautions Contain spill to prevent migration. Section 7 Handling and Storage Handling Precautions This product should be handled as though capable of transmitting infectious diseases. Universal precautions should be followed when using this product. Recommended Storage Conditions Keep away from incompatible material. To maintain efficacy, store according to the instructions in the product labeling. VITAMIN B12 Doc. ID: 33000-75 AB Page 15 of 29 VITAMIN B12 (Compartment R1c) Section 8 Exposure Controls and Personal Protection Exposure Limits US OSHA: None established ACGIH: None established DFG MAK: None established Engineering Controls No special engineering controls are required. Use with good general ventilation. Respiratory Protection Under normal conditions, the use of this product should not require respiratory protection. If overexposure should occur and ventilation is not adequate to maintain airborne concentrations at acceptable levels, the use of respiratory protection should be evaluated by a qualified professional. Eye Protection Safety glasses or chemical goggles should be worn to prevent eye contact. Skin Protection Impervious gloves, such as latex or equivalent, should be worn to prevent skin contact. Section 9 Physical and Chemical Properties Physical State Liquid Color Colorless Transparency Translucent Odor Mild odor Odor Threshold Not applicable pH 8.0 Boiling Point Not available Melting Point Not available Specific Gravity 1.088 @20°C Vapor Pressure Not available Vapor Density Not available Evaporation Rate Not available Solubility Water Miscible Organic Not available Section 10 Stability and Reactivity Stability Stable under normal temperatures and pressures. VITAMIN B12 Doc. ID: 33000-75 AB Page 16 of 29 VITAMIN B12 (Compartment R1c) Section 10 Stability and Reactivity (Continued) Hazardous Incompatibilities Metals and metallic compounds Strong oxidizers Strong acids Sodium azide forms explosive compounds with heavy metals. This product contains concentrations of azide <0.1% (w/w) which with repeated contact with lead and copper commonly found in plumbing drains may result in the build up of shock sensitive compounds. Hazardous Decomposition Products When stored as labeled, no known hazardous decomposition products are formed during the shelf-life of this product. Conditions to Avoid Keep away from incompatible material. Section 11 Toxicological Information Toxicity Data for Hazardous Ingredients Tris(hydroxymethyl)- aminomethane Oral LD50 Rat: 5900 mg/kg Primary Routes of Exposure Common routes of entry include inhalation, ingestion and eye/skin contact. Specific paths of concern for potentially infectious materials are skin puncture, contact with broken skin, contact with mucous membranes and inhalation of aerosolized material. Potential Effects of Acute Exposure Exposure to TRIS may result in irritation of skin, eyes and mucous membrane. Eye contact may result in redness, pain and corneal injury. Inhalation may result in chest pain and tightness, coughing, and difficult breathing. Ingestion may cause gastrointestinal irritation with burns to mouth and stomach, and large doses may cause weakness, collapse and death in laboratory animals. Exposure to ProClin 300 may result in irritation of skin, eyes and mucous membranes. Skin contact may result in possible sensitization. Eye contact may cause clouding of the cornea. Ingestion may cause gastrointestinal irritation; large doses given to animals resulted in collapse and coma. This product contains materials of human and animal origin and should be considered as potentially capable of transmitting infectious diseases. Potential Effects of Chronic Exposure Prolonged or repeated exposure to TRIS by skin contact may result in dermatitis. Eye contact may cause conjunctivitis. Frequent or long-term eye and skin exposure to ProClin 300 may result in conjunctivitis and dermatitis. Symptoms of Overexposure Symptoms of overexposure may include: throat irritation and coughing; dry, red, cracked skin; red irritated eyes; headache, drowsiness, dizziness, stupor; convulsions and coma. Carcinogenicity No ingredients in this product are listed as carcinogens by ACGIH, IARC, NTP, OSHA or 67/548/EEC Annex I. Other Effects None identified. Conditions Aggravated by Exposure None identified. Section 12 Ecological Information Ecotoxicity Toxic to fish and other water organisms. Biodegradability No information available. Mobility No information available. VITAMIN B12 Doc. ID: 33000-75 AB Page 17 of 29 VITAMIN B12 (Compartment R1c) Section 13 Disposal Considerations Waste Disposal Dispose of waste product, unused product and contaminated packaging in compliance with federal, state and local regulations. If unsure of the applicable requirements, contact the authorities for information. Sodium azide preservative may form explosive compounds in metal drain lines. See NIOSH Bulletin: Explosive Azide Hazard (8/16/76). Section 14 Transport Information Transportation of this product is not regulated under ICAO, IMDG, US DOT, European ADR or Canadian TDG. Section 15 Regulatory Information US Federal and State Regulations SARA 313 Sodium Azide is subject to reporting requirements of Section 313, Title III of SARA. Zinc Chloride is subject to reporting requirements of Section 313, Title III of SARA. CERCLA RG s, 40 CFR 302.4 Zinc Chloride is listed. Sodium Azide is listed. California Proposition 65 No ingredients listed Massachusetts MSL Sucrose is listed. Zinc Chloride is listed. Sodium Azide is listed. Magnesium Chloride, Hexahydrate is listed. New Jersey Dept. of Health RTK List Zinc Chloride is listed. Sodium Azide is listed. Magnesium Chloride, Hexahydrate is listed. Pennsylvania RTK Sucrose is listed. Zinc Chloride is listed. Sodium Azide is listed. Magnesium Chloride, Hexahydrate is listed. EU Labeling Classification Classification Risk and Safety Phrases Xi Irritant R43 May cause sensitization by skin contact. Canada This product is exempt from WHMIS label and MSDS requirements. PIN: Not applicable Ingredients on Ingredient Disclosure List: Zinc Chloride Sodium Azide Ingredients with unknown toxicological properties: Product is exempt VITAMIN B12 Doc. ID: 33000-75 AB Page 18 of 29 VITAMIN B12 (Compartment R1c) Section 16 Other Information * ProClin is a trademark of the Rohm and Haas Company. For further information, please contact your local Beckman Coulter representative. WHILE BECKMAN COULTER, INC. BELIEVES THE INFORMATION CONTAINED HEREIN IS VALID AND ACCURATE, BECKMAN COULTER MAKES NO WARRANTY OR REPRESENTATION AS TO ITS VALIDITY, ACCURACY, OR CURRENCY. BECKMAN COULTER SHALL NOT BE LIABLE OR OTHERWISE RESPONSIBLE IN ANYWAY FOR USE OF EITHER THIS INFORMATION OR MATERIALS TO WHICH IT APPLIES. DISPOSAL OF HAZARDOUS MATERIALS MAY BE SUBJECT TO LOCAL LAWS OR REGULATIONS. Printed in U.S.A. Page 19 of 29 @Beckman CoulterTM MATERIAL SAFETY DATA SHEET Section 1 Company and Product Identification Product Name VITAMIN B12 (Compartment R1d) Part Number Component of P/N 33000 VITAMIN B12 Doc. ID 33000-75 AB Issued (year/month/day) Revised (year/month/day) 2003/07/24 Series Name ACCESSèø Manufacturer Beckman Coulter, Inc. 4300 Harbor Blvd. Fullerton, CA 92835-3100, U.S.A. Distributor and Emergency Phone No.  Refer to attached list, Document ID: 472050, for local distributor and emergency phone numbers. Section 2 Composition and Information on Ingredients Hazardous Ingredients: Meets Hazardous Criteria: Chemical Name CAS # % by wt. EU US OSHA WHMIS Sodium Hydroxide 1310-73-2 <2.0 C;R 35 Yes Exempt See Section 15 Regulatory Information for additional information on hazard classifications. Section 3 Hazards Identification Emergency Overview Colorless; Clear; Liquid; Slight almond odor Nonflammable aqueous solution. Skin, eye and respiratory tract irritant. Physical Hazards No physical hazards were determined from a review of available literature. Potential Health Effects Summary Exposure may result in irritation of skin, eyes, and mucous membranes. See Section 11 Toxicological Information for more detailed health information. Product Hazard Classifications EU: Xi;R36/38 WHMIS: Exempt US OSHA: Hazardous Beckman Coulter Safety Rating Flammability (Section V): 0 Health (Section XI): 2 Reactivity with Water (Section X): 0 Contact (Section VIII): 2 Code 0=none 1=slight 2=caution 3=severe VITAMIN B12 Doc. ID: 33000-75 AB Page 20 of 29 VITAMIN B12 (Compartment R1d) Section 4 First Aid Measures Inhalation If product is inhaled, move exposed individual to fresh air. If individual is not breathing, begin artificial respiration immediately and obtain medical attention. Eye Contact If product enters eyes, wash eyes gently under running water for 15 minutes or longer, making sure that the eyelids are held open. If pain or irritation occur, obtain medical attention. Skin Contact In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and shoes. If pain or irritation occur, obtain medical attention. Ingestion If ingested, wash mouth out with water. If irritation or discomfort occurs, seek medical attention. Section 5 Fire Fighting Measures Flash Point Not applicable Flammable Limits Not applicable Autoignition Temp. Not applicable Extinguishing Media Use extinguishing media suitable for surrounding fire. Special Fire and Explosion Hazards No special hazards determined. Hazardous Combustion Products Due to the composition and volume of this product, combustion products generated from it are not expected to present a significant hazard. Protective Equipment for Firefighters Self-contained breathing apparatus is recommended for firefighters. Section 6 Accidental Release Measures Personal Precautions Avoid skin contact. Use good laboratory procedures. Spill and Leak Procedures Absorb spilled material with an appropriate inert, non-flammable absorbent and dispose according to local regulations. Environmental Precautions Contain spill to prevent migration. Section 7 Handling and Storage Handling Precautions Avoid inhaling, ingesting, and contact with eyes and skin. Recommended Storage Conditions Keep away from incompatible material. To maintain efficacy, store according to the instructions in the product labeling. Section 8 Exposure Controls and Personal Protection Exposure Limits US OSHA: Sodium Hydroxide 2 mg/m3 TWA VITAMIN B12 Doc. ID: 33000-75 AB Page 21 of 29 VITAMIN B12 (Compartment R1d) Section 8 Exposure Controls and Personal Protection (Continued) ACGIH: Sodium Hydroxide 2 mg/m3 Ceiling DFG MAK: None established Engineering Controls No special engineering controls are required. Use with good general ventilation. Respiratory Protection Under normal conditions, the use of this product should not require respiratory protection. If overexposure should occur and ventilation is not adequate to maintain airborne concentrations at acceptable levels, the use of respiratory protection should be evaluated by a qualified professional. Eye Protection Safety glasses or chemical goggles should be worn to prevent eye contact. Skin Protection Impervious gloves, such as latex or equivalent, should be worn to prevent skin contact. Section 9 Physical and Chemical Properties Physical State Liquid Color Colorless Transparency Clear Odor Slight almond odor Odor Threshold Not applicable pH 13.5 Boiling Point Not available Melting Point Not available Specific Gravity 1.02 @20°C Vapor Pressure Not available Vapor Density Not available Evaporation Rate Not available Solubility Water Miscible Organic Not available Section 10 Stability and Reactivity Stability Stable under normal temperatures and pressures. Hazardous Incompatibilities Strong acids Peroxides Hazardous Decomposition Products When stored as labeled, no known hazardous decomposition products are formed during the shelf-life of this product. Conditions to Avoid Keep away from incompatible material. VITAMIN B12 Doc. ID: 33000-75 AB Page 22 of 29 VITAMIN B12 (Compartment R1d) Section 11 Toxicological Information Toxicity Data for Hazardous Ingredients Not applicable Primary Routes of Exposure The most likely routes of exposure are skin, eye contact and inhalation. Potential Effects of Acute Exposure Inhalation of dilute alkaline mists may result in irritation of mucous membranes with sore throat, coughing, difficulty in breathing; intense exposure, the lungs may fill with fluid. Contact with dilute solutions may cause irritation or skin injury with redness, fissures and white patches. Eye contact may result in severe burns with possible loss of sight, irritation of eyes and eyelids, pain and intense watering. Ingestion may result in burns to the mouth, throat and stomach with burning sensations in the mouth, abdominal pain, vomiting, diarrhea, shock and loss of consciousness. Potential Effects of Chronic Exposure Repeated skin contact with dilute alkaline may cause irritation with redness and swelling or burns. Repeated eye contact may cause conjunctivitis with redness, swelling and watering or burns. Depending upon concentration, repeated ingestion may result in inflammatory or ulcerative effects on the mouth with redness, swelling and sores in the mouth. Symptoms of Overexposure Symptoms may include: coughing, headache, fatigue, nausea, vomiting, dizziness, blurred vision, red and watery eyes, abdominal pain and unconsciousness. Carcinogenicity No ingredients in this product are listed as carcinogens by ACGIH, IARC, NTP, OSHA or 67/548/EEC Annex I. Other Effects None identified. Conditions Aggravated by Exposure Individuals with eye, skin or kidney disorders may find these conditions aggravated by exposure to this product. Section 12 Ecological Information Ecotoxicity Toxic to fish and other water organisms. Biodegradability No information available. Mobility No information available. Section 13 Disposal Considerations Waste Disposal Dispose of waste product, unused product and contaminated packaging in compliance with federal, state and local regulations. If unsure of the applicable requirements, contact the authorities for information. VITAMIN B12 Doc. ID: 33000-75 AB Page 23 of 29 VITAMIN B12 (Compartment R1d) Section 14 Transport Information International Shipping Information Shipping Name Chemical kit ICAO UN/ID Number 3316 IATA Packing Group II IATA Hazard Class 9 Miscellaneous Dangerous Goods Subsidiary Risk None Special Provisions A44 IATA ERG Code 9L IMDG Hazard Class 9 Miscellaneous dangerous substances and articles Subsidiary Risk None Special Provisions 251 Marine Pollutant No US DOT Hazard Class 9 Miscellaneous hazardous material Subsidiary Risk None Special Provisions 15 NAERG Number 171 European ADR ADR Classification 9Miscellaneous dangerous substances and articles Subsidiary Risk None Classification Code M11 Canadian TDG PIN 3316 TDG Classification 9 Miscellaneous Dangerous Goods Subsidiary Risk None Special Provisions 65 NAERG Number 171 Section 15 Regulatory Information US Federal and State Regulations SARA 313 Potassium Cyanide is subject to reporting requirements of Section 313, Title III of SARA. CERCLA RG s, 40 CFR 302.4 Potassium Cyanide is listed. Sodium Hydroxide is listed. California Proposition 65 No ingredients listed Massachusetts MSL Potassium Cyanide is listed. Sodium Hydroxide is listed. New Jersey Dept. of Health RTK List Potassium Cyanide is listed. Sodium Hydroxide is listed. Pennsylvania RTK Potassium Cyanide is listed. Sodium Hydroxide is listed. VITAMIN B12 Doc. ID: 33000-75 AB Page 24 of 29 VITAMIN B12 (Compartment R1d) Section 15 Regulatory Information (Continued) EU Labeling Classification Classification Risk and Safety Phrases Xi Irritant R36/38 Irritating to eyes and skin. S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S37/39 Wear suitable gloves and eye/face protection. Canada This product is exempt from WHMIS label and MSDS requirements. PIN: 3316 Ingredients on Ingredient Disclosure List: Sodium Hydroxide Ingredients with unknown toxicological properties: Product is exempt Section 16 Other Information For further information, please contact your local Beckman Coulter representative. WHILE BECKMAN COULTER, INC. BELIEVES THE INFORMATION CONTAINED HEREIN IS VALID AND ACCURATE, BECKMAN COULTER MAKES NO WARRANTY OR REPRESENTATION AS TO ITS VALIDITY, ACCURACY, OR CURRENCY. BECKMAN COULTER SHALL NOT BE LIABLE OR OTHERWISE RESPONSIBLE IN ANYWAY FOR USE OF EITHER THIS INFORMATION OR MATERIALS TO WHICH IT APPLIES. DISPOSAL OF HAZARDOUS MATERIALS MAY BE SUBJECT TO LOCAL LAWS OR REGULATIONS. Printed in U.S.A. Page 25 of 29 @Beckman CoulterTM MATERIAL SAFETY DATA SHEET Section 1 Company and Product Identification Product Name VITAMIN B12 (Compartment R1e) Part Number Component of P/N 33000 VITAMIN B12 Doc. ID 33000-75 AB Issued (year/month/day) Revised (year/month/day) 2003/07/24 Series Name ACCESSèø Manufacturer Beckman Coulter, Inc. 4300 Harbor Blvd. Fullerton, CA 92835-3100, U.S.A. Distributor and Emergency Phone No.  Refer to attached list, Document ID: 472050, for local distributor and emergency phone numbers. Section 2 Composition and Information on Ingredients Hazardous Ingredients: None Section 3 Hazards Identification Emergency Overview Colorless; Clear; Liquid; Slight vinegar odor Nonflammable aqueous solution. Does not meet EU, OSHA or WHMIS criteria for hazardous materials. Physical Hazards No physical hazards were determined from a review of available literature. Potential Health Effects Summary This product does not meet EU, OSHA or WHMIS criteria for hazardous materials. Product Hazard Classifications EU: Not applicable WHMIS: Exempt US OSHA: Not applicable Beckman Coulter Safety Rating Flammability (Section V): 0 Health (Section XI): 0 Reactivity with Water (Section X): 0 Contact (Section VIII): 1 Code 0=none 1=slight 2=caution 3=severe Section 4 First Aid Measures Inhalation If product is inhaled, move exposed individual to fresh air. If individual is not breathing, begin artificial respiration immediately and obtain medical attention. Eye Contact If product enters eyes, wash eyes gently under running water for 15 minutes or longer, making sure that the eyelids are held open. If pain or irritation occur, obtain medical attention. VITAMIN B12 Doc. ID: 33000-75 AB Page 26 of 29 VITAMIN B12 (Compartment R1e) Section 4 First Aid Measures (Continued) Skin Contact In case of skin contact, flush with copious amounts of water for at least 15 minutes. If pain or irritation occur, obtain medical attention. Ingestion If ingested, wash mouth out with water. If irritation or discomfort occurs, seek medical attention. Section 5 Fire Fighting Measures Flash Point Not applicable Flammable Limits Not applicable Autoignition Temp. Not applicable Extinguishing Media Use extinguishing media suitable for surrounding fire. Special Fire and Explosion Hazards No special hazards determined. Hazardous Combustion Products Due to the nature and volume of this product, the type and amount of combustion products are negligible. Protective Equipment for Firefighters Self-contained breathing apparatus is recommended for firefighters. Section 6 Accidental Release Measures Personal Precautions No special precautions are necessary. Use good laboratory procedures. Spill and Leak Procedures Wipe up spill and place in container suitable for disposal. Environmental Precautions Contain spill to prevent migration. Section 7 Handling and Storage Handling Precautions No special precautions are necessary; use good laboratory procedures. Recommended Storage Conditions To maintain efficacy, store according to the instructions in the product labeling. Section 8 Exposure Controls and Personal Protection Exposure Limits US OSHA: None established ACGIH: None established DFG MAK: None established Engineering Controls No special engineering controls are required. Use with good general ventilation. Respiratory Protection Under normal conditions, the use of this product should not require respiratory protection. Eye Protection Safety glasses or chemical goggles should be worn to prevent eye contact. VITAMIN B12 Doc. ID: 33000-75 AB Page 27 of 29 VITAMIN B12 (Compartment R1e) Section 8 Exposure Controls and Personal Protection (Continued) Skin Protection Impervious gloves, such as latex or equivalent, should be worn to prevent skin contact. Section 9 Physical and Chemical Properties Physical State Liquid Color Colorless Transparency Clear Odor Slight vinegar odor Odor Threshold Not applicable pH 3.5 Boiling Point Not available Melting Point Not available Specific Gravity 1.00 @20°C Vapor Pressure Not available Vapor Density Not available Evaporation Rate Not available Solubility Water Miscible Organic Not available Section 10 Stability and Reactivity Stability Stable under normal temperatures and pressures. Hazardous Incompatibilities Strong bases Hazardous Decomposition Products When stored as labeled, no known hazardous decomposition products are formed during the shelf-life of this product. Conditions to Avoid Keep away from incompatible material. Section 11 Toxicological Information Toxicity Data for Hazardous Ingredients Not applicable Primary Routes of Exposure The most likely routes of exposure are skin, eye contact and inhalation. Potential Effects of Acute Exposure None identified. Potential Effects of Chronic Exposure None identified. Symptoms of Overexposure No specific symptoms identified. VITAMIN B12 Doc. ID: 33000-75 AB Page 28 of 29 VITAMIN B12 (Compartment R1e) Section 11 Toxicological Information (Continued) Carcinogenicity No ingredients in this product are listed as carcinogens by ACGIH, IARC, NTP, OSHA or 67/548/EEC Annex I. Other Effects None identified. Conditions Aggravated by Exposure None identified. Section 12 Ecological Information Ecotoxicity No information available. Biodegradability No information available. Mobility No information available. Section 13 Disposal Considerations Waste Disposal Dispose of waste product, unused product and contaminated packaging in compliance with federal, state and local regulations. If unsure of the applicable requirements, contact the authorities for information. Section 14 Transport Information Transportation of this product is not regulated under ICAO, IMDG, US DOT, European ADR or Canadian TDG. Section 15 Regulatory Information US Federal and State Regulations SARA 313 No ingredients listed CERCLA RG s, 40 CFR 302.4 Edetic Acid is listed. Acetic Acid is listed. California Proposition 65 No ingredients listed Massachusetts MSL Edetic Acid is listed. Acetic Acid is listed. New Jersey Dept. of Health RTK List Edetic Acid is listed. Acetic Acid is listed. Pennsylvania RTK Edetic Acid is listed. Acetic Acid is listed. EU Labeling Classification Preparation not classified. VITAMIN B12 Doc. ID: 33000-75 AB Page 29 of 29 VITAMIN B12 (Compartment R1e) Section 15 Regulatory Information (Continued) Canada This product is exempt from WHMIS label and MSDS requirements. PIN: Not applicable Ingredients on Ingredient Disclosure List: Acetic Acid Ingredients with unknown toxicological properties: Product is exempt Section 16 Other Information For further information, please contact your local Beckman Coulter representative. WHILE BECKMAN COULTER, INC. BELIEVES THE INFORMATION CONTAINED HEREIN IS VALID AND ACCURATE, BECKMAN COULTER MAKES NO WARRANTY OR REPRESENTATION AS TO ITS VALIDITY, ACCURACY, OR CURRENCY. BECKMAN COULTER SHALL NOT BE LIABLE OR OTHERWISE RESPONSIBLE IN ANYWAY FOR USE OF EITHER THIS INFORMATION OR MATERIALS TO WHICH IT APPLIES. DISPOSAL OF HAZARDOUS MATERIALS MAY BE SUBJECT TO LOCAL LAWS OR REGULATIONS. Printed in U.S.A.