Cell-Freezing Medium B 
(DMSO Freezing Medium, 2X)




List Number:	P133-050

Volume:	50 mL; L-15 Medium containing 20 mM HEPES, 15% DMSO and 2% PVP.    After suspending cells or tissue in Cell-Freezing Medium A (P136-050) add equal parts Cell-Freezing Medium B.  Mix well and store by appropriate methods.

Formulation:	Inorganic Salts	Mg/Liter	Other Compounds	Mg/Liter
NaCl	6,454.00	Alpha-Ketoglutaric Acid	       0
KCl	292	Dihydrostreptomicin Sulfate	0
MgCl2•6H2O	126	Dimethyl Sulfoxide (DMSO)	150 ml
MgSO4•7H2O	126	Fetal Bovine Serum	        0
CaCl2	66.58	Fumaric Acid	        0
Na2HPO4•7H2O	614.17	Galactose	      567
KH2PO4	37.8	HEPES	   5,718.00
NaHCO3	 0	Malic Acid	        0
		Penicillin G-Potassium              0
		Phenol Red	      6.6
		Sodium Pyruvate	     346.5
		PVP                                      20,000   

		
	Amino Acids	Mg/Liter	Vitamins	Mg/Liter
Beta-Alanine	0	D-Calcium Pantothenate	0.63
L-Alpha-Alanine	283.5	Choline Chloride	0.63
L-Arginine•HCl	0	Folic Acid	0.63
L-Arginine (Free Base)	315	Inositol	1.26
L-Asparagine•H2O	157.5	Nicotinamide	0.63
L-Aspartic Acid	0	Pyridoxine•HCl	0.63
L-Cysteine•HCl•H2O	0	Riboflavin	0.06
L-Cysteine (Free Base)	75.6	Thiamine•HCl	0.63
L-Cystine	0
L-Glutamic Acid	0
L-Glutamine	0
Glycine	126
L-Histidine•HCl•H2O	0
L-Histidine (Free Base)	157.5
L-Isoleucine	157.5
L-Leucine	78.8
L-Lysine•HCl	58.6
L-Methionine	94.5
L-Phenylalanine	157.5
L-Proline	0
L-Serine	126
L-Threonine	378
L-Tryptophan	12.6
L-Tyrosine	0
L-Tyrosine•2Na	253.3
L-Valine	126

Storage:	Store at –20°C.

													013100
biosource.com
Biofluids?1114 Taft Street, Rockville, MD 20850?(800) 972-5200? FAX (301) 424-3619
BioSource International, Inc., USA ? 542 Flynn Road, Camarillo, CA 93012 ? (800) 242-0607 ? FAX (805) 987-3385
This product is for research use only.  Not for use in diagnostic procedures.